AG07102
Fibroblast from Skin, Arm
Description:
MACACA
ORDER: PRIMATES
NIA AGING CELL REPOSITORY DNA PANEL - PRIMATE PANEL: YOUNG AND AGED FOR EACH SPECIES REPRESENTED
Repository
|
NIA Aging Cell Culture Repository
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Permit Requirements
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CITES PERMIT REQUIRED IF SHIPPING OUTSIDE OF USA
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Subcollection |
Animals |
Biopsy Source
|
Arm
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Skin
|
Transformant
|
Untransformed
|
Relation to Proband
|
proband
|
Confirmation
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Clinical summary/Case history
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ISCN
|
42,XY
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Species
|
Macaca nigra
|
Common Name
|
celebes crested macaque
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Remarks
|
|
PDL at Freeze |
10 |
Passage Frozen |
5 |
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IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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Remark |
The skin biopsy was obtained 10/17/83, ante-mortem, from the inner aspect of the upper left arm. The culture was initiated using explants of minced skin tissue. Cell morphology is fibroblast-like. The karyotype is 42,XY; normal diploid male. |
Cumulative PDL at Freeze |
10 |
Passage Frozen |
5 |
Split Ratio |
1:6 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
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