GM12082
Somatic cell hybrid
Description:
ADDITIONAL HUMAN/CHINESE HAMSTER SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Family Member
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18
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/Chinese hamster hybrid retaining human chromosome #18
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 8 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 8p21, NEFL. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 18q12.3, D18S6, and 18q23, MBP. PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 18p11.31-p11.22, TYMS. |
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| Remarks |
Line DL18ts; produced by the fusion of human lymphoblast cells, 46,XX,del(5), with the temperature-sensitive Chinese hamster ovary cell line tsH1; grown in medium with proline at 33C; retains human chromosome #18 in 80% of cells |
| Davies JP, Cotter PD, Ioannou YA, Cloning and mapping of human Rab7 and Rab9 cDNA sequences and identification of a Rab9 pseudogene. Genomics41:131-4 1997 |
| PubMed ID: 9126495 |
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| de Coo R, Buddiger P, Smeets H, Geurts van Kessel A, Morgan-Hughes J, Weghuis DO, Overhauser J, van Oost B, Molecular cloning and characterization of the active human mitochondrial NADH:ubiquinone oxidoreductase 24-kDa gene (NDUFV2) and its pseudogene. Genomics26:461-6 1995 |
| PubMed ID: 7607668 |
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| van Kessel AG, Straub RE, Silverman GA, Gerken S, Overhauser J, Report and abstracts of the second international workshop on human chromosome 18 mapping. Doorwerth, the Netherlands, July 19-20, 1993. Cytogenet Cell Genet65:142-65 1994 |
| PubMed ID: 8222751 |
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| Markie D, Jones TA, Sheer D, Bodmer WF, A somatic cell hybrid panel for regional mapping of human chromosome 18. Genomics14:431-6 1992 |
| PubMed ID: 1427858 |
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| Bicknell DC, Markie D, Spurr NK, Bodmer WF, The human chromosome content in human x rodent somatic cell hybrids analyzed by a screening technique using Alu PCR. Genomics10:186-92 1991 |
| PubMed ID: 2045101 |
| Split Ratio |
1:5 |
| Temperature |
33 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 1 x 10-4M |
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