GM10663
Somatic cell hybrid
Description:
CHROMOSOME X REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Human/mouse hybrid retaining human del(X) & chromosomes #6, 11, 17p-, & 21
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for Xp11.21, ALAS2, Xp11.3, DXS1003, Xp22.32, STS, Xq12, DXS453, Xq13.3, PGK1, Xq21.3, DXS3, Xq23, DXS1059, and Xq26, HPRT, and gave a negative result with a primer for Xq28, G6PD. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1 |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 1p13, NGFB. |
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| Remarks |
Line 2384-A2; produced by the PEG fusion of lymphos from a pt with hemophilia B, del(X)(q27), with HPRT-deficient Chinese hamster RJK88 cells; grown in HZ medium; retains human #6 (100%), 11 (96%), 17p(28%), 21 (84%) & del(X) (96% of cells) |
| Oaks MK, Hanson JP Jr, O'Malley DP, Molecular cytogenetic mapping of the human melanoma antigen (MAGE) gene family to chromosome region Xq27-qter: implications for MAGE immunotherapy. Cancer Res54:1627-9 1994 |
| PubMed ID: 8137270 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Azaserine 4.4 x 10-5M |
| Supplement |
Hypoxanthine 1 x 10-4M |
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