AG08379
Fibroblast from Skin, Arm
Description:
ALZHEIMER DISEASE; AD
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Alzheimer's Disease |
|
Biopsy Source
|
Arm
|
|
Cell Type
|
Fibroblast
|
|
Tissue Type
|
Skin
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Fibroblast from Skin, Arm
|
|
Race
|
White
|
|
Family Member
|
2
|
|
Relation to Proband
|
mother
|
|
Confirmation
|
Clinical summary/Case history
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| PDL at Freeze |
5.83 |
| Passage Frozen |
6 |
| |
| Remarks |
The donor is an unaffected spouse of an Alzheimer's disease affected family member. Donor has two affected children. The skin biopsy was taken ante-mortem from the forearm. Culture was initiated on 8/14/85 using explants of minced skin. The cell morphology is fibroblast-like. Culture was frozen at PDL 5. A lymphoblast culture from same donor is AG08378. Donor's affected daughter is AG08744 Lymphoblast. |
| Oh YM, Lee SW, Kim WK, Chen S, Church VA, Cates K, Li T, Zhang B, Dolle RE, Dahiya S, Pak SC, Silverman GA, Perlmutter DH, Yoo AS, Age-related Huntington's disease progression modeled in directly reprogrammed patient-derived striatal neurons highlights impaired autophagy Nature neuroscience25:1420-1433 2022 |
| PubMed ID: 36303071 |
| |
| Iannuzzi F, Frisardi V, Annunziato L, Matrone C, Might Fibroblasts from Patients with Alzheimer's Disease Reflect the Brain Pathology? A Focus on the Increased Phosphorylation of Amyloid Precursor Protein Tyr Brain sciences11:1420-1433 2020 |
| PubMed ID: 33466666 |
| |
| Sotthibundhu A, Nopparat C, Natphopsuk S, Phuthong S, Noisa P, Govitrapong P, Combination of Melatonin and Small Molecules Improved Reprogramming Neural Cell Fates via Autophagy Activation Neurochemical research11:1420-1433 2020 |
| PubMed ID: 34165669 |
| |
| Meyer K, Feldman HM, Lu T, Drake D, Lim ET, Ling KH, Bishop NA, Pan Y, Seo J, Lin YT, Su SC, Church GM, Tsai LH, Yankner BA, REST and Neural Gene Network Dysregulation in iPSC Models of Alzheimer's Disease Cell reports26:1112-1127.e9 2018 |
| PubMed ID: 30699343 |
| |
| Sarasija S, Laboy JT, Ashkavand Z, Bonner J, Tang Y, Norman KR, Presenilin mutations deregulate mitochondrial Ca eLife7:1112-1127.e9 2017 |
| PubMed ID: 29989545 |
| Passage Frozen |
6 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Supplement |
- |
|
|