GM10660
Somatic cell hybrid
Description:
CHROMOSOME 1 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 17 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Hybrids |
|
Cell Type
|
Somatic cell hybrid
|
|
Transformant
|
Untransformed
|
|
Confirmation
|
Karyotypic analysis and Southern blot hybridization
|
|
ISCN
|
Mouse/human hybrid retaining human der(1)t(1;17) chromosome (100% of cells) & #14 (40% of cells)
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 1p36, PND, and gave a positive result with a probe for 1q32, REN. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 6 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 6q24q27, ESR. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 17p13.1, MYH2, and gave a positive result with a probe for 17q21.3q23, MPO. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 17q25-qter, D17S784, and 17q, GAS, and gave a negative result with a primer for 17p13.1, TP53. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 22q13, D22S45, and 22q12.3q13.1, PDGFB. |
| |
| Remarks |
Line DCR-1; produced by the PEG fusion of lymphoblasts from a neurofibromatosis patient, t(1;17)(p34.3;q11.2), with TKdef Cl1D mouse cells; selected in HAT medium; retains the der(1) chromosome in 100% of cells & #14 in 40% of cells |
| Coskun AK, van Maanen M, Nguyen V, Sutton RE, Human chromosome 2 carries a gene required for production of infectious human immunodeficiency virus type 1 Journal of virology80:3406-15 2006 |
| PubMed ID: 16537608 |
| |
| Moran JL, Johnston SH, Rauskolb C, Bhalerao J, Bowcock AM, Vogt TF, Genomic structure, mapping, and expression analysis of the mammalian Lunatic,
Manic, and Radical fringe genes. Mamm Genome10(6):535-41 1999 |
| PubMed ID: 10341080 |
| |
| Plummer SJ, Simmons JA, Adams L, Casey G, Mapping of 228 ESTs and 26 genes into an integrated physical and genetic map of human chromosome 17. Genomics45:140-6 1997 |
| PubMed ID: 9339370 |
| |
| Cerosaletti KM, Shapero MH, Fournier RE, Cloning mammary cell cDNAs from 17q12-q23 using interspecific somatic cell hybrids and subtractive hybridization. Genomics25:226-37 1995 |
| PubMed ID: 7774923 |
| |
| Flejter WL, Bennett-Baker P, Barcroft CL, Kiousis S, Chamberlain JS, Region-specific cosmids and STRPs identified by chromosome microdissection and FISH. Genomics25:413-20 1995 |
| PubMed ID: 7789975 |
| |
| Polymeropoulos MH, Torres R, Yanovski JA, Chandrasekharappa SC, Ledbetter DH, The human corticotropin-releasing factor receptor (CRHR) gene maps to chromosome 17q12-q22. Genomics28:123-4 1995 |
| PubMed ID: 7590738 |
| |
| Umbricht CB, Griffin CA, Hawkins AL, Grzeschik KH, O'Connell P, Leach R, Green ED, Kelly TJ, High-resolution genomic mapping of the three human replication protein A genes (RPA1, RPA2, and RPA3). Genomics20:249-57 1994 |
| PubMed ID: 8020972 |
| |
| Anderson LA, Friedman L, Osborne-Lawrence S, Lynch E, Weissenbach J, Bowcock A, King MC, High-density genetic map of the BRCA1 region of chromosome 17q12-q21. Genomics17:618-23 1993 |
| PubMed ID: 8244378 |
| |
| Dorig RE, Marcil A, Chopra A, Richardson CD, The human CD46 molecule is a receptor for measles virus (Edmonston strain). Cell75:295-305 1993 |
| PubMed ID: 8402913 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
|
|