AG12417
Endothelial from Aorta, thoracic
Description:
SUS
ORDER: CETARTIODACTYLA
Repository
|
NIA Aging Cell Culture Repository
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Subcollection |
Animals |
Biopsy Source
|
Aorta, thoracic
|
Cell Type
|
Endothelial
|
Tissue Type
|
Artery
|
Transformant
|
Untransformed
|
Sample Source
|
Endothelial from Aorta, thoracic
|
Ethnicity
|
YORKSHIRE
|
Family Member
|
1
|
Relation to Proband
|
proband
|
Confirmation
|
Clinical summary/Case history
|
ISCN
|
38,XX/39,XX,+17
|
Species
|
Sus scrofa
|
Common Name
|
pig
|
Remarks
|
|
PDL at Senescence |
29 |
PDL at Freeze |
14 |
Passage Frozen |
9 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
|
Remarks |
Yorkshire breed. The thoracic aorta was removed immediately post-mortem. The culture was initiated using cells released from the lumen of the vessel by collagenase digestion. The cell morphology is polygonal. The culture tested positive for the endothelial cell-specific von Willebrand factor and negative for alpha-smooth muscle actin (immunofluorescence assays). The culture was grown on gelatin-coated flasks and the medium was supplemented with ECGS (0.02 mg/ml) and heparin (0.05 mg/ml). Culture medium: HF12 with 10% plasma-derived donor horse serum with supplements. The HF12 medium should be endotoxin-free. The culture is a mosaic with karyotype: 38,XX/39/XX,+17; 86%/14%. Other cultures from this pig are AG12362, AG12363, and AG12372. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
PDL at Senescence |
29 |
Cumulative PDL at Freeze |
14 |
Passage Frozen |
9 |
Split Ratio |
1:2 |
Temperature |
37 C |
Percent CO2 |
5% |
Percent O2 |
AMBIENT |
Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
Serum |
10% fetal bovine serum |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
Endothelial Cell Growth Supplement 0.02 mg/ml |
Supplement |
Heparin 0.05 mg/ml |
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