AG05356
Fibroblast from Skin, Thigh
Description:
LAGOTHRIX
ORDER: PRIMATES
NIA AGING CELL REPOSITORY DNA PANEL - PRIMATE PANEL: PHYLOGENETIC
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Repository
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NIA Aging Cell Culture Repository
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Permit Requirements
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CITES PERMIT REQUIRED IF SHIPPING OUTSIDE OF USA
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| Subcollection |
Animals |
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Biopsy Source
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Thigh
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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62,XX
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Species
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Lagothrix lagotricha
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Common Name
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common woolly monkey
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Remarks
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| PDL at Freeze |
16 |
| Passage Frozen |
7 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Genbank |
COX1 |
| Remark |
The culture was initiated using explants of skin tissue. Cell morphology is fibroblast-like. The karyotype is 62,XX; normal diploid female. |
| Kelley JL, Swanson WJ, Dietary change and adaptive evolution of enamelin in humans and among primates Genetics178:1595-603 2008 |
| PubMed ID: 18245370 |
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| Diehl WE, Stansell E, Kaiser SM, Emerman M, Hunter E, Identification of Post-entry Restrictions to Mason-Pfizer Monkey Virus Infection in New World Monkey Cells Journal of virology82(22):11140-50 2008 |
| PubMed ID: 18799582 |
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| Kaiser SM, Malik HS, Emerman M, Restriction of an extinct retrovirus by the human TRIM5alpha antiviral protein Science (New York, NY)316:1756-8 2007 |
| PubMed ID: 17588933 |
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| Hahn Y, Jeong S, Lee B, Inactivation of MOXD2 and S100A15A by Exon Deletion during Human Evolution Molecular biology and evolution24:2203-12 2007 |
| PubMed ID: 17642472 |
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| Steiper ME, Parikh SJ, Zichello JM, Phylogenetic analysis of the promoter region of the CD40L gene in primates and other mammals Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases8:406-13 2006 |
| PubMed ID: 17275421 |
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| Ireland J, Carlton VE, Falkowski M, Moorhead M, Tran K, Useche F, Hardenbol P, Erbilgin A, Fitzgerald R, Willis TD, Faham M, Large-scale characterization of public database SNPs causing non-synonymous changes in three ethnic groups. Hum Genet119(1-2):75-83 2006 |
| PubMed ID: 16391945 |
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| Salem AH, Ray DA, Hedges DJ, Jurka J, Batzer MA, Analysis of the human Alu Ye lineage. BMC Evol Biol5(1):18 2005 |
| PubMed ID: 15725352 |
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| Sawyer SL, Wu LI, Emerman M, Malik HS, Positive selection of primate TRIM5alpha identifies a critical species-specific retroviral restriction domain. Proc Natl Acad Sci U S A102(8):2832-7 2005 |
| PubMed ID: 15689398 |
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| Sawyer SL, Wu LI, Emerman M, Malik HS, Positive selection of primate TRIM5alpha identifies a critical species-specific retroviral restriction domain. Proc Natl Acad Sci U S A102(8):2832-7 2005 |
| PubMed ID: 15689398 |
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| Ray DA, Xing J, Hedges DJ, Hall MA, Laborde ME, Anders BA, White BR, Stoilova N, Fowlkes JD, Landry KE, Chemnick LG, Ryder OA, Batzer MA, Alu insertion loci and platyrrhine primate phylogeny. Mol Phylogenet Evol35(1):117-26 2005 |
| PubMed ID: 15737586 |
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| Ray DA, Xing J, Hedges DJ, Hall MA, Laborde ME, Anders BA, White BR, Stoilova N, Fowlkes JD, Landry KE, Chemnick LG, Ryder OA, Batzer MA, Alu insertion loci and platyrrhine primate phylogeny. Mol Phylogenet Evol35(1):117-26 2005 |
| PubMed ID: 15737586 |
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| Sawyer SL, Emerman M, Malik HS, Ancient adaptive evolution of the primate antiviral DNA-editing enzyme APOBEC3G. PLoS Biol2(9):E275 2004 |
| PubMed ID: 15269786 |
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| Rockman MV, Hahn MW, Soranzo N, Loisel DA, Goldstein DB, Wray GA, Positive selection on MMP3 regulation has shaped heart disease risk Current biology : CB14:1531-9 2004 |
| PubMed ID: 15341739 |
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| Stedman HH, Kozyak BW, Nelson A, Thesier DM, Su LT, Low DW, Bridges CR, Shrager JB, Minugh-Purvis N, Mitchell MA, Myosin gene mutation correlates with anatomical changes in the human lineage. Nature428(6981):415-8 2004 |
| PubMed ID: 15042088 |
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| Ruiz-Herrera A, García F, Mora L, Egozcue J, Ponsà M, Garcia M, Evolutionary conserved chromosomal segments in the human karyotype are bounded by unstable chromosome bands Cytogenetic and genome research108:161-74 2003 |
| PubMed ID: 15545726 |
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| Frazer KA, Chen X, Hinds DA, Pant PV, Patil N, Cox DR, Genomic DNA insertions and deletions occur frequently between humans and
nonhuman primates. Genome Res13(3):341-6 2003 |
| PubMed ID: 12618364 |
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| Salem AH, Ray DA, Xing J, Callinan PA, Myers JS, Hedges DJ, Garber RK, Witherspoon DJ, Jorde LB, Batzer MA, Alu elements and hominid phylogenetics. Proc Natl Acad Sci U S A100(22):12787-91 2003 |
| PubMed ID: 14561894 |
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| Vincent BJ, Myers JS, Ho HJ, Kilroy GE, Walker JA, Watkins WS, Jorde LB, Batzer MA, Following the LINEs: an analysis of primate genomic variation at human-specific LINE-1 insertion sites. Mol Biol Evol20(8):1338-48 2003 |
| PubMed ID: 12777507 |
| Cumulative PDL at Freeze |
16 |
| Passage Frozen |
7 |
| Split Ratio |
1:6 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
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