GM03550
Fibroblast from Skin, Unspecified
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Animals |
|
Biopsy Source
|
Unspecified
|
|
Cell Type
|
Fibroblast
|
|
Tissue Type
|
Skin
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Fibroblast from Skin, Unspecified
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
Species
|
Ovis aries
|
|
Common Name
|
sheep
|
|
Remarks
|
|
| Passage Frozen |
2 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis |
| |
| GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME |
PCR analysis of DNA from this cell culture gave a negative result with a primer for Yq11, DYS227. |
| |
| Genbank |
COX1 |
| Remarks |
Southdown sheep; 4 month old female; fibroblast culture; this culture was characterized by immunocytochemistry using the TE-7 anti-fibroblast antibody and pancytokeratin epithelial cell marker and found to have 1% epithelial cells |
| Syedain ZH, Haynie B, Johnson SL, Lahti M, Berry J, Carney JP, Li J, Hill RC, Hansen KC, Thrivikraman G, Bianco R, Tranquillo RT, Pediatric tri-tube valved conduits made from fibroblast-produced extracellular matrix evaluated over 52 weeks in growing lambs Science translational medicine13: 2020 |
| PubMed ID: 33731437 |
| |
| Davis T, Skinner JW, Faragher RG, Jones CJ, Kipling D, Replicative senescence in sheep fibroblasts is a p53 dependent process. Exp Gerontol40(1-2):17-26 2005 |
| PubMed ID: 15664728 |
| Passage Frozen |
2 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|
|