GM14855
Somatic cell hybrid
Description:
CHROMOSOME 11 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 12 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Hybrids |
|
Cell Type
|
Somatic cell hybrid
|
|
Transformant
|
Untransformed
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
Human/mouse hybrid retaining a human der(11)t(11;12)(q23.3;p13) in 64% of cells and other human chromosomes
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| Remarks |
Line PBR-3; produced by fusing human bone marrow, t(11;12)(q23;p13), with HGPRT- RAG mouse cells; 64% of cells retain a human der(11)t(11;12)(q23.3; p13) and other human chromosomes |
| Chang ML, Eddy RL, Shows TB, Lau JT, Three genes that encode human beta-galactoside alpha 2,3- sialyltransferases. Structural analysis and chromosomal mapping studies. Glycobiology5:319-25 1995 |
| PubMed ID: 7655169 |
| |
| Polakiewicz RD, Munroe DJ, Sait SN, Tycowski KT, Nowak NJ, Shows TB, Housman DE, Page DC, Mapping of ribosomal protein S3 and internally nested snoRNA U15A gene to human chromosome 11q13.3-q13.5. Genomics25:577-80 1995 |
| PubMed ID: 7789996 |
| |
| Golden WL, von Kap-Herr C, Kurth B, Wright RM, Flickinger CJ, Eddy R, Shows T, Herr JC, Refinement of the localization of the gene for human intraacrosomal protein SP-10 (ACRV1) to the junction of bands q23-->q24 of chromosome 11 by nonisotopic in situ hybridization. Genomics18:446-9 1993 |
| PubMed ID: 8288254 |
| |
| Jani Sait SN, Raimondi SC, Look AT, Gill H, Thirman M, Diaz MO, Shows TB, A t(11;12) 11q23 leukemic breakpoint that disrupts the MLL gene. Genes Chromosomes Cancer7:28-31 1993 |
| PubMed ID: 7688552 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|
|