AG06205
Fibroblast from Skin, Skin
Description:
ALZHEIMER DISEASE; AD
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Alzheimer's Disease
Alzheimer Disease With No Family History |
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Biopsy Source
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Skin
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Skin
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Race
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White
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY,t(1;13)(1pter>1q21::13q12>13qter;13pter>13q12::1q21>1qter),inv(2)(pter>p13::q11.2>p13::q11.2>qter),t(2;7)(2qter>2p11::7q36>7qter;7pter>7q36::2p11>2pter),t(3;7)(3qter>3p11::7p21>7pter;7qter>7p21::3p11>3pter)[12]/46,XY,t(4;12)(4qter>4p16::12q21>12qter;12pter>12q21::4p16>4pter)[7]/46,XY[31]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
14 |
| Passage Frozen |
12 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The donor showed severe dementia, progressive memory loss, word finding difficulty, dyscalculia and mild to moderate cerebral atrophy on CAT scan. There is no family history of Alzheimer's disease. The culture was initiated on 11/23/82 using explants of minced skin tissue taken antemortem. The morphology is fibroblast-like. The culture is a mosaic with karyotype: 46,XY/46,XY,4p+,12q-/46,XY,t(2;8)(p23;q13); balanced; 70%/14%/ 10% and 6% of cells show random chromosomal abnormalities. Culture was frozen at PDL 11. A lymphoblast culture from same donor is AG06204. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Joshi AU, Van Wassenhove LD, Logas KR, Minhas PS, Andreasson KI, Weinberg KI, Chen CH, Mochly-Rosen D, Aldehyde dehydrogenase 2 activity and aldehydic load contribute to neuroinflammation and Alzheimer's disease related pathology Acta neuropathologica communications7:190 2019 |
| PubMed ID: 31829281 |
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| Khan TK, Alkon DL, An internally controlled peripheral biomarker for Alzheimer's disease: Erk1 and Erk2 responses to the inflammatory signal bradykinin Proceedings of the National Academy of Sciences of the United States of America103:13203-7 2006 |
| PubMed ID: 16920798 |
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| Parshad RP, Sanford KK, Price FM, Melnick LK, Nee LE, Schapiro MB, Tarone RE, Robbins JH, Fluorescent light-induced chromatid breaks distinguish Alzheimer disease cells from normal cells in tissue culture. Proc Natl Acad Sci U S A93:5146-50 1996 |
| PubMed ID: 8643543 |
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| Van Huynh T, Cole G, Katzman R, Huang KP, Saitoh T, Reduced protein kinase C immunoreactivity and altered protein phosphorylation in Alzheimer's disease fibroblasts. Arch Neurol46:1195-9 1989 |
| PubMed ID: 2684108 |
| Cumulative PDL at Freeze |
14 |
| Passage Frozen |
12 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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