Description:
APPARENTLY HEALTHY INDIVIDUAL
Repository
|
NIA Aging Cell Culture Repository
|
Subcollection |
Apparently Healthy Collection |
Cell Type
|
Fibroblast
|
Transformant
|
Untransformed
|
Family Member
|
1
|
Relation to Proband
|
proband
|
Confirmation
|
Clinical summary/Case history
|
ISCN
|
46,XX
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
Passage Frozen |
2 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
|
Remarks |
The culture was initiated on 4/11/97 from explants of minced umbilical cord removed ante-mortem. The cell morphology is fibroblast-like. The karyotype is 46,XX with 6% of the cells examined showing random chromosome loss/gain and 2% showing random chromosomal aberrations. An endothelial cell culture from this same donor is AG14572. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
Passage Frozen |
2 |
Split Ratio |
1:4 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
- |
|
|