Repository NIA Aging Cell Culture Repository
Subcollection IMR90/IMR91 Cell Cultures
Specially Characterized Fibroblasts
Biopsy Source Lung
Cell Type Fibroblast
Tissue Type Lung
Transformant Untransformed
Race Caucasian
Relation to Proband proband
Confirmation Clinical summary/Case history
Species Homo sapiens
Common Name Human
Remarks The IMR-90 line was established on 7/7/75 using explants of minced lung tissue obtained from a clinically normal 16 week fetus. The karyotype is 46,XX; normal diploid female. No variant isoenzyme patterns found for lactate dehydrogenase, malate dehydrogenase, 6-phosphogluconate dehydrogenase, and glucose-6-phosphate dehydrogenase; G6PD is isoenzyme type B. The HLA type is A2, B12, BW40, B5. Reverse transcriptase was not detected. The maximum number of population doublings achieved to senescence range from 58-73. Earlier passage IMR-90 cells available only with permission of NIA: see guidelines in Science 196:60-63 (1977).
PDL at Senescence 62
PDL at Freeze 19
Passage Frozen 5
 
Remark The IMR-90 line was established on 7/7/75 using explants of minced lung tissue obtained from a clinically normal 16 week fetus. The karyotype is 46,XX; normal diploid female. No variant isoenzyme patterns found for lactate dehydrogenase, malate dehydrogenase, 6-phosphogluconate dehydrogenase, and glucose-6-phosphate dehydrogenase; G6PD is isoenzyme type B. The HLA type is A2, B12, BW40, B5. Reverse transcriptase was not detected. The maximum number of population doublings achieved to senescence range from 58-73. Earlier passage IMR-90 cells available only with permission of NIA: see guidelines in Science 196:60-63 (1977).
Vallot C, Huret C, Lesecque Y, Resch A, Oudrhiri N, Bennaceur-Griscelli A, Duret L, Rougeulle C, XACT, a long noncoding transcript coating the active X chromosome in human pluripotent cells Nat Genet45(3):239-41 2013
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Sanders YY, Liu H, Zhang X, Hecker L, Hernard K, Desai L, Liu G, Thannickal VJ, Histone modifications in senescence-associated resistance to apoptosis by oxidative stress Redox Biol1(1):8-16 2013
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Mao Z, Ke Z, Gobunova V, Seluanov A., Replicatively senescent cells are arrested in G1 and G2 phases. Aging4:431-5 2012
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Cho JH, Saini DK, Karunarathne WK, Kalyanaraman V, Gautam N., Alteration of Golgi structure in senescent cells and its regulation by a G protein γ subunit. Cell Signal23:785-93 2011
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Ivanov VN, Zhou H, Ghandhi SA, Karasic TB, Yaghoubian B, Amundson SA, Hei TK, Radiation-induced bystander signaling pathways in human fibroblasts: a role for interleukin-33 in the signal transmission Cellular signalling22:1076-87 2010
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Ghandhi SA, Ming L, Ivanov VN, Hei TK, Amundson SA, Regulation of early signaling and gene expression in the alpha-particle and bystander response of IMR-90 human fibroblasts BMC medical genomics3:31 2010
PubMed ID: 20670442
 
Eymery A, Souchier C, Vourc'h C, Jolly C, Heat shock factor 1 binds to and transcribes satellite II and III sequences at several pericentromeric regions in heat-shocked cells Experimental cell research316:1845-55 2009
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Gamerdinger M, Clement AB, Behl C, Cholesterol-Like Effects of Selective Cyclooxygenase Inhibitors and Fibrates on Cellular Membranes and Amyloid-beta Production Molecular pharmacology72:141-51 2007
PubMed ID: 17395689
 
Milam JE, Keshamouni VG, Phan SH, Hu B, Gangireddy SR, Hogaboam CM, Standiford TJ, Thannickal VJ, Reddy RC, PPAR-gamma agonists inhibit profibrotic phenotypes in human lung fibroblasts and bleomycin-induced pulmonary fibrosis American journal of physiology Lung cellular and molecular physiology294:L891-901 2007
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Atamna H, Newberry J, Erlitzki R, Schultz CS, Ames BN, Biotin deficiency inhibits heme synthesis and impairs mitochondria in human lung fibroblasts The Journal of nutrition137:25-30 2006
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Gosslau A, Chen M, Ho CT, Chen KY, A methoxy derivative of resveratrol analogue selectively induced activation of the mitochondrial apoptotic pathway in transformed fibroblasts Br J Cancer92(3):513-21 2005
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Yin Z, Watsky MA, Chloride channel activity in human lung fibroblasts and myofibroblasts American journal of physiology Lung cellular and molecular physiology288:L1110-6 2005
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White ES, Atrasz RG, Hu B, Phan SH, Stambolic V, Mak TW, Hogaboam CM, Flaherty KR, Martinez FJ, Kontos CD, Toews GB, Negative regulation of myofibroblast differentiation by PTEN (Phosphatase and Tensin Homolog Deleted on chromosome 10) American journal of respiratory and critical care medicine173:112-21 2005
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Kern A, Roempp B, Prager K, Walter J, Behl C, Down-regulation of endogenous amyloid precursor protein processing due to cellular aging The Journal of biological chemistry281:2405-13 2005
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Kim TH, Barrera LO, Qu C, Van Calcar S, Trinklein ND, Cooper SJ, Luna RM, Glass CK, Rosenfeld MG, Myers RM, Ren B, Direct isolation and identification of promoters in the human genome Genome research15:830-9 2005
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White ES, Atrasz RG, Dickie EG, Aronoff DM, Stambolic V, Mak TW, Moore BB, Peters-Golden M, Prostaglandin E(2) inhibits fibroblast migration by E-prostanoid 2 receptor-mediated increase in PTEN activity American journal of respiratory cell and molecular biology32:135-41 2004
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Silverman J, Takai H, Buonomo SB, Eisenhaber F, de Lange T, Human Rif1, ortholog of a yeast telomeric protein, is regulated by ATM and 53BP1 and functions in the S-phase checkpoint. Genes Dev18(17):2108-19 2004
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Yang J, Xu ZP, Huang Y, Hamrick HE, Duerksen-Hughes PJ, Yu YN, ATM and ATR: sensing DNA damage. World J Gastroenterol10(2):155-60 2004
PubMed ID: 14716813
 
Li GZ, Eller MS, Firoozabadi R, Gilchrest BA, Evidence that exposure of the telomere 3' overhang sequence induces senescence Proceedings of the National Academy of Sciences of the United States of America100:527-31 2003
PubMed ID: 12515865
 
Forsyth NR, Evans AP, Shay JW, Wright WE, Developmental differences in the immortalization of lung fibroblasts by telomerase. Aging Cell2(5):235-43 2003
PubMed ID: 14570231
 
Thannickal VJ, Lee DY, White ES, Cui Z, Larios JM, Chacon R, Horowitz JC, Day RM, Thomas PE, Myofibroblast differentiation by transforming growth factor-beta1 is dependent on cell adhesion and integrin signaling via focal adhesion kinase The Journal of biological chemistry278:12384-9 2003
PubMed ID: 12531888
 
Parada C, Hernandez Losa J, Guinea J, Sanchez-Arevalo V, Fernandez Soria V, Alvarez-Vallina L, Sanchez-Prieto R, Ramon Y Cajal S, Adenovirus E1a protein enhances the cytotoxic effects of the herpes thymidine kinase-ganciclovir system. Cancer Gene Ther10(2):152-60 2003
PubMed ID: 12536204
 
Baird DM, Rowson J, Wynford-Thomas D, Kipling D, Extensive allelic variation and ultrashort telomeres in senescent human cells. Nat Genet33(2):203-7 2003
PubMed ID: 12539050
 
Deddish PA, Marcic BM, Tan F, Jackman HL, Chen Z, Erdos EG, Neprilysin inhibitors potentiate effects of bradykinin on b2 receptor. Hypertension39(2 Pt 2):619-23 2002
PubMed ID: 11882619
 
Gabai VL, Mabuchi K, Mosser DD, Sherman MY, Hsp72 and stress kinase c-jun N-terminal kinase regulate the bid-dependent pathway in tumor necrosis factor-induced apoptosis. Mol Cell Biol22(10):3415-24 2002
PubMed ID: 11971973
 
Zhang X, Shu L, Hosoi H, Murti KG, Houghton PJ, Predominant nuclear localization of mammalian target of rapamycin in normal and malignant cells in culture. J Biol Chem277(31):28127-34 2002
PubMed ID: 12000755
 
Shreeram S, Sparks A, Lane DP, Blow JJ, Cell type-specific responses of human cells to inhibition of replication licensing. Oncogene21(43):6624-32 2002
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Yazdi PT, Wang Y, Zhao S, Patel N, Lee EY, Qin J, SMC1 is a downstream effector in the ATM/NBS1 branch of the human S-phase checkpoint. Genes Dev16(5):571-82 2002
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Borel F, Lohez OD, Lacroix FB, Margolis RL, Multiple centrosomes arise from tetraploidy checkpoint failure and mitotic centrosome clusters in p53 and RB pocket protein-compromised cells Proc Natl Acad Sci U S A99(15):9819-24 2002
PubMed ID: 12119403
 
Tang D, Wu D, Hirao A, Lahti JM, Liu L, Mazza B, Kidd VJ, Mak TW, Ingram AJ, ERK activation mediates cell cycle arrest and apoptosis after DNA damage independently of p53. J Biol Chemepub ahead of print:9819-24 2002
PubMed ID: 11821415
 
Danam RP, Howell SR, Remack JS, Brent TP, Heterogeneous methylation of the O(6)-methylguanine-DNA methyltransferase promoter in immortalized IMR90 cell lines. Int J Oncol18(6):1187-93 2001
PubMed ID: 11351250
 
Yang X, Taylor L, Polgar P, p53 down-regulates human bradykinin B1 receptor gene expression. J Cell Biochem82(1):38-45 2001
PubMed ID: 11400161
 
Yang X, Taylor L, Yu J, Fenton MJ, Polgar P, Mediator caused induction of a human bradykinin B1 receptor minigene: participation of c-Jun in the process. J Cell Biochem82(1):163-70 2001
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Guo W, Okamoto M, Park NH, Lee YM, Park NH, Cloning and expression of hamster telomerase catalytic subunit cDNA. Int J Mol Med8(1):73-8 2001
PubMed ID: 11408953
 
Atamna H, Liu J, Ames BN, Heme deficiency selectively interrupts assembly of mitochondrial complex IV in human fibroblasts: revelance to aging. J Biol Chem276(51):48410-6 2001
PubMed ID: 11598132
 
Atamna H, Robinson C, Ingersoll R, Elliott H, Ames BN, N-t-Butyl hydroxylamine is an antioxidant that reverses age-related changes in mitochondria in vivo and in vitro. FASEB J15(12):2196-204 2001
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Andreassen PR, Lohez OD, Lacroix FB, Margolis RL, Tetraploid state induces p53-dependent arrest of nontransformed mammalian cells in G1. Mol Biol Cell12(5):1315-28 2001
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Atamna H, Cheung I, Ames BN, A method for detecting abasic sites in living cells: age-dependent changes in base excision repair. Proc Natl Acad Sci U S A97(2):686-91 2000
PubMed ID: 10639140
 
Yang X, He Z, Xin B, Cao L, LMP1 of Epstein-Barr virus suppresses cellular senescence associated with the inhibition of p16INK4a expression Oncogene19(16):2002-13 2000
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Ferbeyre G, de Stanchina E, Querido E, Baptiste N, Prives C, Lowe SW, PML is induced by oncogenic ras and promotes premature senescence. Genes Dev14(16):2015-27 2000
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Atamna H, Paler-Martinez A, Ames BN, N-t-butyl hydroxylamine, a hydrolysis product of alpha-phenyl-N-t-butyl nitrone, is more potent in delaying senescence in human lung fibroblasts. J Biol Chem275:6741-8 2000
PubMed ID: 10702229
 
Martelli F, Livingston DM, Regulation of endogenous E2F1 stability by the retinoblastoma family proteins. Proc Natl Acad Sci U S A96:2858-63 1999
PubMed ID: 10077601
 
Blackburn RV, Spitz DR, Liu X, Galoforo SS, Sim JE, Ridnour LA, Chen JC, Davis BH, Corry PM, Lee YJ, Metabolic oxidative stress activates signal transduction and gene expression during glucose deprivation in human tumor cells. Free Radic Biol Med26(3-4):419-30 1999
PubMed ID: 9895234
 
Ouellette MM, Aisner DL, Savre-Train I, Wright WE, Shay JW, Telomerase activity does not always imply telomere maintenance. Biochem Biophys Res Commun254(3):795-803 1999
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Hay JG, Shapiro N, Sauthoff H, Heitner S, Phupakdi W, Rom WN, Targeting the replication of adenoviral gene therapy vectors to lung cancer cells: the importance of the adenoviral E1b-55kD gene. Hum Gene Ther10(4):579-90 1999
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Rudolph C, Adam G, Simm A, Determination of copy number of c-Myc protein per cell by quantitative Western blotting. Anal Biochem269(1):66-71 1999
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Chiou WJ, Wang J, Berg CE, Wu-Wong JR, SV40 virus transformation down-regulates endothelin receptor. Biochim Biophys Acta1450(1):35-44 1999
PubMed ID: 10231553
 
Suzuki K, Kazui T, Yoshida M, Uno T, Kobayashi T, Kimura T, Yoshida T, Sugimura H, Drug-induced apoptosis and p53, BCL-2 and BAX expression in breast cancer tissues in vivo and in fibroblast cells in vitro. Jpn J Clin Oncol29(7):323-31 1999
PubMed ID: 10470656
 
Karlseder J, Broccoli D, Dai Y, Hardy S, de Lange T, p53- and ATM-dependent apoptosis induced by telomeres lacking TRF2. Science283(5406):1321-5 1999
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Zhou X, Polgar P, Taylor L, Roles for interleukin-1beta, phorbol ester and a post-transcriptional regulator in the control of bradykinin B1 receptor gene expression Biochem J330 ( Pt 1):361-6 1998
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Yang X, Taylor L, Polgar P, Mechanisms in the transcriptional regulation of bradykinin B1 receptor gene expression. Identification of a minimum cell-type specific enhancer. J Biol Chem273(17):10763-70 1998
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Lukiw WJ, Bazan NG, Strong nuclear factor-kappaB-DNA binding parallels cyclooxygenase-2 gene transcription in aging and in sporadic Alzheimer's disease superior temporal lobe neocortex. J Neurosci Res53(5):583-92 1998
PubMed ID: 9726429
 
Stein GH, Dulic V, Molecular mechanisms for the senescent cell cycle arrest. J Investig Dermatol Symp Proc3(1):14-8 1998
PubMed ID: 9732051
 
Choung J, Taylor L, Thomas K, Zhou X, Kagan H, Yang X, Polgar P, Role of EP2 receptors and cAMP in prostaglandin E2 regulated expression of type I collagen alpha1, lysyl oxidase, and cyclooxygenase-1 genes in human embryo lung fibroblasts. J Cell Biochem71(2):254-63 1998
PubMed ID: 9779823
 
Jiang WQ, Ringertz N, Altered distribution of the promyelocytic leukemia-associated protein is associated with cellular senescence. Cell Growth Differ8(5):513-22 1997
PubMed ID: 9213441
 
Baldin V, Cans C, Watanabe N, Ducommun B, Evidence for a mammalian Nim1-like kinase pathway acting at the G0-1/S transition. Biochem Biophys Res Commun236(1):130-4 1997
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Inamura N, Asano M, Kayakiri H, Hatori C, Oku T, Nakahara K, Characterization of FR173657, a novel nonpeptide B2 antagonist: in vitro and in vivo studies. Can J Physiol Pharmacol75(6):622-8 1997
PubMed ID: 9276139
 
Wang X, Gorospe M, Huang Y, Holbrook NJ, p27Kip1 overexpression causes apoptotic death of mammalian cells. Oncogene15(24):2991-7 1997
PubMed ID: 9416843
 
Makarov VL, Hirose Y, Langmore JP, Long G tails at both ends of human chromosomes suggest a C strand degradation mechanism for telomere shortening. Cell88:657-66 1997
PubMed ID: 9054505
 
Krupsky M, Kuang PP, Goldstein RH, Regulation of type I collagen mRNA by amino acid deprivation in human lung fibroblasts. J Biol Chem272:13864-8 1997
PubMed ID: 9153245
 
Rodriguez A, Webster P, Ortego J, Andrews NW, Lysosomes behave as Ca2+-regulated exocytic vesicles in fibroblasts and epithelial cells. J Cell Biol137(1):93-104 1997
PubMed ID: 9105039
 
Saito H, Papaconstantinou J, Sato H, Goldstein S, Regulation of a novel gene encoding a lysyl oxidase-related protein in cellular adhesion and senescence. J Biol Chem272:8157-60 1997
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Harris LC, Remack JS, Houghton PJ, Brent TP, Wild-type p53 suppresses transcription of the human O6-methylguanine-DNA methyltransferase gene. Cancer Res56(9):2029-32 1996
PubMed ID: 8616846
 
Harris LC, von Wronski MA, Venable CC, Remack JS, Howell SR, Brent TP, Changes in O6-methylguanine-DNA methyltransferase expression during immortalization of cloned human fibroblasts. Carcinogenesis17(2):219-24 1996
PubMed ID: 8625442
 
Harrison L, Galanopoulos T, Ascione AG, Antoniades HN, Demple B, Regulated expression of APE apurinic endonuclease mRNA during wound healing in porcine epidermis. Carcinogenesis17(2):377-81 1996
PubMed ID: 8625467
 
Roy R, Polgar P, Wang Y, Goldstein RH, Taylor L, Kagan HM, Regulation of lysyl oxidase and cyclooxygenase expression in human lung fibroblasts: interactions among TGF-beta, IL-1 beta, and prostaglandin E. J Cell Biochem62(3):411-7 1996
PubMed ID: 8872612
 
Dalemar LR, Ivy Jong YJ, Wilhelm B, Baenziger NL, Protein kinases A and C rapidly modulate expression of human lung fibroblast B2 bradykinin receptor affinity forms. Eur J Cell Biol69(3):236-44 1996
PubMed ID: 8900488
 
Baldin V, Ducommun B, Subcellular localisation of human wee1 kinase is regulated during the cell cycle. J Cell Sci108 ( Pt 6):2425-32 1995
PubMed ID: 7673359
 
Saito H, Hammond AT, Moses RE, The effect of low oxygen tension on the in vitro-replicative life span of human diploid fibroblast cells and their transformed derivatives. Exp Cell Res217(2):272-9 1995
PubMed ID: 7698226
 
Halle JP, Schmidt C, Adam G, Changes of the methylation pattern of the c-myc gene during in vitro aging of IMR90 human embryonic fibroblasts. Mutat Res316(4):157-71 1995
PubMed ID: 7776985
 
Hendil KB, Kristensen P, Uerkvitz W, Human proteasomes analysed with monoclonal antibodies. Biochem J305 ( Pt 1):245-52 1995
PubMed ID: 7826336
 
Jesudasan RA, Rahman RA, Chandrashekharappa S, Evans GA, Srivatsan ES, Deletion and translocation of chromosome 11q13 sequences in cervical carcinoma cell lines [see comments] Am J Hum Genet56:705-15 1995
PubMed ID: 7887426
 
Puglielli L, Rigotti A, Greco AV, Santos MJ, Nervi F, Sterol carrier protein-2 is involved in cholesterol transfer from the endoplasmic reticulum to the plasma membrane in human fibroblasts. J Biol Chem270:18723-6 1995
PubMed ID: 7642518
 
Borsi L, Balza E, Gaggero B, Allemanni G, Zardi L, The alternative splicing pattern of the tenascin-C pre-mRNA is controlled by the extracellular pH. J Biol Chem270:6243-5 1995
PubMed ID: 7534307
 
Duan C, Clemmons DR, Transcription factor AP-2 regulates human insulin-like growth factor binding protein-5 gene expression. J Biol Chem270:24844-51 1995
PubMed ID: 7559606
 
Sagesaka T, Boubnov N, Okuyama T, Paulus H, Sarkar N, Deoxyribonucleic acid replication in fetal cells. Am J Obstet Gynecol170(2):468-73 1994
PubMed ID: 8116699
 
Chang ZF, Huang DY, Decline of protein kinase C activation in response to growth stimulation during senescence of IMR-90 human diploid fibroblasts. Biochem Biophys Res Commun200:16-27 1994
PubMed ID: 8166683
 
Rubelj I, Pereira-Smith OM, SV40-transformed human cells in crisis exhibit changes that occur in normal cellular senescence. Exp Cell Res211:82-9 1994
PubMed ID: 7510251
 
Saito H, Grompe M, Neeley TL, Jakobs PM, Moses RE, Fanconi anemia cells have a normal gene structure for topoisomerase I. Hum Genet93:583-6 1994
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Vannini F, Meacci E, Vasta V, Farnararo M, Bruni P, Involvement of protein kinase C and arachidonate signaling pathways in the alteration of proliferative response of senescent IMR-90 human fibroblasts. Mech Ageing Dev76:101-11 1994
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Li Y, Jenkins CW, Nichols MA, Xiong Y, Cell cycle expression and p53 regulation of the cyclin-dependent kinase inhibitor p21. Oncogene9(8):2261-8 1994
PubMed ID: 7913544
 
Mannironi C, Orr A, Hatch C, Pilch D, Ivanova V, Bonner W, The relative expression of human histone H2A genes is similar in different types of proliferating cells. DNA Cell Biol13(2):161-70 1994
PubMed ID: 8179821
 
Jackson BA, Goldstein RH, Roy R, Cozzani M, Taylor L, Polgar P, Effects of transforming growth factor beta and interleukin-1 beta on expression of cyclooxygenase 1 and 2 and phospholipase A2 mRNA in lung fibroblasts and endothelial cells in culture. Biochem Biophys Res Commun197(3):1465-74 1993
PubMed ID: 8280164
 
Manfioletti G, Brancolini C, Avanzi G, Schneider C, The protein encoded by a growth arrest-specific gene (gas6) is a new member of the vitamin K-dependent proteins related to protein S, a negative coregulator in the blood coagulation cascade. Mol Cell Biol13(8):4976-85 1993
PubMed ID: 8336730
 
Wu C, Zannis-Hadjopoulos M, Price GB, In vivo activity for initiation of DNA replication resides in a transcribed region of the human genome. Biochim Biophys Acta1174(3):258-66 1993
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Kelley J, Shull S, Walsh JJ, Cutroneo KR, Absher M, Auto-induction of transforming growth factor-beta in human lung fibroblasts. Am J Respir Cell Mol Biol8(4):417-24 1993
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Wu C, Friedlander P, Lamoureux C, Zannis-Hadjopoulos M, Price GB, cDNA clones contain autonomous replication activity. Biochim Biophys Acta1174(3):241-57 1993
PubMed ID: 7690594
 
Chang ZF, Cheng SM, Constitutive overexpression of DNA binding activity to the distal CCAAT box of human thymidine kinase promoter in human tumor cell lines. Cancer Res53:3253-6 1993
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Schaefer DI, Livanos EM, White AE, Tlsty TD, Multiple mechanisms of N-(phosphonoacetyl)-L-aspartate drug resistance in SV40-infected precrisis human fibroblasts. Cancer Res53:4946-51 1993
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Sakai T, Satoh K, Narumi K, Abe T, Motomiya M, Cloning of 67 kDa-laminin receptor cDNA and its expression in various human lung cancer cell lines. Nihon Kyobu Shikkan Gakkai Zasshi30(3):402-6 1992
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Baenziger NL, Jong YJ, Yocum SA, Dalemar LR, Wilhelm B, Vavrek R, Stewart JM, Diversity of B2 bradykinin receptors with nanomolar affinity expressed in passaged IMR90 human lung fibroblasts. Eur J Cell Biol58(1):71-80 1992
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Satoh K, Narumi K, Sakai T, Abe T, Kikuchi T, Matsushima K, Sindoh S, Motomiya M, Cloning of 67-kDa laminin receptor cDNA and gene expression in normal and malignant cell lines of the human lung. Cancer Lett62(3):199-203 1992
PubMed ID: 1534510
 
Yang CL, Chang LS, Zhang P, Hao H, Zhu L, Toomey NL, Lee MY, Molecular cloning of the cDNA for the catalytic subunit of human DNA polymerase delta. Nucleic Acids Res20(4):735-45 1992
PubMed ID: 1542570
 
Fukunaga N, Burrows HL, Meyers M, Schea RA, Boothman DA, Enhanced induction of tissue-type plasminogen activator in normal human cells compared to cancer-prone cells following ionizing radiation. Int J Radiat Oncol Biol Phys24(5):949-57 1992
PubMed ID: 1447036
 
Paw BH, Wood LC, Neufeld EF, A third mutation at the CpG dinucleotide of codon 504 and a silent mutation at codon 506 of the HEX A gene. Am J Hum Genet48:1139-46 1991
PubMed ID: 1827944
 
Wu CH, Walton CM, Wu GY, Propeptide-mediated regulation of procollagen synthesis in IMR-90 human lung fibroblast cell cultures. Evidence for transcriptional control. J Biol Chem266:2983-7 1991
PubMed ID: 1993671
 
Magnuson VL, Young M, Schattenberg DG, Mancini MA, Chen DL, Steffensen B, Klebe RJ, The alternative splicing of fibronectin pre-mRNA is altered during aging and in response to growth factors. J Biol Chem266:14654-62 1991
PubMed ID: 1713586
 
Sun Y, Moses RE, Reactivation of psoralen-reacted plasmid DNA in Fanconi anemia, xeroderma pigmentosum, and normal human fibroblast cells. Somat Cell Mol Genet17:229-38 1991
PubMed ID: 2047939
 
Solem M, Helmrich A, Collodi P, Barnes D, Human and mouse S-protein mRNA detected in northern blot experiments and evidence for the gene encoding S-protein in mammals by Southern blot analysis. Mol Cell Biochem100(2):141-9 1991
PubMed ID: 2008176
 
Shigeoka H, Yang HC, Regulation of the retinoblastoma anti-oncogene in aging human diploid fibroblasts. Mech Ageing Dev57:63-70 1991
PubMed ID: 2002699
 
Saito H, Moses RE, Immortalization of Werner syndrome and progeria fibroblasts Experimental cell research192:373-9 1991
PubMed ID: 1671011
 
Vlahos NS, Futscher BW, Hora NK, Trent JM, Erickson LC, Gene amplification affecting O(6)-alkylguanine-DNA alkyltransferase activity is not detected in nitrosourea resistant or sensitive human cell lines. Carcinogenesis11:479-83 1990
PubMed ID: 2311191
 
Kunisada T, Miller CD, Schneider EL, Ultraviolet light-induced DNA damage in transcribed sequences: no change in repair with age. Mutat Res237:75-81 1990
PubMed ID: 2164149
 
Mukherjee AB, Wallace KC, Changes in frequency and localization of human X- and Y-chromatin bodies at interphase during in vitro cellular aging. Mech Ageing Dev53(1):61-71 1990
PubMed ID: 2325441
 
Shigeoka H, Yang HC, Early kinase C dependent events in aging human diploid fibroblasts. Mech Ageing Dev55:49-59 1990
PubMed ID: 1698234
 
Sharma PM, Reddy GR, Babior BM, McLachlan A, Alternative splicing of the transcript encoding the human muscle isoenzyme of phosphofructokinase. J Biol Chem265:9006-10 1990
PubMed ID: 2140567
 
Pereira-Smith OM, Stein GH, Robetorye S, Meyer-Demarest S, Immortal phenotype of the HeLa variant D98 is recessive in hybrids formed with normal human fibroblasts. J Cell Physiol143:222-5 1990
PubMed ID: 2332448
 
Bannai S, Sato H, Ishii T, Sugita Y, Induction of cystine transport activity in human fibroblasts by oxygen. J Biol Chem264:18480-4 1989
PubMed ID: 2808385
 
McNearney T, Ballard L, Seya T, Atkinson JP, Membrane cofactor protein of complement is present on human fibroblast, epithelial, and endothelial cells. J Clin Invest84:538-45 1989
PubMed ID: 2474570
 
Matsumura T, Malik F, Holliday R, Levels of DNA methylation in diploid and SV40 transformed human fibroblasts. Exp Gerontol24:477-81 1989
PubMed ID: 2561103
 
Gibson JM, Milam SB, Klebe RJ, Late passage cells display an increase in contractile behavior. Mech Ageing Dev48:101-10 1989
PubMed ID: 2739467
 
Ethier MF, Hickler RB, Dobson JG Jr, Aging increases adenosine and inosine release by human fibroblast cultures. Mech Ageing Dev50:159-68 1989
PubMed ID: 2601414
 
Babich MA, Day RS 3d, Synergistic killing of virus-transformed human cells with interferon and N-methyl-N'-nitro-N-nitrosoguanidine. Carcinogenesis10:265-8 1989
PubMed ID: 2463881
 
Liu AY, Bae-Lee MS, Choi HS, Li BS, Heat shock induction of HSP 89 is regulated in cellular aging. Biochem Biophys Res Commun162:1302-10 1989
PubMed ID: 2764934
 
Liu AY, Lin Z, Choi HS, Sorhage F, Li B, Attenuated induction of heat shock gene expression in aging diploid fibroblasts. J Biol Chem264:12037-45 1989
PubMed ID: 2745427
 
Absher M, Woodcock-Mitchell J, Mitchell J, Baldor L, Low R, Warshaw D, Characterization of vascular smooth muscle cell phenotype in long-term culture. In Vitro Cell Dev Biol25:183-92 1989
PubMed ID: 2921236
 
Quon DV, Proia RL, Fowler AV, Bleibaum J, Neufeld EF, Proteolytic processing of the beta-subunit of the lysosomal enzyme, beta-hexosaminidase, in normal human fibroblasts. J Biol Chem264:3380-4 1989
PubMed ID: 2521634
 
Paw BH, Kaback MM, Neufeld EF, Molecular basis of adult-onset and chronic GM2 gangliosidoses in patients of Ashkenazi Jewish origin: substitution of serine for glycine at position 269 of the alpha-subunit of beta-hexosaminidase [published erratum appears in Proc Natl Acad Sci U S A 1989 Jul;86(14):5625] Proc Natl Acad Sci U S A86:2413-7 1989
PubMed ID: 2522660
 
Boothman DA, Bouvard I, Hughes EN, Identification and characterization of X-ray-induced proteins in human cells. Cancer Res49:2871-8 1989
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Misra BC, Srivatsan ES, Localization of HeLa cell tumor-suppressor gene to the long arm of chromosome II. Am J Hum Genet45:565-77 1989
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Chang ZF, Chen KY, Regulation of ornithine decarboxylase and other cell cycle-dependent genes during senescence of IMR-90 human diploid fibroblasts. J Biol Chem263:11431-5 1988
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Santos MJ, Imanaka T, Shio H, Lazarow PB, Peroxisomal integral membrane proteins in control and Zellweger fibroblasts. J Biol Chem263:10502-9 1988
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Myerowitz R, Splice junction mutation in some Ashkenazi Jews with Tay-Sachs disease: evidence against a single defect within this ethnic group. Proc Natl Acad Sci U S A85:3955-9 1988
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Myerowitz R, Costigan FC, The major defect in Ashkenazi Jews with Tay-Sachs disease is an insertion in the gene for the alpha-chain of beta-hexosaminidase. J Biol Chem263:18587-9 1988
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Dresler SL, Frattini MG, Analysis of butylphenyl-guanine, butylphenyl-deoxyguanosine, and butylphenyl-deoxyguanosine triphosphate inhibition of DNA replication and ultraviolet-induced DNA repair synthesis using permeable human fibroblasts. Biochem Pharmacol37:1033-7 1988
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Paw BH, Neufeld EF, Normal transcription of the beta-hexosaminidase alpha-chain gene in the Ashkenazi Tay-Sachs mutation. J Biol Chem263(6):3012-5 1988
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Slot LA, Hendil KB, Alpha 2-macroglobulin used to isolate intracellular endopeptidases from mammalian cells in culture. Biochem J255(2):437-43 1988
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Noda A, Replicon initiation in normal human cells and in ataxia telangiectasia cells: its differential inhibition by cycloheximide and bleomycin. Cell Biol Int Rep12:943-50 1988
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Wilhelm SM, Collier IE, Kronberger A, Eisen AZ, Marmer BL, Grant GA, Bauer EA, Goldberg GI, Human skin fibroblast stromelysin: structure, glycosylation, substrate specificity, and differential expression in normal and tumorigenic cells. Proc Natl Acad Sci U S A84:6725-9 1987
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Sottile J, Hoyle M, Millis AJ, Enhanced synthesis of a Mr = 55,000 dalton peptide by senescent human fibroblasts. J Cell Physiol131:210-7 1987
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Chen KY, Chang ZF, A marked increase of fucosylation of glycoproteins in IMR-90 human diploid fibroblasts during senescence in vitro. Biochem Biophys Res Commun142:767-74 1987
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Fine A, Goldstein RH, The effect of transforming growth factor-beta on cell proliferation and collagen formation by lung fibroblasts. J Biol Chem262:3897-902 1987
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Constantopoulos G, McGarrity GJ, Activities of oxidative enzymes in mycoplasmas. J Bacteriol169:2012-6 1987
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Cowles EA, Brauker JH, Anderson RL, Turnover of sulfated glycosaminoglycans in fibroblasts derived from patients with Werner's syndrome. Exp Cell Res168:347-57 1987
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Hara H, Kaji H, Random integration of SV40 in SV40-transformed, immortalized human fibroblasts. Exp Cell Res168:531-8 1987
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Dresler SL, Robinson-Hill RM, Direct inhibition of u.v.-induced DNA excision repair in human cells by novobiocin, coumermycin and nalidixic acid. Carcinogenesis8:813-7 1987
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Pettenati MJ, Le Beau MM, Lemons RS, Shima EA, Kawasaki ES, Larson RA, Sherr CJ, Diaz MO, Rowley JD, Assignment of CSF-1 to 5q33.1: evidence for clustering of genes regulating hematopoiesis and for their involvement in the deletion of the long arm of chromosome 5 in myeloid disorders. Proc Natl Acad Sci U S A84:2970-4 1987
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Mann PL, Lopez-Colberg I, Kelley RO, Cell surface oligosaccharide modulation during differentiation. I. Modulation of lectin binding. Mech Ageing Dev38:207-17 1987
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Mann PL, Swartz CM, Kelley RO, Cell surface oligosaccharide modulation during differentiation. II. Membrane mobility of oligosaccharide lectin conjugates. Mech Ageing Dev38:219-30 1987
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Mayer PJ, Bradley MO, Nichols WW, The effect of mild hypothermia (34 degrees C) and mild hyperthermia (39 degrees C) on DNA damage, repair and aging of human diploid fibroblasts. Mech Ageing Dev39:203-22 1987
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Nakamura KD, Hart RW, Comparison of proto oncogene expression in seven primate fibroblast cultures. Mech Ageing Dev39:177-87 1987
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Zokaeem G, Bayleran J, Kaplan P, Hechtman P, Neufeld EF, A shortened beta-hexosaminidase alpha-chain in an Italian patient with infantile Tay-Sachs disease. Am J Hum Genet40:537-47 1987
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Chen KY, Chang ZF, Liu AY, Changes of serum-induced ornithine decarboxylase activity and putrescine content during aging of IMR-90 human diploid fibroblasts. J Cell Physiol129:142-6 1986
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Sanford KK, Parshad R, Stanbridge EJ, Frost JK, Jones GM, Wilkinson JE, Tarone RE, Chromosomal radiosensitivity during the G2 cell cycle period and cytopathology of human normal x tumor cell hybrids. Cancer Res46:2045-9 1986
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Mayer PJ, Bradley MO, Nichols WW, No change in DNA damage or repair of single- and double-strand breaks as human diploid fibroblasts age in vitro. Exp Cell Res166:497-509 1986
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Krusius T, Ruoslahti E, Primary structure of an extracellular matrix proteoglycan core protein deduced from cloned cDNA. Proc Natl Acad Sci U S A83:7683-7 1986
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Myerowitz R, Hogikyan ND, Different mutations in Ashkenazi Jewish and non-Jewish French Canadians with Tay-Sachs disease. Science232:1646-8 1986
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Bunn CL, Baron JI, Mitchell D, Inherent resistance of HeLa cell derivatives to paromomycin. In Vitro Cell Dev Biol22:381-6 1986
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Goldstein RH, Sakowski S, Meeker D, Franzblau C, Polgar P, The effect of prostaglandin E2 (PGE2) on amino acid uptake and protein formation by lung fibroblasts. J Biol Chem261:8734-7 1986
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Delgado D, Raymond L, Dean R, C-ras expression decreases during in vitro senescence in human fibroblasts. Biochem Biophys Res Commun137:917-21 1986
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Dice JF, Chiang HL, Spencer EP, Backer JM, Regulation of catabolism of microinjected ribonuclease A. Identification of residues 7-11 as the essential pentapeptide [published erratum appears in J Biol Chem 1986 Oct 5;261(28):13387] J Biol Chem261:6853-9 1986
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Kulesh DA, Greene JJ, Shape-dependent regulation of proliferation in normal and malignant human cells and its alteration by interferon. Cancer Res46:2793-7 1986
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Liu AY, Chang ZF, Chen KY, Increased level of cAMP-dependent protein kinase in aging human lung fibroblasts. J Cell Physiol128:149-54 1986
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Ramanathan B, Smerdon MJ, Changes in nuclear protein acetylation in u.v.-damaged human cells. Carcinogenesis7:1087-94 1986
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Chen KY, Chang ZF, Age dependency of the metabolic conversion of polyamines into amino acids in IMR-90 human embryonic lung diploid fibroblasts. J Cell Physiol128:27-32 1986
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Ohno T, Kako R, Sato C, Ohkawa A, Distinction of G0 cells from senescent cells in cultures of non-cycling human fetal lung fibroblasts by anti-MAP-1 monoclonal antibody staining. Exp Cell Res163:309-16 1986
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Gibson NW, Hartley J, La France RJ, Vaughan K, Differential cytotoxicity and DNA-damaging effects produced in human cells of the Mer+ and Mer- phenotypes by a series of alkyltriazenylimidazoles. Carcinogenesis7:259-65 1986
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Krauss SW, Linn S, Studies of DNA polymerases alpha and beta from cultured human cells in various replicative states. J Cell Physiol126:99-106 1986
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Gibson NW, Hartley JA, Kohn KW, DNA reactivity and in vitro cytotoxicity of the novel antitumor agent 1,5,2,4-dioxadithiepane-2,2,4,4-tetraoxide (NSC-348948) in human embryo cells. Cancer Res46:1679-83 1986
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Dean R, Kim SS, Delgado D, Expression of c-myc oncogene in human fibroblasts during in vitro senescence. Biochem Biophys Res Commun135:105-9 1986
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Bannai S, Exchange of cystine and glutamate across plasma membrane of human fibroblasts. J Biol Chem261:2256-63 1986
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Pothoulakis C, Barone LM, Ely R, Faris B, Clark ME, Franzblau C, LaMont JT, Purification and properties of Clostridium difficile cytotoxin B. J Biol Chem261:1316-21 1986
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Stein GH, Atkins L, Beeson M, Gordon L, Quiescent human diploid fibroblasts. Common mechanism for inhibition of DNA replication in density-inhibited and serum-deprived cells. Exp Cell Res162:255-60 1986
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Yen PH, Mohandas T, Shapiro LJ, Stability of DNA methylation of the human hypoxanthine phosphoribosyltransferase gene. Somat Cell Mol Genet12:153-61 1986
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Smerdon MJ, Completion of excision repair in human cells. Relationship between ligation and nucleosome formation. J Biol Chem261:244-52 1986
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Henner WD, Blazka ME, Hypersensitivity of cultured ataxia-telangiectasia cells to etoposide. J Natl Cancer Inst76(6):1007-11 1986
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Slot LA, Lauridsen AM, Hendil KB, Intracellular protein degradation in serum-deprived human fibroblasts. Biochem J237(2):491-8 1986
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Hiramatsu K, Masuda M, Yoshikura H, Mode of transmission of human T-cell leukemia virus type I (HTLV I) in a human promyelocytic leukemia HL60 cell. Int J Cancer37(4):601-6 1986
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Hiramatsu K, Yoshikura H, Frequent partial deletion of human adult T-cell leukemia virus type I proviruses in experimental transmission: pattern and possible implication. J Virol58(2):508-12 1986
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Birckbichler PJ, Upchurch HF, Patterson MK Jr, Conway E, A monoclonal antibody to cellular transglutaminase. Hybridoma4(2):179-86 1985
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Santos MJ, Ojeda JM, Garrido J, Leighton F, Peroxisomal organization in normal and cerebrohepatorenal (Zellweger) syndrome fibroblasts. Proc Natl Acad Sci U S A82:6556-60 1985
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Takahashi S, Nakagawa S, Zeydel M, Bhargava G, Ammonia production by IMR-90 fibroblast cultures: effects of ammonia on glutathione, gamma-glutamyl transpeptidase, lysosomal enzymes, and cell division. J Cell Physiol125:107-14 1985
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Stein GH, SV40-transformed human fibroblasts: evidence for cellular aging in pre- crisis cells. J Cell Physiol125:36-44 1985
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Netland PA, Dice JF, Red blood cell-mediated microinjection: methodological considerations. Anal Biochem150:214-20 1985
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Kondo H, Kasuga H, Noumura T, The heterogeneity of human fibroblasts as determined from the effects of hydrocortisone on cell growth and specific dexamethasone binding. Exp Cell Res158:342-8 1985
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Ohno T, Ohkawa A, Sato C, Factors controlling the appearance of the immunofluorescent nuclear dots revealed with monoclonal antibody against microtubule-associated protein-1. Exp Cell Res158:558-62 1985
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McElligott MA, Miao P, Dice JF, Lysosomal degradation of ribonuclease A and ribonuclease S-protein microinjected into the cytosol of human fibroblasts. J Biol Chem260:11986-93 1985
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Kelley RO, Mann PL, Perdue BD, Marek LF, Reduction of filamin in late passage human diploid fibroblasts (IMR-90). Mech Ageing Dev30:79-98 1985
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White FA, Bunn CL, Restriction enzyme analysis of mitochondrial DNA in aging human cells. Mech Ageing Dev30:153-68 1985
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Finlay CA, Rosner BA, Miller JE, Wallace JM, Cristofalo VJ, Enhanced proliferation of WI38 cells in the presence of glucocorticoid- conditioned medium. Exp Cell Res156:462-70 1985
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Hasegawa N, Hanaoka F, Yamada M, Does capacity of DNA replication change during in vitro ageing? Exp Cell Res156:478-86 1985
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Van Riper DA, Absher MP, Lenox RH, Muscarinic receptors on intact human fibroblasts. Absence of receptor activity in adult skin cells. J Clin Invest76:882-6 1985
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Lan SY, Smerdon MJ, A nonuniform distribution of excision repair synthesis in nucleosome core DNA. Biochemistry24:7771-83 1985
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Dresler SL, Stimulation of deoxyribonucleic acid excision repair in human fibroblasts pretreated with sodium butyrate. Biochemistry24:6861-9 1985
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Watkins JF, Smerdon MJ, Nucleosome rearrangement in vitro. 1. Two phases of salt-induced nucleosome migration in nuclei. Biochemistry24:7279-87 1985
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Watkins JF, Smerdon MJ, Nucleosome rearrangement in vitro. 2. Formation of nucleosomes in newly repaired regions of DNA. Biochemistry24:7288-95 1985
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Tomkins DJ, Sisken JE, Abnormalities in the cell-division cycle in Roberts syndrome fibroblasts: a cellular basis for the phenotypic characteristics? Am J Hum Genet36:1332-40 1984
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Bienkowski RS, Collagen degradation in human lung fibroblasts: extent of degradation, role of lysosomal proteases, and evaluation of an alternate hypothesis. J Cell Physiol121:152-8 1984
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Torrelio BM, Paz MA, Gallop PM, Cellular proliferation and hypusine synthesis. Exp Cell Res154:454-63 1984
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Ohno T, Yamada M, Growth stimulation by direct cell-cell contact (contact stimulation) in normal human lung fibroblasts. Biomed Pharmacother38:337-43 1984
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Ohno T, Nishimura T, Nakano K, Kaneko I, Differential recovery from potentially lethal damage in normal human lung fibroblasts after irradiation with 60Co gamma-rays and accelerated N-ion beam. Int J Radiat Biol Relat Stud Phys Chem Med45:21-6 1984
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Ohno T, Yamaguchi N, Life span elongation of Werner's syndrome fibroblasts by co-culture with origin-defective SV-40 DNA transformed cells. Hum Genet68:209-10 1984
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Okada AA, Dice JF, Altered degradation of intracellular proteins in aging human fibroblasts. Mech Ageing Dev26:341-56 1984
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Myerowitz R, Proia RL, cDNA clone for the alpha-chain of human beta-hexosaminidase: deficiency of alpha-chain mRNA in Ashkenazi Tay-Sachs fibroblasts. Proc Natl Acad Sci U S A81:5394-8 1984
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Asselineau D, Bell E, Prolylhydroxylase activation in IMR 90 fibroblasts: state of differentiation rather than population doubling level determines requirements for enzyme activation. Mech Ageing Dev25:257-66 1984
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Straus DS, Pang KJ, Effects of bradykinin on DNA synthesis in resting NIL8 hamster cells and human fibroblasts. Exp Cell Res151:87-95 1984
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Smith CA, Okumoto DS, Nature of DNA repair synthesis resistant to inhibitors of polymerase alpha in human cells. Biochemistry23:1383-91 1984
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Hasegawa N, Hanaoka F, Yamada M, Increased unscheduled DNA synthesis in aged human diploid fibroblasts. Mech Ageing Dev25:297-305 1984
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Goldstein RH, Wall M, Activation of protein formation and cell division by bradykinin and des- Arg9-bradykinin. J Biol Chem259:9263-8 1984
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Absher M, Cristofalo VJ, Analysis of cell division by time-lapse cinematographic studies of hydrocortisone-treated embryonic lung fibroblasts. J Cell Physiol119:315-9 1984
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Sariban E, Erickson LC, Kohn KW, Effects of carbamoylation on cell survival and DNA repair in normal human embryo cells (IMR-90) treated with various 1-(2-chloroethyl)-1- nitrosoureas. Cancer Res44:1352-7 1984
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Yoshikura H, Nishida J, Yoshida M, Kitamura Y, Takaku F, Ikeda S, Isolation of HTLV derived from Japanese adult T-cell leukemia patients in human diploid fibroblast strain IMR90 and the biological characters of the infected cells. Int J Cancer33(6):745-9 1984
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Balin AK, Fisher AJ, Carter DM, Oxygen modulates growth of human cells at physiologic partial pressures. J Exp Med160(1):152-66 1984
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Proia RL, d'Azzo A, Neufeld EF, Association of alpha- and beta-subunits during the biosynthesis of beta- hexosaminidase in cultured human fibroblasts. J Biol Chem259:3350-4 1984
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Stein GH, Human diploid fibroblasts (HDF) can induce DNA synthesis in cycling HDF but not in quiescent HDF or senescent HDF. Exp Cell Res144:468-71 1983
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Weissman BE, Stanbridge EJ, Complexity of control of tumorigenic expression in intraspecies hybrids of human SV40-transformed fibroblasts and normal human fibroblast cell lines. Cytogenet Cell Genet35:263-8 1983
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Wedel N, Toselli P, Pothoulakis C, Faris B, Oliver P, Franzblau C, LaMont T, Ultrastructural effects of Clostridium difficile toxin B on smooth muscle cells and fibroblasts. Exp Cell Res148:413-22 1983
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Roff CF, Wozniak RW, Blenis J, Wang JL, The effect of mannose6-phosphate on the turnover of cell surface glycosaminoglycans. Exp Cell Res144:333-44 1983
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Hildebran JN, Absher M, Low RB, Altered rates of collagen synthesis in in vitro aged human lung fibroblasts. In Vitro19:307-14 1983
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Bemiller P, Kraus ER Jr, The effect of clinical and subclinical concentrations of lead on the in vitro aging of human fibroblasts. Mech Ageing Dev22:79-87 1983
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Halton DM, Peterson WD Jr, Hukku B, Cell culture quality control by rapid isoenzymatic characterization. In Vitro19:16-24 1983
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Chung HR, Albanese EA, Studzinski GP, Differential effect of coformycin on the cell cycle traverse of normal and simian virus 40-transformed human fibroblasts. Cancer Res43:1269-74 1983
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Wilson VL, Jones PA, DNA methylation decreases in aging but not in immortal cells. Science220:1055-7 1983
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nn JM, Bodner JB, Knowles SE, Ballard FJ, Inhibition of protein breakdown by epidermal growth factor in IMR90 human fibroblasts and other mammalian cell lines Biochem J210(1):251-8 1983
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Backer JM, Bourret L, Dice JF, Regulation of catabolism of microinjected ribonuclease A requires the amino-terminal 20 amino acids. Proc Natl Acad Sci U S A80:2166-70 1983
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Absher M, Sylwester D, Hart BA, Time-lapse cinematographic analysis of beryllium--lung fibroblast interactions. Environ Res30:34-43 1983
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Becker D, Lane MA, Cooper GM, Identification of an antigen associated with transforming genes of human and mouse mammary carcinomas. Proc Natl Acad Sci U S A79:3315-9 1982
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Duthu GS, Braunschweiger KI, Pereira-Smith OM, Norwood TH, Smith JR, A long-lived human diploid fibroblast line for cellular aging studies: applications in cell hybridization. Mech Ageing Dev20:243-52 1982
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Anderson PJ, Actin and tubulin in normal and cystic fibrosis fibroblasts. Biochem Biophys Res Commun108:182-5 1982
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Ware JL, Paulson DF, Parks SF, Webb KS, Production of monoclonal antibody alpha Pro3 recognizing a human prostatic carcinoma antigen. Cancer Res42:1215-22 1982
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Bannai S, Ishii T, Transport of cystine and cysteine and cell growth in cultured human diploid fibroblasts: effect of glutamate and homocysteate. J Cell Physiol112:265-72 1982
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Lubin M, Cahn F, Coutermarsh BA, Amiloride, protein synthesis, and activation of quiescent cells. J Cell Physiol113:247-51 1982
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Torrelio BM, Paz MA, Gallop PM, Modulation of purine synthesis and phosphoribosylpyrophosphate content in human fibroblasts at different population doublings. Mech Ageing Dev19:147-58 1982
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Starling JJ, Sieg SM, Beckett ML, Schellhammer PF, Ladaga LE, Wright GL Jr, Monoclonal antibodies to human prostate and bladder tumor-associated antigens. Cancer Res42:3084-9 1982
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Smerdon MJ, Watkins JF, Lieberman MW, Effect of histone H1 removal on the distribution of ultraviolet-induced deoxyribonucleic acid repair synthesis within chromatin. Biochemistry21:3879-85 1982
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Sterling KM Jr, DiPetrillo TA, Kotch JP, Cutroneo KR, Bleomycin-induced increase of collagen turnover in IMR-90 fibroblasts: an in vitro model of connective tissue restructuring during lung fibrosis. Cancer Res42:3502-6 1982
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Stanbridge EJ, Der CJ, Doersen CJ, Nishimi RY, Peehl DM, Weissman BE, Wilkinson JE, Human cell hybrids: analysis of transformation and tumorigenicity. Science215:252-9 1982
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Carnicero HH, Englard S, Seifter S, Carnitine uptake and fatty acid utilization by diploid cells aging in culture. Arch Biochem Biophys215:78-88 1982
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Hasegawa N, Hanaoka F, Hori T, Yamada M, Reevaluation of DNA chain elongation rate in human diploid fibroblasts. Exp Cell Res140:443-7 1982
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Stanbridge EJ, Rosen SW, Sussman HH, Expression of the alpha subunit of human chorionic gonadotropin is specifically correlated with tumorigenic expression in human cell hybrids. Proc Natl Acad Sci U S A79:6242-5 1982
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Ballard FJ, Nield MK, Francis GL, Dahlenburg GW, Wallace JC, The relationship between the insulin content and inhibitory effects of bovine colostrum on protein breakdown in cultured cells. J Cell Physiol110(3):249-54 1982
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Lamon KD, Chiger JL, Kaji H, Effects of hydrocortisone and aspirin on protein synthesis and post-translational protein modification in cultured cells Biochem Pharmacol31(11):2047-52 1982
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Wilson GN, Szura LL, Rushford C, Jackson D, Erickson J, Structure and variation of human ribosomal DNA: the external transcribed spacer and adjacent regions. Am J Hum Genet34:32-49 1982
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Fong D, Poole B, The effect of canavanine on protein synthesis and protein degradation in IMR-90 fibroblasts. Biochim Biophys Acta696:193-200 1982
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Gallop PM, Paz MA, Henson E, Boradeption: a new procedure for transferring water-insoluble agents across cell membranes. Science217:166-9 1982
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Namba M, Fukushima F, Kimoto T, Effects of feeder layers made of human, mouse, hamster, and rat cells on the cloning efficiency of transformed human cells. In Vitro18:469-75 1982
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Vogel KG, Peterson DW, Extracellular, surface, and intracellular proteoglycans produced by human embryo lung fibroblasts in culture (IMR-90). J Biol Chem256:13235-42 1981
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Kelley J, Trombley L, Kovacs EJ, Davis GS, Absher M, Pulmonary macrophages alter the collagen phenotype of lung fibroblasts. J Cell Physiol109:353-61 1981
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Didinsky JB, Rheinwald JG, Failure of hydrocortisone or growth factors to influence the senescence of fibroblasts in a new culture system for assessing replicative lifespan. J Cell Physiol109:171-9 1981
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Wu RS, Bonner WM, Separation of basal histone synthesis from S-phase histone synthesis in dividing cells. Cell27:321-30 1981
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Imai K, Galloway DR, Ferrone S, Serological and immunochemical analysis of the specificity of xenoantiserum 8986 elicited with hybrids between human melanoma cells and murine fibroblasts. Cancer Res41:1028-33 1981
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Der CJ, Stanbridge EJ, A tumor-specific membrane phosphoprotein marker in human cell hybrids. Cell26:429-38 1981
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Paz MA, Torrelio M, Gallop PM, X-linked processes in serially passaged "aging" human diploid cells. J Gerontol36:142-51 1981
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Vogel KG, Kendall VF, Sapien RE, Glycosaminoglycan synthesis and composition in human fibroblasts during in vitro cellular aging (IMR-90). J Cell Physiol107:271-81 1981
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Bradley MO, Taylor VI, DNA double-strand breaks induced in normal human cells during the repair of ultraviolet light damage. Proc Natl Acad Sci U S A78:3619-23 1981
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Morgan AC Jr, Galloway DR, Jensen FC, Giovanella BC, Reisfeld RA, Immunochemical delineation of an oncofetal antigen on normal and simian virus 40-transformed human fetal melanocytes. Proc Natl Acad Sci U S A78:3834-8 1981
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Stein GH, Yanishevsky RM, Quiescent human diploid cells can inhibit entry into S phase in replicative nuclei in heterodikaryons. Proc Natl Acad Sci U S A78:3025-9 1981
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Nohga K, Reddy R, Busch H, Comparison of RNase T1 fingerprints of U1, U2, and U3 small nuclear RNA's of HeLa cells, human normal fibroblasts, and Novikoff hepatoma cells. Cancer Res41:2215-20 1981
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Fox N, Fernandez C, Studzinski GP, Visualization of nucleolar substructure in cultured human fibroblasts by magnesium-activated adenosine triphosphatase reaction. J Histochem Cytochem29:1115-20 1981
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Carnicero HH, Adamany AM, Englard S, Collagen glucosyl- and galactosyltransferases of cultured human fetal lung fibroblasts. Arch Biochem Biophys210:678-90 1981
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McCurry LS, Jacobson MK, Poly(ADP-ribose) synthesis following DNA damage in cells heterozygous or homozygous for the xeroderma pigmentosum genotype. J Biol Chem256:551-3 1981
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Wu BC, Spohn WH, Busch H, Comparison of nuclear proteins of several human tumors and normal cells by two-dimensional gel electrophoresis. Cancer Res41:336-42 1981
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Hara H, Lamon KD, Kaji H, Effect of temperature on normal and SV40-transformed human fibroblasts. Biochim Biophys Acta673(1):37-45 1981
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Lane MA, Sainten A, Cooper GM, Activation of related transforming genes in mouse and human mammary carcinomas. Proc Natl Acad Sci U S A78:5185-9 1981
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Wright WE, Recovery of heterokaryons at high cell densities following isolation using irreversible biochemical inhibitors. Somatic Cell Genet7:769-75 1981
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Neff NT, Bourret L, Miao P, Dice JF, Degradation of proteins microinjected into IMR-90 human diploid fibroblasts. J Cell Biol91:184-94 1981
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Ochs DC, McConkey EH, Guard NL, Vimentin-derived proteins: differences between normal human fibroblasts and transformed human cells. Exp Cell Res135:355-62 1981
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Bourgeois CA, Raynaud N, Diatloff-Zito C, Macieira-Coehlo A, Effect of low dose rate ionizing radiation on the division potential of cells in vitro. VIII. Cytogenetic analysis of human fibroblasts. Mech Ageing Dev17:225-35 1981
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Taylor L, Schneider E, Smith J, Polgar P, Prostaglandin production and cellular aging. Mech Ageing Dev16:311-7 1981
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Vogel KG, Kelley RO, Stewart C, Loss of organized fibronectin matrix from the surface of aging diploid fibroblasts (IMR-90). Mech Ageing Dev16:295-302 1981
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View karyotype 
karyotype 
PDL at Senescence 62
Cumulative PDL at Freeze 19
Passage Frozen 5
Split Ratio 1:5
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids
Serum 15% fetal bovine serum Not inactivated
Substrate None specified
Subcultivation Method trypsin-EDTA