GM25256
iPSC from Fibroblast
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently healthy iPSCs
Apparently Healthy Collection
PIGI Consented Sample |
| Protocols |
Protocol PDF |
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Biopsy Source
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Skin
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Cell Type
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Stem cell
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Tissue Type
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Induced pluripotent stem cell
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Transformant
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Reprogrammed (Episomal)
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Race
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Asian
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Hispanic Ethnicity
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Not Hispanic/Latino
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Ethnicity
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Japanese
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Country of Origin
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USA
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Family Member
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1
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Family History
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N
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis before cell line submission to CCR
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ISCN
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46,XY[19]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
39 |
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| Induced Pluripotent Stem Cell |
The frozen cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation and PluriTest. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis. |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by LINE assay |
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| Remark |
Induced pluripotent stem cell line derived from adult skin (leg) fibroblasts; subject clinically normal; wild-type; FISH test: 46, XY. This iPSC line (WTC-11) was submitted by Dr. Bruce R. Conklin (Gladstone Institute of Cardiovascular Disease, UCSF) and may serve as a control for diseased iPSCs GM25267 (LQT2) iPSC and GM25305 (LQT3); also available is a collection of fluorescently tagged hiPSC lines from the same subject, using CRISPR/Cas9 technology (for more information, see the Allen Cell Collection at Coriell: https://catalog.coriell.org/1/AllenCellCollection). Researchers purchasing hiPSCs from the NIGMS Repository are responsible for any limited use label licenses (LULLs) applicable to the cell line purchased. The applicable LULL to this line is iPS Academia, Japan Inc.. |
| Helms L, Marchiano S, Stanaway IB, Hsiang TY, Juliar BA, Saini S, Zhao YT, Khanna A, Menon R, Alakwaa F, Mikacenic C, Morrell ED, Wurfel MM, Kretzler M, Harder JL, Murry CE, Himmelfarb J, Ruohola-Baker H, Bhatraju PK, Gale M, Freedman BS, Cross-validation of SARS-CoV-2 responses in kidney organoids and clinical populations JCI insight6: 2021 |
| PubMed ID: 34767537 |
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| Muzzi L, Di Lisa D, Arnaldi P, Aprile D, Pastorino L, Martinoia S, Frega M, Rapid generation of functional engineered 3D human neuronal assemblies: network dynamics evaluated by micro-electrodes arrays Journal of neural engineering18: 2021 |
| PubMed ID: 34844234 |
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| Christopher GA, Noort RJ, Esseltine JL, Connexin 43 Gene Ablation Does Not Alter Human Pluripotent Stem Cell Germ Lineage Specification Biomolecules12: 2021 |
| PubMed ID: 35053163 |
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| He Z, Maynard A, Jain A, Gerber T, Petri R, Lin HC, Santel M, Ly K, Dupré JS, Sidow L, Sanchis Calleja F, Jansen SMJ, Riesenberg S, Camp JG, Treutlein B, Lineage recording in human cerebral organoids Nature methods19:90-99 2021 |
| PubMed ID: 34969984 |
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| Williams LM, Fujimoto T, Weaver RR, Logsdon AF, Evitts KM, Young JE, Banks WA, Erickson MA, Prolonged culturing of iPSC-derived brain endothelial-like cells is associated with quiescence, downregulation of glycolysis, and resistance to disruption by an Alzheimer's brain milieu Fluids and barriers of the CNS19:10 2021 |
| PubMed ID: 35123529 |
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| Song D, Zheng YW, Hemmi Y, An Y, Noguchi M, Nakamura Y, Oda T, Hayashi Y, Generation of human induced pluripotent stem cell lines carrying homozygous JAG1 deletions Stem cell research57:102588 2021 |
| PubMed ID: 34736037 |
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| Fort L, Gama V, Macara IG, Stem cell conversion to the cardiac lineage requires nucleotide signalling from apoptosing cells Nature cell biology24:434-447 2021 |
| PubMed ID: 35414019 |
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| Budjan C, Liu S, Ranga A, Gayen S, Pourquié O, Hormoz S, Paraxial mesoderm organoids model development of human somites eLife11:434-447 2021 |
| PubMed ID: 35088712 |
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| Charrez B, Charwat V, Siemons BA, Goswami I, Sakolish C, Luo YS, Finsberg H, Edwards AG, Miller EW, Rusyn I, Healy KE, Heart Muscle Microphysiological System for Cardiac Liability Prediction of Repurposed COVID-19 Therapeutics Frontiers in pharmacology12:684252 2021 |
| PubMed ID: 34421592 |
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| Pires Monteiro S, Voogd E, Muzzi L, De Vecchis G, Mossink B, Levers M, Hassink G, Van Putten M, Le Feber J, Hofmeijer J, Frega M, Neuroprotective effect of hypoxic preconditioning and neuronal activation in a in vitro human model of the ischemic penumbra Journal of neural engineering18:036016 2021 |
| PubMed ID: 33724235 |
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| Kuramochi Y, Awaya T, Matsuo-Takasaki M, Takami M, An Y, Li J, Hemmi Y, Wakabayashi T, Arai Y, Inoue J, Noguchi M, Nakamura Y, Asaka I, Akimoto K, Saito MK, Hayashi Y, Generation of two human induced pluripotent stem cell lines derived from two X-linked adrenoleukodystrophy patients with ABCD1 mutations Stem cell research53:102337 2021 |
| PubMed ID: 33901816 |
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| Zhang N, Bewick B, Schultz J, Tiwari A, Krencik R, Zhang A, Adachi K, Xia G, Yun K, Sarkar P, Ashizawa T, DNAzyme Cleavage of CAG Repeat RNA in Polyglutamine Diseases Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics53:102337 2021 |
| PubMed ID: 34160773 |
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| Notaras M, Lodhi A, Barrio-Alonso E, Foord C, Rodrick T, Jones D, Fang H, Greening D, Colak D, Neurodevelopmental signatures of narcotic and neuropsychiatric risk factors in 3D human-derived forebrain organoids Molecular psychiatry53:102337 2021 |
| PubMed ID: 34158620 |
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| Zinger A, Cvetkovic C, Sushnitha M, Naoi T, Baudo G, Anderson M, Shetty A, Basu N, Covello J, Tasciotti E, Amit M, Xie T, Taraballi F, Krencik R, Humanized Biomimetic Nanovesicles for Neuron Targeting Advanced science (Weinheim, Baden-Wurttemberg, Germany)53:e2101437 2021 |
| PubMed ID: 34382379 |
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| Aisenbrey EA, Torr E, Johnson H, Soref C, Daly W, Murphy WL, A protocol for rapid pericyte differentiation of human induced pluripotent stem cells STAR protocols2:100261 2021 |
| PubMed ID: 33490977 |
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| Middelkamp HHT, Verboven AHA, De Sá Vivas AG, Schoenmaker C, Klein Gunnewiek TM, Passier R, Albers CA, 't Hoen PAC, Nadif Kasri N, van der Meer AD, Cell type-specific changes in transcriptomic profiles of endothelial cells, iPSC-derived neurons and astrocytes cultured on microfluidic chips Scientific reports11:2281 2020 |
| PubMed ID: 33500551 |
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| Jæger KH, Charwat V, Wall S, Healy KE, Tveito A, Identifying Drug Response by Combining Measurements of the Membrane Potential, the Cytosolic Calcium Concentration, and the Extracellular Potential in Microphysiological Systems Frontiers in pharmacology11:569489 2020 |
| PubMed ID: 33628168 |
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| Ortolano NA, Romero-Morales AI, Rasmussen ML, Bodnya C, Kline LA, Joshi P, Connelly JP, Rose KL, Pruett-Miller SM, Gama V, A proteomics approach for the identification of cullin-9 (CUL9) related signaling pathways in induced pluripotent stem cell models PloS one16:e0248000 2020 |
| PubMed ID: 33705438 |
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| Long Y, Hwang T, Gooding AR, Goodrich KJ, Rinn JL, Cech TR, RNA is essential for PRC2 chromatin occupancy and function in human pluripotent stem cells Nature genetics16:e0248000 2020 |
| PubMed ID: 32632336 |
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| Arai Y, Takami M, An Y, Matsuo-Takasaki M, Hemmi Y, Wakabayashi T, Inoue J, Noguchi M, Nakamura Y, Sugimoto K, Takemura T, Okita K, Osafune K, Takasato M, Hayata T, Hayashi Y, Generation of two human induced pluripotent stem cell lines derived from two juvenile nephronophthisis patients with NPHP1 deletion Stem cell research45:101815 2020 |
| PubMed ID: 32361464 |
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| Liu LP, Guo NN, Li YM, Zheng YW, Generation of Human iMelanocytes from Induced Pluripotent Stem Cells through a Suspension Culture System STAR protocols1:100004 2020 |
| PubMed ID: 33111066 |
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| Zampeta IF, Sonzogni M, Niggl E, Lendemeijer B, Smeenk H, de Vrij FMS, Kushner SA, Distel B, Elgersma Y, Conserved UBE3A subcellular distribution between human and mice is facilitated by non-homologous isoforms Human molecular genetics1:100004 2020 |
| PubMed ID: 32879944 |
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| Youmans DT, Gooding AR, Dowell RD, Cech TR, Competition between PRC21 and 22 subcomplexes regulates PRC2 chromatin occupancy in human stem cells Molecular cell1:100004 2020 |
| PubMed ID: 33338397 |
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| Libby ARG, Joy DA, McDevitt TC, Engineering the Spatiotemporal Mosaic Self-Patterning of Pluripotent Stem Cells Methods in molecular biology (Clifton, NJ)2258:105-116 2020 |
| PubMed ID: 33340357 |
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| Tsan YC, DePalma SJ, Zhao YT, Capilnasiu A, Wu YW, Elder B, Panse I, Ufford K, Matera DL, Friedline S, O'Leary TS, Wubshet N, Ho KKY, Previs MJ, Nordsletten D, Isom LL, Baker BM, Liu AP, Helms AS, Physiologic biomechanics enhance reproducible contractile development in a stem cell derived cardiac muscle platform Nature communications12:6167 2020 |
| PubMed ID: 34697315 |
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| Long Y, Cech TR, Targeted mutagenesis in human iPSCs using CRISPR genome-editing tools Methods (San Diego, Calif)191:44-58 2020 |
| PubMed ID: 33444739 |
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| Li L, Li Y, Sottas C, Culty M, Fan J, Hu Y, Cheung G, Chemes HE, Papadopoulos V, Directing differentiation of human induced pluripotent stem cells toward androgen-producing Leydig cells rather than adrenal cells Proceedings of the National Academy of Sciences of the United States of America191:44-58 2019 |
| PubMed ID: 31591190 |
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| Libby ARG, Briers D, Haghighi I, Joy DA, Conklin BR, Belta C, McDevitt TC, Automated Design of Pluripotent Stem Cell Self-Organization Cell systems9:483-495.e10 2019 |
| PubMed ID: 31759947 |
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| Schneider O, Zeifang L, Fuchs S, Sailer C, Loskill P, User-Friendly and Parallelized Generation of Human Induced Pluripotent Stem Cell-Derived Microtissues in a Centrifugal Heart-on-a-Chip Tissue engineering Part A25:786-798 2019 |
| PubMed ID: 30968738 |
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| Madsen RR, Semple RK, Luminescent peptide tagging enables efficient screening for CRISPR-mediated knock-in in human induced pluripotent stem cells Wellcome open research4:37 2019 |
| PubMed ID: 31363496 |
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| Regier MC, Tokar JJ, Warrick JW, Pabon L, Berthier E, Beebe DJ, Stevens KR, User-defined morphogen patterning for directing human cell fate stratification Scientific reports9:6433 2019 |
| PubMed ID: 31015521 |
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| Kim E, Wu F, Wu X, Choo HJ, Generation of craniofacial myogenic progenitor cells from human induced pluripotent stem cells for skeletal muscle tissue regeneration Biomaterials248:119995 2019 |
| PubMed ID: 32283390 |
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| Nickolls AR, Lee MM, Espinoza DF, Szczot M, Lam RM, Wang Q, Beers J, Zou J, Nguyen MQ, Solinski HJ, AlJanahi AA, Johnson KR, Ward ME, Chesler AT, Bönnemann CG, Transcriptional Programming of Human Mechanosensory Neuron Subtypes from Pluripotent Stem Cells Cell reports30:932-946.e7 2019 |
| PubMed ID: 31968264 |
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| Guo X, Aviles G, Liu Y, Tian R, Unger BA, Lin YT, Wiita AP, Xu K, Correia MA, Kampmann M, Mitochondrial stress is relayed to the cytosol by an OMA1-DELE1-HRI pathway Nature579:427-432 2019 |
| PubMed ID: 32132707 |
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| Wilson E, Rudisill T, Kirk B, Johnson C, Kemper P, Newell-Litwa K, Cytoskeletal regulation of synaptogenesis in a model of human fetal brain development Journal of neuroscience research579:427-432 2019 |
| PubMed ID: 32713041 |
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| Gong S, Li Y, Su W, Ding Y, Lu J, Dong K, Hood S, Zhang W, Terstappen GC, Quantitative Algorithm-Based Paired Imaging Measurement for Antibody-Triggered Endocytosis in Cultured Cells SLAS discovery : advancing life sciences R & D579:2472555218761355 2018 |
| PubMed ID: 29505735 |
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| Hoang P, Wang J, Conklin BR, Healy KE, Ma Z, Generation of spatial-patterned early-developing cardiac organoids using human pluripotent stem cells Nature protocols13:723-737 2018 |
| PubMed ID: 29543795 |
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| Li Y, Sun X, Liu H, Huang L, Meng G, Ding Y, Su W, Lu J, Gong S, Terstappen GC, Zhang R, Zhang W, Development of Human in vitro Brain-blood Barrier Model from Induced Pluripotent Stem Cell-derived Endothelial Cells to Predict the in vivo Permeability of Drugs Neuroscience bulletin35:996-1010 2018 |
| PubMed ID: 31079318 |
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| Bertero A, Fields PA, Ramani V, Bonora G, Yardimci GG, Reinecke H, Pabon L, Noble WS, Shendure J, Murry CE, Dynamics of genome reorganization during human cardiogenesis reveal an RBM20-dependent splicing factory Nature communications10:1538 2018 |
| PubMed ID: 30948719 |
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| Libby AR, Joy DA, So PL, Mandegar MA, Muncie JM, Mendoza-Camacho FN, Weaver VM, Conklin BR, McDevitt TC, Spatiotemporal mosaic self-patterning of pluripotent stem cells using CRISPR interference Elife10:1538 2018 |
| PubMed ID: 30298816 |
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| Miklas JW, Clark E, Levy S, Detraux D, Leonard A, Beussman K, Showalter MR, Smith AT, Hofsteen P, Yang X, Macadangdang J, Manninen T, Raftery D, Madan A, Suomalainen A, Kim DH, Murry CE, Fiehn O, Sniadecki NJ, Wang Y, Ruohola-Baker H, TFPa/HADHA is required for fatty acid beta-oxidation and cardiolipin re-modeling in human cardiomyocytes Nature communications10:4671 2018 |
| PubMed ID: 31604922 |
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| Colasuonno F, Borghi R, Niceforo A, Muzzi M, Bertini E, Di Giulio A, Moreno S, Compagnucci C, Senescence-associated ultrastructural features of long-term cultures of induced pluripotent stem cells (iPSCs) Aging10:4671 2017 |
| PubMed ID: 29064821 |
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| Barber K, Studer L, Fattahi F, Derivation of enteric neuron lineages from human pluripotent stem cells Nature protocols14:1261-1279 2016 |
| PubMed ID: 30911172 |
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| Huebsch N, Loskill P, Mandegar MA, Marks NC, Sheehan AS, Ma Z, Mathur A, Nguyen TN, Yoo JC, Judge LM, Spencer CI, Chukka AC, Russell CR, So PL, Conklin BR, Healy KE, Automated Video-Based Analysis of Contractility and Calcium Flux in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Cultured over Different Spatial Scales Tissue engineering Part C, Methods21:467-79 2015 |
| PubMed ID: 25333967 |
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| Miyaoka Y, Chan AH, Judge LM, Yoo J, Huang M, Nguyen TD, Lizarraga PP, So PL, Conklin BR, Isolation of single-base genome-edited human iPS cells without antibiotic selection Nature methods11:291-3 2013 |
| PubMed ID: 24509632 |
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| Kreitzer FR, Salomonis N, Sheehan A, Huang M, Park JS, Spindler MJ, Lizarraga P, Weiss WA, So PL, Conklin BR, A robust method to derive functional neural crest cells from human pluripotent stem cells American journal of stem cells2:119-31 2013 |
| PubMed ID: 23862100 |
| Passage Frozen |
39 |
| Split Ratio |
1:9 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
mTeSR1 |
| Serum |
none |
| Substrate |
Matrigel |
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