GM03468
Fibroblast from Skin, Foreskin
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Foreskin
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Foreskin
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
12 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase and Glucose-6-Phosphate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Foreskin; sensitive to azaguanine and thioguanine; utilizes exogenous hypoxanthine; normal HPRT and APRT; 46,XY |
| Pachernegg S, Robevska G, G A Ferreira L, van den Bergen JA, Vlahos K, Howden SE, Sinclair AH, Ayers KL, Generation of heterozygous (MCRIi031-A-1) and homozygous (MCRIi031-A-2) SOX9 knockout human iPSC lines Stem cell research79:103484 2024 |
| PubMed ID: 38924973 |
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| Rice MC, Smith ST, Bullrich F, Havre P, Kmiec EB, Isolation of human and mouse genes based on homology to REC2, a recombinational repair gene from the fungus Ustilago maydis. Proc Natl Acad Sci U S A94:7417-7422 1997 |
| PubMed ID: 9207106 |
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| Yancey PG, Rodrigueza WV, Kilsdonk EPC, Stoudt GW, Johnson WJ, Phillips MC, Rothblat GH, Cellular cholesterol efflux mediated by cyclodextrins. Demonstration Of kinetic pools and mechanism of efflux. J Biol Chem271:16026-34 1996 |
| PubMed ID: 8663188 |
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| Johnson WJ, Fischer RT, Phillips MC, Rothblat GH, Efflux of newly synthesized cholesterol and biosynthetic sterol intermediates from cells. Dependence on acceptor type and on enrichment of cells with cholesterol. J Biol Chem270:25037-46 1995 |
| PubMed ID: 7559634 |
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| Wang D, Smith CL, Large-scale structure conservation along the entire long arm of human chromosome 21. Genomics20:441-51 1994 |
| PubMed ID: 8034317 |
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| Ogura K, Ogura M, Anderson RL, Sweeley CC, Peroxidase-amplified assay of sialidase activity toward gangliosides. Anal Biochem200:52-7 1992 |
| PubMed ID: 1595901 |
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| Swamy MJ, Sweeley CC, On the metabolism of GM3 ganglioside in cultured human foreskin fibroblasts. Biochem Biophys Res Commun162:1188-93 1989 |
| PubMed ID: 2764928 |
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| Weiss MJ, Ray K, Henthorn PS, Lamb B, Kadesch T, Harris H, Structure of the human liver/bone/kidney alkaline phosphatase gene. J Biol Chem263:12002-10 1988 |
| PubMed ID: 3165380 |
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| Pentchev PG, Comly ME, Kruth HS, Patel S, Proestel M, Weintroub H, The cholesterol storage disorder of the mutant BALB/c mouse. A primary genetic lesion closely linked to defective esterification of exogenously derived cholesterol and its relationship to human type C Niemann-Pick disease. J Biol Chem261:2772-7 1986 |
| PubMed ID: 3949747 |
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| Wolfbauer G, Glick JM, Minor LK, Rothblat GH, Development of the smooth muscle foam cell: uptake of macrophage lipid inclusions. Proc Natl Acad Sci U S A83:7760-4 1986 |
| PubMed ID: 3020555 |
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| Woodhead, Avril D., Blackett, Anthony D., and Hollaender, Alexander (editors), Molecular Biology of Aging Proc Natl Acad Sci U S A83:pp 315-344 1985 |
| PubMed ID: 3020555 |
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| Jacobs L, Bean CL, Marx JA, Optimal phenotypic expression times for HPRT mutants induced in foreskin-, skin-, and lung-derived human diploid fibroblasts. Environ Mutagen5:717-31 1983 |
| PubMed ID: 6617601 |
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| Tarone RE, Scudiero DA, Robbins JH, Statistical methods for in vitro cell survival assays. Mutat Res111:79-96 1983 |
| PubMed ID: 6621576 |
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| Steglich C, DeMars R, Mutations causing deficiency of APRT in fibroblasts cultured from human heterozygous for mutant APRT alleles. Somatic Cell Genet8:115-41 1982 |
| PubMed ID: 7101101 |
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| Strom S, Michalopoulos G, Mutagenesis and DNA binding of benzo(a)pyrene in cocultures of rat hepatocytes and human fibroblasts. Cancer Res42:4519-24 1982 |
| PubMed ID: 6290039 |
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| Michalopoulos G, Strom SC, Kligerman AD, Irons GP, Novicki DL, Mutagenesis induced by procarcinogens at the hypoxanthine-guanine phosphoribosyl transferase locus of human fibroblasts cocultured with rat hepatocytes. Cancer Res41:1873-8 1981 |
| PubMed ID: 7214354 |
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| Scudiero DA, Meyer SA, Clatterbuck BE, Tarone RE, Robbins JH, Hypersensitivity to N-methyl-N'-nitro-N-nitrosoguanidine in fibroblasts from patients with Huntington disease, familial dysautonomia, and other primary neuronal degenerations. Proc Natl Acad Sci U S A78:6451-5 1981 |
| PubMed ID: 6458814 |
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| Kligerman AD, Strom SC, Michalopoulos G, Sister chromatid exchange studies in human fibroblast-rat hepatocyte co-cultures: a new in vitro system to study SCEs. Environ Mutagen2:157-165 1980 |
| PubMed ID: 7327165 |
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| Jacobs L, Demars R, Quantification of chemical mutagenesis in diploid human fibroblasts: induction of azaguanine-resistant mutants by N-methyl-N'-nitro-N- nitrosoguanidine. Mutat Res53:29-53 1978 |
| PubMed ID: 625304 |
| Passage Frozen |
12 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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