AG14814
LCL from B-Lymphocyte
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
"Intra-abdominal fat and risk of disease in children and adolescents" study
Apparently Healthy Collection |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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Black/African American
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Ethnicity
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AFRICAN-AMERICAN
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY,21s+
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Participant in the "intra-abdominal fat and risk of disease in children and adolescents" study. The culture was initiated on 11/13/97 by transformation of lymphocytes with Epstein Barr virus. The culture grows in suspension and the cell morphology is spherical. The karyotype is 46,XY,21ps+ with 8% of the cells examined showing random chromosome loss/gain. Lymphoblast cultures from this donor's fraternal twin sister and a brother are AG14815 and AG14816, respectively. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Goran MI, Metabolic precursors and effects of obesity in children: a decade of progress, 1990-1999. Am J Clin Nutr73(2):158-71 2001 |
| PubMed ID: 11157310 |
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| Goran MI, Bergman RN, Gower BA, Influence of total vs. visceral fat on insulin action and secretion in African American and white children. Obes Res9(8):423-31 2001 |
| PubMed ID: 11500522 |
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| Goran MI, Gower BA, Longitudinal study on pubertal insulin resistance. Diabetes50(11):2444-50 2001 |
| PubMed ID: 11679420 |
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| Herd SL, Gower BA, Dashti N, Goran MI, Body fat, fat distribution and serum lipids, lipoproteins and apolipoproteins in African-American and Caucasian-American prepubertal children. Int J Obes Relat Metab Disord25(2):198-204 2001 |
| PubMed ID: 11410820 |
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| Huang TT, Johnson MS, Figueroa-Colon R, Dwyer JH, Goran MI, Growth of visceral fat, subcutaneous abdominal fat, and total body fat in children. Obes Res9(5):283-9 2001 |
| PubMed ID: 11346669 |
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| Johnson MS, Figueroa-Colon R, Huang TT, Dwyer JH, Goran MI, Longitudinal changes in body fat in African American and Caucasian children: influence of fasting insulin and insulin sensitivity. J Clin Endocrinol Metab86(7):3182-7 2001 |
| PubMed ID: 11443186 |
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| Li C, Johnson MS, Goran MI, Effects of low birth weight on insulin resistance syndrome in caucasian and African-American children. Diabetes Care24(12):2035-42 2001 |
| PubMed ID: 11723079 |
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| Sun M, Gower BA, Bartolucci AA, Hunter GR, Figueroa-Colon R, Goran MI, A longitudinal study of resting energy expenditure relative to body composition during puberty in African American and white children. Am J Clin Nutr73(2):308-15 2001 |
| PubMed ID: 11157329 |
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| Goran MI, Visceral fat in prepubertal children: Influence of obesity, anthropometry, ethnicity, gender, diet, and growth. Am J Human Biol11(2):201-207 1999 |
| PubMed ID: 11533944 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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