AG18256
LCL from B-Lymphocyte
Description:
CENTENARIAN STUDY NORMAL CONTROL
GEORGIA CENTENARIAN COLLECTION- PANEL OF 78 AFRICAN-AMERICAN CONTROLS
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
University of Georgia Centenarian Study |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Race
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Black/African American
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Clinically normal; age verified; donor reports no relatives over 90 years of age |
| Spellman C, Ahmed MM, Dubach D, Gardiner KJ, Expression of trisomic proteins in Down syndrome model systems Gene512:219-25 2012 |
| PubMed ID: 23103828 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
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