GM18157
LCL from B-Lymphocyte
Description:
INTERNATIONAL HAPMAP PROJECT - CHINESE IN METROPOLITAN DENVER, COLORADO, USA
INTERNATIONAL HAPMAP PROJECT - PANEL OF 90 CHINESE IN METROPOLITAN DENVER, COLORADO, USA
Repository
|
NHGRI Sample Repository for Human Genetic Research
|
Subcollection |
Human Variation |
Biopsy Source
|
Peripheral vein
|
Cell Type
|
B-Lymphocyte
|
Tissue Type
|
Blood
|
Transformant
|
Epstein-Barr Virus
|
Ethnicity
|
HAN CHINESE
|
Country of Origin
|
USA
|
Relation to Proband
|
proband
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
|
Remarks |
Han Chinese; sample collected from an individual living in the Denver, Colorado metropolitan area who is self-identified as having four Han Chinese grandparents |
Randolph AG, Lange C, Silverman EK, Lazarus R, Silverman ES, Raby B, Brown A, Ozonoff A, Richter B, Weiss ST, The IL12B gene is associated with asthma. Am J Hum Genet75(4):709-15 2004 |
PubMed ID: 15322986 |
Split Ratio |
1:5 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not Inactivated |
Substrate |
None specified |
Subcultivation Method |
dilution - add fresh medium |
|
|