AG14486
Fibroblast from Umbilical Cord, Umbilical cord
Description:
APPARENTLY HEALTHY INDIVIDUAL
Repository
|
NIA Aging Cell Culture Repository
|
Subcollection |
Apparently Healthy Collection |
Biopsy Source
|
Umbilical cord
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Umbilical Cord
|
Transformant
|
Untransformed
|
Sample Source
|
Fibroblast from Umbilical Cord, Umbilical cord
|
Relation to Proband
|
proband
|
Confirmation
|
Clinical summary/Case history
|
ISCN
|
46,XX
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
PDL at Freeze |
4 |
Passage Frozen |
3 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
|
Remarks |
The culture was initiated on 2/3/97 from explants of minced umbilical cord. The cell morphology is fibroblast-like. The karyotype is 46,XX with 6% of the cells examined showing random chromosome loss. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
Cumulative PDL at Freeze |
4 |
Passage Frozen |
3 |
Split Ratio |
1:2 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
- |
|
|