GM11943
Somatic cell hybrid
Description:
CHROMOSOME 11 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 2 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Hybrids |
|
Cell Type
|
Somatic cell hybrid
|
|
Transformant
|
Untransformed
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
Mouse/human hybrid retaining a human der(11) chromosome & human #4, 5, 6, 7, 10, 13, 14, & X
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 2p25, D2S1, and gave a negative result with a probe for 2q37, D2S3. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 2p13, TGFA, and 2p23, POMC, and gave negative results with primers for 2q13, IL1A, and 2q37, ALPP. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 4p16.2p15.1, D4S20. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 11p15.5, D11S12, and gave a positive result with a probe for 11q23.3, ETS1. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 11p13, CAT, and 11q21-q22, CLG. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 16q13q22.1, MT2, and 16pterp13, D16S36. |
| |
| Remarks |
Line POR4; produced by the fusion of human fibro, GM04613, 46,XY,t(2;11)(p11; p13) with HPRT-deficient mouse RAG cells; grown in medium without selection; retains human der(11)t(2;11) in 92% of cells & other human chromosomes |
| Leek J, Lench N, Maraj B, Bailey A, Carr IM, Andersen S, Cross J, Whelan P, MacLennan KA, Meredith DM, Prostate-specific membrane antigen: evidence for the existence of a second related human gene British journal of cancer72:583-8 1995 |
| PubMed ID: 7669565 |
| |
| Junien C, van Heyningen V, Evans G, Little P, Mannens M, Report of the second chromosome 11 workshop. Genomics12:620-5 1992 |
| PubMed ID: 1348491 |
| |
| Bickmore WA, Porteous DJ, Christie S, Seawright A, Fletcher JM, Maule JC, Couillin P, Junien C, Hastie ND, van Heyningen V, CpG islands surround a DNA segment located between translocation breakpoints associated with genitourinary dysplasia and aniridia. Genomics5:685-93 1989 |
| PubMed ID: 2556343 |
| |
| Bickmore W, Christie S, van Heyningen V, Hastie ND, Porteous DJ, Hitch-hiking from HRAS1 to the WAGR locus with CMGT markers. Nucleic Acids Res16:51-60 1988 |
| PubMed ID: 2829125 |
| |
| Seawright A, Fletcher JM, Fantes JA, Morrison H, Porteous DJ, Li SS, Hastie ND, Van Heyningen V, Analysis of WAGR deletions and related translocations with gene- specific DNA probes, using FACS-selected cell hybrids. Somat Cell Mol Genet14:21-30 1988 |
| PubMed ID: 2829363 |
| |
| Porteous DJ, Bickmore W, Christie S, Boyd PA, Cranston G, Fletcher JM, Gosden JR, Rout D, Seawright A, Simola KO, et al, HRAS1-selected chromosome transfer generates markers that colocalize aniridia- and genitourinary dysplasia-associated translocation breakpoints and the Wilms tumor gene within band 11p13. Proc Natl Acad Sci U S A84:5355-9 1987 |
| PubMed ID: 3037545 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|
|