GM10612
Somatic cell hybrid
Description:
ADDITIONAL HUMAN/CHINESE HAMSTER SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Family Member
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19
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Confirmation
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Karyotypic analysis and Southern blot hybridization
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ISCN
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Human/Chinese hamster somatic cell hybrid retaining human chromosome #19 in 96% of cells
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for Xq28, DXS15. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 3p25, RAF1, and 3q26.2qter, TFRC. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 4p16.2p15.1, D4S20, and 4q21q23, ADH3. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 5pterp15.3, D5S10, and 5q33q35, PDGFRB. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 9 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 9q34, D9S7, & 9q34.1, ABL. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 11p13, CAT. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 17p13.1, MYH2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 19 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 19p13.3p13.2, C3; 19q13.3, LHB; and 19p13.1, D19S11. |
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| Remarks |
Line PK-87-19; produced by the fusion of retroviral vector SP-1 infected human lymphoblasts, GM07890, 46,XX,del(13), with CHO cell line UV-135; selected in medium containing histidinol; retains human chromosome #19 in 96% of cells |
| Anderson MJ, Viars CS, Czekay S, Cavenee WK, Arden KC, Cloning and characterization of three human forkhead genes that comprise an FKHR-like gene subfamily. Genomics47:187-99 1998 |
| PubMed ID: 9479491 |
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| Katsanis N, Fisher EM, A novel C-terminal binding protein (CTBP2) is closely related to CTBP1, an adenovirus E1A-binding protein, and maps to human chromosome 21q21.3. Genomics47:294-9 1998 |
| PubMed ID: 9479502 |
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| Trask BJ, Friedman C, Martin-Gallardo A, Rowen L, Akinbami C, Blankenship J, Collins C, Giorgi D, Iadonato S, Johnson F, Kuo WL, Massa H, Morrish T, Naylor S, Nguyen OT, Rouquier S, Smith T, Wong DJ, Youngblom J, van den Engh G, Members of the olfactory receptor gene family are contained in large blocks of DNA duplicated polymorphically near the ends of human chromosomes. Hum Mol Genet7:13-26 1998 |
| PubMed ID: 9384599 |
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| Davies JP, Cotter PD, Ioannou YA, Cloning and mapping of human Rab7 and Rab9 cDNA sequences and identification of a Rab9 pseudogene. Genomics41:131-4 1997 |
| PubMed ID: 9126495 |
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| Hansen L, Arden KC, Rasmussen SB, Viars CS, Vestergaard H, Hansen T, Moller AM, Woodgett JR, Pedersen O, Chromosomal mapping and mutational analysis of the coding region of the glycogen synthase kinase-3alpha and beta isoforms in patients with NIDDM. Diabetologia40:940-6 1997 |
| PubMed ID: 9267989 |
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| Jones MH, Zhang Y, Tirosvoutis KN, Davey PM, Webster AR, Walsh D, Spurr NK, Affara NA, Chromosomal assignment of 311 sequences transcribed in human adult testis. Genomics40:155-67 1997 |
| PubMed ID: 9070934 |
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| McCusker D, Jones T, Sheer D, Trowsdale J, Genetic relationships of the genes encoding the human proteasome beta subunits and the proteasome PA28 complex. Genomics45:362-7 1997 |
| PubMed ID: 9344661 |
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| Jones T, Sheer D, Kapetanopoulos A, Hauber J, Bevec D, Steinkasserer A, The gene coding for human deoxyhypusine synthase (DHPS) maps to chromosome 19p13.11-p13.12. Genomics35:635-7 1996 |
| PubMed ID: 8812510 |
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| Li QY, Lennon GG, Brook JD, The identification of exons from the MED/PSACH region of human chromosome 19. Genomics32:218-24 1996 |
| PubMed ID: 8833148 |
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| Arden KC, Viars CS, Weiss S, Argentin S, Nemer M, Localization of the human B-type natriuretic peptide precursor (NPPB) gene to chromosome 1p36. Genomics26:385-9 1995 |
| PubMed ID: 7601467 |
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| Bermingham JR Jr, Arden KC, Naumova AK, Sapienza C, Viars CS, Fu XD, Khotz J, Manley JL, Rosenfeld MG, Chromosomal localization of mouse and human genes encoding the splicing factors ASF/SF2 (SFRS1) and SC-35 (SFRS2). Genomics29:70-9 1995 |
| PubMed ID: 8530103 |
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| Kelsell DP, Rooke L, Warne D, Bouzyk M, Cullin L, Cox S, West L, Povey S, Spurr NK, Development of a panel of monochromosomal somatic cell hybrids for rapid gene mapping Annals of human genetics59:233-41 1995 |
| PubMed ID: 7625768 |
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| De Plaen E, Arden K, Traversari C, Gaforio JJ, Szikora JP, De Smet C, Brasseur F, van der Bruggen P, Lethé B, Lurquin C, Structure, chromosomal localization, and expression of 12 genes of the MAGE family Immunogenetics40:360-9 1994 |
| PubMed ID: 7927540 |
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| MacCollin M, Peterfreund R, MacDonald M, Fink JS, Gusella J, Mapping of a human A2a adenosine receptor (ADORA2) to chromosome 22. Genomics20:332-3 1994 |
| PubMed ID: 8020991 |
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| Weiss J, Rubinfeld B, Polakis PG, McCormick F, Cavenee WK, Arden KC, The RAP1GA1 locus for human Rap1-GTPase activating protein 1 maps to chromosome 1p36.1-->p35. Cytogenet Cell Genet66:18-21 1994 |
| PubMed ID: 8275700 |
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| Howe JR, Lairmore TC, Veile R, Dou S, Wells SA Jr, Donis-Keller H, Development of a sequence-tagged site for the centromere of chromosome 10: its use in cytogenetic and physical mapping. Hum Genet91:199-204 1993 |
| PubMed ID: 8478002 |
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| McClatchey AI, Cannon SC, Slaugenhaupt SA, Gusella JF, The cloning and expression of a sodium channel beta 1-subunit cDNA from human brain. Hum Mol Genet2:745-9 1993 |
| PubMed ID: 8394762 |
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| Washington SS, Bowcock AM, Gerken S, Matsunami N, Lesh D, Osborne-Lawrence SL, Cowell J, Ledbetter DH, White RL, Chakravarti A, A somatic cell hybrid map of human chromosome 13. Genomics18:486-95 1993 |
| PubMed ID: 8307557 |
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| Kremer EJ, Kalatzis V, Baker E, Callen DF, Sutherland GR, Maliszewski CR, The gene for the human IgA Fc receptor maps to 19q13.4. Hum Genet89:107-8 1992 |
| PubMed ID: 1577457 |
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| Ledbetter SA, Garcia-Heras J, Ledbetter DH, "PCR-karyotype" of human chromosomes in somatic cell hybrids. Genomics8:614-22 1990 |
| PubMed ID: 2276735 |
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| Warburton D, Gersen S, Yu MT, Jackson C, Handelin B, Housman D, Monochromosomal rodent-human hybrids from microcell fusion of human lymphoblastoid cells containing an inserted dominant selectable marker. Genomics6:358-66 1990 |
| PubMed ID: 2307476 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Histidinol 4mM |
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