GM14854
Somatic cell hybrid
Description:
CHROMOSOME 1 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 2 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/mouse hybrid retaining a human der(2)t(1;2)(p21;q37) in 92% of cells and other human chromosomes
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Line JWR-22H; produced by fusing human lymphocytes, 2pter>2q37::1p21>1pter, with HGPRT- RAG mouse cells; grown in HAT medium; 92% of cells retain a human der(2)t(1;2)(p21;q37) & other human chromosomes |
| Mahnke-Zizelman DK, Eddy R, Shows TB, Sabina RL, Characterization of the human AMPD3 gene reveals that 5' exon useage is subject to transcriptional control by three tandem promoters and alternative splicing. Biochim Biophys Acta1306:75-92 1996 |
| PubMed ID: 8611627 |
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| Chang ML, Eddy RL, Shows TB, Lau JT, Three genes that encode human beta-galactoside alpha 2,3- sialyltransferases. Structural analysis and chromosomal mapping studies. Glycobiology5:319-25 1995 |
| PubMed ID: 7655169 |
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| Maki RG, Eddy RL Jr, Byers M, Shows TB, Srivastava PK, Mapping of the genes for human endoplasmic reticular heat shock protein gp96/grp94. Somat Cell Mol Genet19:73-81 1993 |
| PubMed ID: 8460400 |
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| Hautala T, Byers MG, Eddy RL, Shows TB, Kivirikko KI, Myllyla R, Cloning of human lysyl hydroxylase: complete cDNA-derived amino acid sequence and assignment of the gene (PLOD) to chromosome 1p36.3---- p36.2. Genomics13:62-9 1992 |
| PubMed ID: 1577494 |
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| Bloch KD, Hong CC, Eddy RL, Shows TB, Quertermous T, cDNA cloning and chromosomal assignment of the endothelin 2 gene: vasoactive intestinal contractor peptide is rat endothelin 2. Genomics10:236-42 1991 |
| PubMed ID: 1840558 |
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| Collins T, Williams A, Johnston GI, Kim J, Eddy R, Shows T, Gimbrone MA Jr, Bevilacqua MP, Structure and chromosomal location of the gene for endothelial- leukocyte adhesion molecule 1. J Biol Chem266:2466-73 1991 |
| PubMed ID: 1703529 |
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| Kallunki P, Eddy RL, Byers MG, Kestila M, Shows TB, Tryggvason K, Cloning of human heparan sulfate proteoglycan core protein, assignment of the gene (HSPG2) to 1p36.1----p35 and identification of a BamHI restriction fragment length polymorphism. Genomics11:389-96 1991 |
| PubMed ID: 1685141 |
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| Lau KS, Eddy RL, Shows TB, Fisher CW, Chuang DT, Cox RP, Localization of the dihydrolipoamide branched-chain transacylase gene (DBT) of the human branched-chain keto acid dehydrogenase complex to chromosome 1. Cytogenet Cell Genet56:33-5 1991 |
| PubMed ID: 2004553 |
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| Perl A, Isaacs CM, Eddy RL, Byers MG, Sait SN, Shows TB, The human T-cell leukemia virus-related endogenous sequence (HRES1) is located on chromosome 1 at q42. Genomics11:1172-3 1991 |
| PubMed ID: 1783388 |
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| Jenkins RN, Osborne-Lawrence SL, Sinclair AK, Eddy RL Jr, Byers MG, Shows TB, Duby AD, Structure and chromosomal location of the human gene encoding cartilage matrix protein. J Biol Chem265:19624-31 1990 |
| PubMed ID: 2246248 |
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| Tenen DG, Satterthwaite AB, Borson R, Simmons D, Eddy RL, Shows TB, Chromosome 1 localization of the gene for CD34, a surface antigen of human stem cells. Cytogenet Cell Genet53:55-7 1990 |
| PubMed ID: 1691071 |
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| Wade R, Eddy R, Shows TB, Kedes L, cDNA sequence, tissue-specific expression, and chromosomal mapping of the human slow-twitch skeletal muscle isoform of troponin I. Genomics7:346-57 1990 |
| PubMed ID: 2365354 |
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| Tepler I, Morton CC, Shimizu A, Holcombe RF, Eddy R, Shows TB, Leder P, The gene for the human mast cell high-affinity IgE receptor alpha chain: chromosomal localization to Iq21-q23 and RFLP analysis. Am J Hum Genet45:761-5 1989 |
| PubMed ID: 2573277 |
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| Shows TB, Eddy RL, Byers MG, Fukushima Y, Dehaven CR, Murray JC, Bell GI, Polymorphic human glucose transporter gene (GLUT) is on chromosome 1p31.3----p35. Diabetes36:546-9 1987 |
| PubMed ID: 3028891 |
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| Fowler ML, Nakai H, Byers MG, Fukushima H, Eddy RL, Henry WM, Haley LL, O'Brien JS, Shows TB, Chromosome 1 localization of the human alpha-L-fucosidase structural gene with a homologous site on chromosome 2. Cytogenet Cell Genet43:103-8 1986 |
| PubMed ID: 3780313 |
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| McLellan T, Pryor MA, Kushner JP, Eddy RL, Shows TB, Assignment of uroporphyrinogen decarboxylase (UROD) to the pter----p21 region of human chromosome 1. Cytogenet Cell Genet39:224-7 1985 |
| PubMed ID: 4042691 |
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| Tricoli JV, Shows TB, Regional assignment of human amylase (AMY) to p22----p21 of chromosome 1. Somat Cell Mol Genet10:205-10 1984 |
| PubMed ID: 6608795 |
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| Benn PA, Shows TB, D'Ancona GG, Croce CM, Orkwiszewski KG, Mellman WJ, Assignment of a gene for uridine diphosphate galactose-4-epimerase to human chromosome 1 by somatic cell hybridization, with evidence for a regional assignment to 1pter yields 1p21. Cytogenet Cell Genet24:138-42 1979 |
| PubMed ID: 477411 |
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| Koch GA, Brown JA, Shows TB, Gene assignment of alpha-fucosidase and glucose dehydrogenase to the p21 leads to pter region of chromosome 1 in man. Somatic Cell Genet4:313-22 1978 |
| PubMed ID: 567853 |
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| Shows TB, Brown JA, Localization of the human uridine monophosphate kinase gene to the p21 leads to pter region of chromosome 1. Cytogenet Cell Genet16:227-30 1976 |
| PubMed ID: 185017 |
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| Shows TB, Brown JA, Mapping chromosomes 1 and 2 employing a 1/2 translocation in somatic cell hybrids. Cytogenet Cell Genet14:421-5 1975 |
| PubMed ID: 1192829 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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