Repository NIA Aging Cell Culture Repository
Subcollection IMR90/IMR91 Cell Cultures
Specially Characterized Fibroblasts
Biopsy Source Lung
Cell Type Fibroblast
Tissue Type Lung
Transformant Untransformed
Race Caucasian
Relation to Proband proband
Confirmation Clinical summary/Case history
Species Homo sapiens
Common Name Human
Remarks The IMR-90 line was established on 7/7/75 using explants of minced lung tissue obtained from a clinically normal 16 week fetus. The karyotype is 46,XX; normal diploid female. No variant isoenzyme patterns found for lactate dehydrogenase, malate dehydrogenase, 6-phosphogluconate dehydrogenase, and glucose-6-phosphate dehydrogenase; G6PD is isoenzyme type B. The HLA type is A2, B12, BW40, B5. Reverse transcriptase was not detected. The maximum number of population doublings achieved to senescence range from 58-73. Earlier passage IMR-90 cells available only with permission of NIA: see guidelines in Science 196:60-63 (1977).
PDL at Senescence 62
PDL at Freeze 19
Passage Frozen 5
Remark The IMR-90 line was established on 7/7/75 using explants of minced lung tissue obtained from a clinically normal 16 week fetus. The karyotype is 46,XX; normal diploid female. No variant isoenzyme patterns found for lactate dehydrogenase, malate dehydrogenase, 6-phosphogluconate dehydrogenase, and glucose-6-phosphate dehydrogenase; G6PD is isoenzyme type B. The HLA type is A2, B12, BW40, B5. Reverse transcriptase was not detected. The maximum number of population doublings achieved to senescence range from 58-73. Earlier passage IMR-90 cells available only with permission of NIA: see guidelines in Science 196:60-63 (1977).
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Forsyth NR, Evans AP, Shay JW, Wright WE, Developmental differences in the immortalization of lung fibroblasts by telomerase. Aging Cell2(5):235-43 2003
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Li GZ, Eller MS, Firoozabadi R, Gilchrest BA, Evidence that exposure of the telomere 3' overhang sequence induces senescence Proceedings of the National Academy of Sciences of the United States of America100:527-31 2003
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Thannickal VJ, Lee DY, White ES, Cui Z, Larios JM, Chacon R, Horowitz JC, Day RM, Thomas PE, Myofibroblast differentiation by transforming growth factor-beta1 is dependent on cell adhesion and integrin signaling via focal adhesion kinase The Journal of biological chemistry278:12384-9 2003
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Parada C, Hernandez Losa J, Guinea J, Sanchez-Arevalo V, Fernandez Soria V, Alvarez-Vallina L, Sanchez-Prieto R, Ramon Y Cajal S, Adenovirus E1a protein enhances the cytotoxic effects of the herpes thymidine kinase-ganciclovir system. Cancer Gene Ther10(2):152-60 2003
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Baird DM, Rowson J, Wynford-Thomas D, Kipling D, Extensive allelic variation and ultrashort telomeres in senescent human cells. Nat Genet33(2):203-7 2003
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Deddish PA, Marcic BM, Tan F, Jackman HL, Chen Z, Erdos EG, Neprilysin inhibitors potentiate effects of bradykinin on b2 receptor. Hypertension39(2 Pt 2):619-23 2002
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Zhang X, Shu L, Hosoi H, Murti KG, Houghton PJ, Predominant nuclear localization of mammalian target of rapamycin in normal and malignant cells in culture. J Biol Chem277(31):28127-34 2002
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Shreeram S, Sparks A, Lane DP, Blow JJ, Cell type-specific responses of human cells to inhibition of replication licensing. Oncogene21(43):6624-32 2002
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Yazdi PT, Wang Y, Zhao S, Patel N, Lee EY, Qin J, SMC1 is a downstream effector in the ATM/NBS1 branch of the human S-phase checkpoint. Genes Dev16(5):571-82 2002
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Tang D, Wu D, Hirao A, Lahti JM, Liu L, Mazza B, Kidd VJ, Mak TW, Ingram AJ, ERK activation mediates cell cycle arrest and apoptosis after DNA damage independently of p53. J Biol Chemepub ahead of print:9819-24 2002
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Danam RP, Howell SR, Remack JS, Brent TP, Heterogeneous methylation of the O(6)-methylguanine-DNA methyltransferase promoter in immortalized IMR90 cell lines. Int J Oncol18(6):1187-93 2001
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Yang X, Taylor L, Polgar P, p53 down-regulates human bradykinin B1 receptor gene expression. J Cell Biochem82(1):38-45 2001
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Yang X, Taylor L, Yu J, Fenton MJ, Polgar P, Mediator caused induction of a human bradykinin B1 receptor minigene: participation of c-Jun in the process. J Cell Biochem82(1):163-70 2001
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Guo W, Okamoto M, Park NH, Lee YM, Park NH, Cloning and expression of hamster telomerase catalytic subunit cDNA. Int J Mol Med8(1):73-8 2001
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Atamna H, Liu J, Ames BN, Heme deficiency selectively interrupts assembly of mitochondrial complex IV in human fibroblasts: revelance to aging. J Biol Chem276(51):48410-6 2001
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Atamna H, Robinson C, Ingersoll R, Elliott H, Ames BN, N-t-Butyl hydroxylamine is an antioxidant that reverses age-related changes in mitochondria in vivo and in vitro. FASEB J15(12):2196-204 2001
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Atamna H, Cheung I, Ames BN, A method for detecting abasic sites in living cells: age-dependent changes in base excision repair. Proc Natl Acad Sci U S A97(2):686-91 2000
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Yang X, He Z, Xin B, Cao L, LMP1 of Epstein-Barr virus suppresses cellular senescence associated with the inhibition of p16INK4a expression Oncogene19(16):2002-13 2000
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Ferbeyre G, de Stanchina E, Querido E, Baptiste N, Prives C, Lowe SW, PML is induced by oncogenic ras and promotes premature senescence. Genes Dev14(16):2015-27 2000
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Atamna H, Paler-Martinez A, Ames BN, N-t-butyl hydroxylamine, a hydrolysis product of alpha-phenyl-N-t-butyl nitrone, is more potent in delaying senescence in human lung fibroblasts. J Biol Chem275:6741-8 2000
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Martelli F, Livingston DM, Regulation of endogenous E2F1 stability by the retinoblastoma family proteins. Proc Natl Acad Sci U S A96:2858-63 1999
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Ouellette MM, Aisner DL, Savre-Train I, Wright WE, Shay JW, Telomerase activity does not always imply telomere maintenance. Biochem Biophys Res Commun254(3):795-803 1999
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Rudolph C, Adam G, Simm A, Determination of copy number of c-Myc protein per cell by quantitative Western blotting. Anal Biochem269(1):66-71 1999
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Chiou WJ, Wang J, Berg CE, Wu-Wong JR, SV40 virus transformation down-regulates endothelin receptor. Biochim Biophys Acta1450(1):35-44 1999
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Suzuki K, Kazui T, Yoshida M, Uno T, Kobayashi T, Kimura T, Yoshida T, Sugimura H, Drug-induced apoptosis and p53, BCL-2 and BAX expression in breast cancer tissues in vivo and in fibroblast cells in vitro. Jpn J Clin Oncol29(7):323-31 1999
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Karlseder J, Broccoli D, Dai Y, Hardy S, de Lange T, p53- and ATM-dependent apoptosis induced by telomeres lacking TRF2. Science283(5406):1321-5 1999
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Yang X, Taylor L, Polgar P, Mechanisms in the transcriptional regulation of bradykinin B1 receptor gene expression. Identification of a minimum cell-type specific enhancer. J Biol Chem273(17):10763-70 1998
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Lukiw WJ, Bazan NG, Strong nuclear factor-kappaB-DNA binding parallels cyclooxygenase-2 gene transcription in aging and in sporadic Alzheimer's disease superior temporal lobe neocortex. J Neurosci Res53(5):583-92 1998
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Choung J, Taylor L, Thomas K, Zhou X, Kagan H, Yang X, Polgar P, Role of EP2 receptors and cAMP in prostaglandin E2 regulated expression of type I collagen alpha1, lysyl oxidase, and cyclooxygenase-1 genes in human embryo lung fibroblasts. J Cell Biochem71(2):254-63 1998
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Jiang WQ, Ringertz N, Altered distribution of the promyelocytic leukemia-associated protein is associated with cellular senescence. Cell Growth Differ8(5):513-22 1997
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Baldin V, Cans C, Watanabe N, Ducommun B, Evidence for a mammalian Nim1-like kinase pathway acting at the G0-1/S transition. Biochem Biophys Res Commun236(1):130-4 1997
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Krupsky M, Kuang PP, Goldstein RH, Regulation of type I collagen mRNA by amino acid deprivation in human lung fibroblasts. J Biol Chem272:13864-8 1997
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Makarov VL, Hirose Y, Langmore JP, Long G tails at both ends of human chromosomes suggest a C strand degradation mechanism for telomere shortening. Cell88:657-66 1997
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Saito H, Papaconstantinou J, Sato H, Goldstein S, Regulation of a novel gene encoding a lysyl oxidase-related protein in cellular adhesion and senescence. J Biol Chem272:8157-60 1997
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Myerowitz R, Splice junction mutation in some Ashkenazi Jews with Tay-Sachs disease: evidence against a single defect within this ethnic group. Proc Natl Acad Sci U S A85:3955-9 1988
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Myerowitz R, Costigan FC, The major defect in Ashkenazi Jews with Tay-Sachs disease is an insertion in the gene for the alpha-chain of beta-hexosaminidase. J Biol Chem263:18587-9 1988
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Dresler SL, Frattini MG, Analysis of butylphenyl-guanine, butylphenyl-deoxyguanosine, and butylphenyl-deoxyguanosine triphosphate inhibition of DNA replication and ultraviolet-induced DNA repair synthesis using permeable human fibroblasts. Biochem Pharmacol37:1033-7 1988
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Wilhelm SM, Collier IE, Kronberger A, Eisen AZ, Marmer BL, Grant GA, Bauer EA, Goldberg GI, Human skin fibroblast stromelysin: structure, glycosylation, substrate specificity, and differential expression in normal and tumorigenic cells. Proc Natl Acad Sci U S A84:6725-9 1987
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Chen KY, Chang ZF, A marked increase of fucosylation of glycoproteins in IMR-90 human diploid fibroblasts during senescence in vitro. Biochem Biophys Res Commun142:767-74 1987
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Dresler SL, Robinson-Hill RM, Direct inhibition of u.v.-induced DNA excision repair in human cells by novobiocin, coumermycin and nalidixic acid. Carcinogenesis8:813-7 1987
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Pettenati MJ, Le Beau MM, Lemons RS, Shima EA, Kawasaki ES, Larson RA, Sherr CJ, Diaz MO, Rowley JD, Assignment of CSF-1 to 5q33.1: evidence for clustering of genes regulating hematopoiesis and for their involvement in the deletion of the long arm of chromosome 5 in myeloid disorders. Proc Natl Acad Sci U S A84:2970-4 1987
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Mann PL, Swartz CM, Kelley RO, Cell surface oligosaccharide modulation during differentiation. II. Membrane mobility of oligosaccharide lectin conjugates. Mech Ageing Dev38:219-30 1987
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Mayer PJ, Bradley MO, Nichols WW, The effect of mild hypothermia (34 degrees C) and mild hyperthermia (39 degrees C) on DNA damage, repair and aging of human diploid fibroblasts. Mech Ageing Dev39:203-22 1987
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Nakamura KD, Hart RW, Comparison of proto oncogene expression in seven primate fibroblast cultures. Mech Ageing Dev39:177-87 1987
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Zokaeem G, Bayleran J, Kaplan P, Hechtman P, Neufeld EF, A shortened beta-hexosaminidase alpha-chain in an Italian patient with infantile Tay-Sachs disease. Am J Hum Genet40:537-47 1987
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Goldstein RH, Sakowski S, Meeker D, Franzblau C, Polgar P, The effect of prostaglandin E2 (PGE2) on amino acid uptake and protein formation by lung fibroblasts. J Biol Chem261:8734-7 1986
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Delgado D, Raymond L, Dean R, C-ras expression decreases during in vitro senescence in human fibroblasts. Biochem Biophys Res Commun137:917-21 1986
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Dice JF, Chiang HL, Spencer EP, Backer JM, Regulation of catabolism of microinjected ribonuclease A. Identification of residues 7-11 as the essential pentapeptide [published erratum appears in J Biol Chem 1986 Oct 5;261(28):13387] J Biol Chem261:6853-9 1986
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Kulesh DA, Greene JJ, Shape-dependent regulation of proliferation in normal and malignant human cells and its alteration by interferon. Cancer Res46:2793-7 1986
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Liu AY, Chang ZF, Chen KY, Increased level of cAMP-dependent protein kinase in aging human lung fibroblasts. J Cell Physiol128:149-54 1986
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Ramanathan B, Smerdon MJ, Changes in nuclear protein acetylation in u.v.-damaged human cells. Carcinogenesis7:1087-94 1986
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Chen KY, Chang ZF, Age dependency of the metabolic conversion of polyamines into amino acids in IMR-90 human embryonic lung diploid fibroblasts. J Cell Physiol128:27-32 1986
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Ohno T, Kako R, Sato C, Ohkawa A, Distinction of G0 cells from senescent cells in cultures of non-cycling human fetal lung fibroblasts by anti-MAP-1 monoclonal antibody staining. Exp Cell Res163:309-16 1986
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Gibson NW, Hartley J, La France RJ, Vaughan K, Differential cytotoxicity and DNA-damaging effects produced in human cells of the Mer+ and Mer- phenotypes by a series of alkyltriazenylimidazoles. Carcinogenesis7:259-65 1986
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Krauss SW, Linn S, Studies of DNA polymerases alpha and beta from cultured human cells in various replicative states. J Cell Physiol126:99-106 1986
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Gibson NW, Hartley JA, Kohn KW, DNA reactivity and in vitro cytotoxicity of the novel antitumor agent 1,5,2,4-dioxadithiepane-2,2,4,4-tetraoxide (NSC-348948) in human embryo cells. Cancer Res46:1679-83 1986
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Dean R, Kim SS, Delgado D, Expression of c-myc oncogene in human fibroblasts during in vitro senescence. Biochem Biophys Res Commun135:105-9 1986
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Bannai S, Exchange of cystine and glutamate across plasma membrane of human fibroblasts. J Biol Chem261:2256-63 1986
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Pothoulakis C, Barone LM, Ely R, Faris B, Clark ME, Franzblau C, LaMont JT, Purification and properties of Clostridium difficile cytotoxin B. J Biol Chem261:1316-21 1986
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Stein GH, Atkins L, Beeson M, Gordon L, Quiescent human diploid fibroblasts. Common mechanism for inhibition of DNA replication in density-inhibited and serum-deprived cells. Exp Cell Res162:255-60 1986
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Yen PH, Mohandas T, Shapiro LJ, Stability of DNA methylation of the human hypoxanthine phosphoribosyltransferase gene. Somat Cell Mol Genet12:153-61 1986
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Myerowitz R, Hogikyan ND, Different mutations in Ashkenazi Jewish and non-Jewish French Canadians with Tay-Sachs disease. Science232:1646-8 1986
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Bunn CL, Baron JI, Mitchell D, Inherent resistance of HeLa cell derivatives to paromomycin. In Vitro Cell Dev Biol22:381-6 1986
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Chen KY, Chang ZF, Liu AY, Changes of serum-induced ornithine decarboxylase activity and putrescine content during aging of IMR-90 human diploid fibroblasts. J Cell Physiol129:142-6 1986
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Sanford KK, Parshad R, Stanbridge EJ, Frost JK, Jones GM, Wilkinson JE, Tarone RE, Chromosomal radiosensitivity during the G2 cell cycle period and cytopathology of human normal x tumor cell hybrids. Cancer Res46:2045-9 1986
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Mayer PJ, Bradley MO, Nichols WW, No change in DNA damage or repair of single- and double-strand breaks as human diploid fibroblasts age in vitro. Exp Cell Res166:497-509 1986
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Krusius T, Ruoslahti E, Primary structure of an extracellular matrix proteoglycan core protein deduced from cloned cDNA. Proc Natl Acad Sci U S A83:7683-7 1986
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Smerdon MJ, Completion of excision repair in human cells. Relationship between ligation and nucleosome formation. J Biol Chem261:244-52 1986
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Hiramatsu K, Masuda M, Yoshikura H, Mode of transmission of human T-cell leukemia virus type I (HTLV I) in a human promyelocytic leukemia HL60 cell. Int J Cancer37(4):601-6 1986
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Hiramatsu K, Yoshikura H, Frequent partial deletion of human adult T-cell leukemia virus type I proviruses in experimental transmission: pattern and possible implication. J Virol58(2):508-12 1986
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Henner WD, Blazka ME, Hypersensitivity of cultured ataxia-telangiectasia cells to etoposide. J Natl Cancer Inst76(6):1007-11 1986
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Slot LA, Lauridsen AM, Hendil KB, Intracellular protein degradation in serum-deprived human fibroblasts. Biochem J237(2):491-8 1986
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Birckbichler PJ, Upchurch HF, Patterson MK Jr, Conway E, A monoclonal antibody to cellular transglutaminase. Hybridoma4(2):179-86 1985
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Santos MJ, Ojeda JM, Garrido J, Leighton F, Peroxisomal organization in normal and cerebrohepatorenal (Zellweger) syndrome fibroblasts. Proc Natl Acad Sci U S A82:6556-60 1985
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Takahashi S, Nakagawa S, Zeydel M, Bhargava G, Ammonia production by IMR-90 fibroblast cultures: effects of ammonia on glutathione, gamma-glutamyl transpeptidase, lysosomal enzymes, and cell division. J Cell Physiol125:107-14 1985
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Stein GH, SV40-transformed human fibroblasts: evidence for cellular aging in pre- crisis cells. J Cell Physiol125:36-44 1985
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Netland PA, Dice JF, Red blood cell-mediated microinjection: methodological considerations. Anal Biochem150:214-20 1985
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Kondo H, Kasuga H, Noumura T, The heterogeneity of human fibroblasts as determined from the effects of hydrocortisone on cell growth and specific dexamethasone binding. Exp Cell Res158:342-8 1985
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Ohno T, Ohkawa A, Sato C, Factors controlling the appearance of the immunofluorescent nuclear dots revealed with monoclonal antibody against microtubule-associated protein-1. Exp Cell Res158:558-62 1985
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McElligott MA, Miao P, Dice JF, Lysosomal degradation of ribonuclease A and ribonuclease S-protein microinjected into the cytosol of human fibroblasts. J Biol Chem260:11986-93 1985
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Kelley RO, Mann PL, Perdue BD, Marek LF, Reduction of filamin in late passage human diploid fibroblasts (IMR-90). Mech Ageing Dev30:79-98 1985
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White FA, Bunn CL, Restriction enzyme analysis of mitochondrial DNA in aging human cells. Mech Ageing Dev30:153-68 1985
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Finlay CA, Rosner BA, Miller JE, Wallace JM, Cristofalo VJ, Enhanced proliferation of WI38 cells in the presence of glucocorticoid- conditioned medium. Exp Cell Res156:462-70 1985
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Hasegawa N, Hanaoka F, Yamada M, Does capacity of DNA replication change during in vitro ageing? Exp Cell Res156:478-86 1985
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Van Riper DA, Absher MP, Lenox RH, Muscarinic receptors on intact human fibroblasts. Absence of receptor activity in adult skin cells. J Clin Invest76:882-6 1985
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Lan SY, Smerdon MJ, A nonuniform distribution of excision repair synthesis in nucleosome core DNA. Biochemistry24:7771-83 1985
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Dresler SL, Stimulation of deoxyribonucleic acid excision repair in human fibroblasts pretreated with sodium butyrate. Biochemistry24:6861-9 1985
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Watkins JF, Smerdon MJ, Nucleosome rearrangement in vitro. 1. Two phases of salt-induced nucleosome migration in nuclei. Biochemistry24:7279-87 1985
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Watkins JF, Smerdon MJ, Nucleosome rearrangement in vitro. 2. Formation of nucleosomes in newly repaired regions of DNA. Biochemistry24:7288-95 1985
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Bienkowski RS, Collagen degradation in human lung fibroblasts: extent of degradation, role of lysosomal proteases, and evaluation of an alternate hypothesis. J Cell Physiol121:152-8 1984
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Torrelio BM, Paz MA, Gallop PM, Cellular proliferation and hypusine synthesis. Exp Cell Res154:454-63 1984
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Okada AA, Dice JF, Altered degradation of intracellular proteins in aging human fibroblasts. Mech Ageing Dev26:341-56 1984
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Myerowitz R, Proia RL, cDNA clone for the alpha-chain of human beta-hexosaminidase: deficiency of alpha-chain mRNA in Ashkenazi Tay-Sachs fibroblasts. Proc Natl Acad Sci U S A81:5394-8 1984
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Ohno T, Yamada M, Growth stimulation by direct cell-cell contact (contact stimulation) in normal human lung fibroblasts. Biomed Pharmacother38:337-43 1984
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Ohno T, Nishimura T, Nakano K, Kaneko I, Differential recovery from potentially lethal damage in normal human lung fibroblasts after irradiation with 60Co gamma-rays and accelerated N-ion beam. Int J Radiat Biol Relat Stud Phys Chem Med45:21-6 1984
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Ohno T, Yamaguchi N, Life span elongation of Werner's syndrome fibroblasts by co-culture with origin-defective SV-40 DNA transformed cells. Hum Genet68:209-10 1984
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Tomkins DJ, Sisken JE, Abnormalities in the cell-division cycle in Roberts syndrome fibroblasts: a cellular basis for the phenotypic characteristics? Am J Hum Genet36:1332-40 1984
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Smith CA, Okumoto DS, Nature of DNA repair synthesis resistant to inhibitors of polymerase alpha in human cells. Biochemistry23:1383-91 1984
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Hasegawa N, Hanaoka F, Yamada M, Increased unscheduled DNA synthesis in aged human diploid fibroblasts. Mech Ageing Dev25:297-305 1984
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Goldstein RH, Wall M, Activation of protein formation and cell division by bradykinin and des- Arg9-bradykinin. J Biol Chem259:9263-8 1984
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Absher M, Cristofalo VJ, Analysis of cell division by time-lapse cinematographic studies of hydrocortisone-treated embryonic lung fibroblasts. J Cell Physiol119:315-9 1984
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Sariban E, Erickson LC, Kohn KW, Effects of carbamoylation on cell survival and DNA repair in normal human embryo cells (IMR-90) treated with various 1-(2-chloroethyl)-1- nitrosoureas. Cancer Res44:1352-7 1984
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Asselineau D, Bell E, Prolylhydroxylase activation in IMR 90 fibroblasts: state of differentiation rather than population doubling level determines requirements for enzyme activation. Mech Ageing Dev25:257-66 1984
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Straus DS, Pang KJ, Effects of bradykinin on DNA synthesis in resting NIL8 hamster cells and human fibroblasts. Exp Cell Res151:87-95 1984
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Yoshikura H, Nishida J, Yoshida M, Kitamura Y, Takaku F, Ikeda S, Isolation of HTLV derived from Japanese adult T-cell leukemia patients in human diploid fibroblast strain IMR90 and the biological characters of the infected cells. Int J Cancer33(6):745-9 1984
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Balin AK, Fisher AJ, Carter DM, Oxygen modulates growth of human cells at physiologic partial pressures. J Exp Med160(1):152-66 1984
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Proia RL, d'Azzo A, Neufeld EF, Association of alpha- and beta-subunits during the biosynthesis of beta- hexosaminidase in cultured human fibroblasts. J Biol Chem259:3350-4 1984
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Stein GH, Human diploid fibroblasts (HDF) can induce DNA synthesis in cycling HDF but not in quiescent HDF or senescent HDF. Exp Cell Res144:468-71 1983
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Weissman BE, Stanbridge EJ, Complexity of control of tumorigenic expression in intraspecies hybrids of human SV40-transformed fibroblasts and normal human fibroblast cell lines. Cytogenet Cell Genet35:263-8 1983
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Wedel N, Toselli P, Pothoulakis C, Faris B, Oliver P, Franzblau C, LaMont T, Ultrastructural effects of Clostridium difficile toxin B on smooth muscle cells and fibroblasts. Exp Cell Res148:413-22 1983
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Roff CF, Wozniak RW, Blenis J, Wang JL, The effect of mannose6-phosphate on the turnover of cell surface glycosaminoglycans. Exp Cell Res144:333-44 1983
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Hildebran JN, Absher M, Low RB, Altered rates of collagen synthesis in in vitro aged human lung fibroblasts. In Vitro19:307-14 1983
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Bemiller P, Kraus ER Jr, The effect of clinical and subclinical concentrations of lead on the in vitro aging of human fibroblasts. Mech Ageing Dev22:79-87 1983
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Halton DM, Peterson WD Jr, Hukku B, Cell culture quality control by rapid isoenzymatic characterization. In Vitro19:16-24 1983
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Chung HR, Albanese EA, Studzinski GP, Differential effect of coformycin on the cell cycle traverse of normal and simian virus 40-transformed human fibroblasts. Cancer Res43:1269-74 1983
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Wilson VL, Jones PA, DNA methylation decreases in aging but not in immortal cells. Science220:1055-7 1983
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Backer JM, Bourret L, Dice JF, Regulation of catabolism of microinjected ribonuclease A requires the amino-terminal 20 amino acids. Proc Natl Acad Sci U S A80:2166-70 1983
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Absher M, Sylwester D, Hart BA, Time-lapse cinematographic analysis of beryllium--lung fibroblast interactions. Environ Res30:34-43 1983
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Becker D, Lane MA, Cooper GM, Identification of an antigen associated with transforming genes of human and mouse mammary carcinomas. Proc Natl Acad Sci U S A79:3315-9 1982
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Bannai S, Ishii T, Transport of cystine and cysteine and cell growth in cultured human diploid fibroblasts: effect of glutamate and homocysteate. J Cell Physiol112:265-72 1982
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Lubin M, Cahn F, Coutermarsh BA, Amiloride, protein synthesis, and activation of quiescent cells. J Cell Physiol113:247-51 1982
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Duthu GS, Braunschweiger KI, Pereira-Smith OM, Norwood TH, Smith JR, A long-lived human diploid fibroblast line for cellular aging studies: applications in cell hybridization. Mech Ageing Dev20:243-52 1982
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Ware JL, Paulson DF, Parks SF, Webb KS, Production of monoclonal antibody alpha Pro3 recognizing a human prostatic carcinoma antigen. Cancer Res42:1215-22 1982
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Anderson PJ, Actin and tubulin in normal and cystic fibrosis fibroblasts. Biochem Biophys Res Commun108:182-5 1982
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Hasegawa N, Hanaoka F, Hori T, Yamada M, Reevaluation of DNA chain elongation rate in human diploid fibroblasts. Exp Cell Res140:443-7 1982
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Stanbridge EJ, Rosen SW, Sussman HH, Expression of the alpha subunit of human chorionic gonadotropin is specifically correlated with tumorigenic expression in human cell hybrids. Proc Natl Acad Sci U S A79:6242-5 1982
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Torrelio BM, Paz MA, Gallop PM, Modulation of purine synthesis and phosphoribosylpyrophosphate content in human fibroblasts at different population doublings. Mech Ageing Dev19:147-58 1982
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Starling JJ, Sieg SM, Beckett ML, Schellhammer PF, Ladaga LE, Wright GL Jr, Monoclonal antibodies to human prostate and bladder tumor-associated antigens. Cancer Res42:3084-9 1982
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Smerdon MJ, Watkins JF, Lieberman MW, Effect of histone H1 removal on the distribution of ultraviolet-induced deoxyribonucleic acid repair synthesis within chromatin. Biochemistry21:3879-85 1982
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Sterling KM Jr, DiPetrillo TA, Kotch JP, Cutroneo KR, Bleomycin-induced increase of collagen turnover in IMR-90 fibroblasts: an in vitro model of connective tissue restructuring during lung fibrosis. Cancer Res42:3502-6 1982
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Stanbridge EJ, Der CJ, Doersen CJ, Nishimi RY, Peehl DM, Weissman BE, Wilkinson JE, Human cell hybrids: analysis of transformation and tumorigenicity. Science215:252-9 1982
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Carnicero HH, Englard S, Seifter S, Carnitine uptake and fatty acid utilization by diploid cells aging in culture. Arch Biochem Biophys215:78-88 1982
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Ballard FJ, Nield MK, Francis GL, Dahlenburg GW, Wallace JC, The relationship between the insulin content and inhibitory effects of bovine colostrum on protein breakdown in cultured cells. J Cell Physiol110(3):249-54 1982
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Lamon KD, Chiger JL, Kaji H, Effects of hydrocortisone and aspirin on protein synthesis and post-translational protein modification in cultured cells Biochem Pharmacol31(11):2047-52 1982
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Wilson GN, Szura LL, Rushford C, Jackson D, Erickson J, Structure and variation of human ribosomal DNA: the external transcribed spacer and adjacent regions. Am J Hum Genet34:32-49 1982
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Fong D, Poole B, The effect of canavanine on protein synthesis and protein degradation in IMR-90 fibroblasts. Biochim Biophys Acta696:193-200 1982
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Gallop PM, Paz MA, Henson E, Boradeption: a new procedure for transferring water-insoluble agents across cell membranes. Science217:166-9 1982
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Namba M, Fukushima F, Kimoto T, Effects of feeder layers made of human, mouse, hamster, and rat cells on the cloning efficiency of transformed human cells. In Vitro18:469-75 1982
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Vogel KG, Peterson DW, Extracellular, surface, and intracellular proteoglycans produced by human embryo lung fibroblasts in culture (IMR-90). J Biol Chem256:13235-42 1981
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Kelley J, Trombley L, Kovacs EJ, Davis GS, Absher M, Pulmonary macrophages alter the collagen phenotype of lung fibroblasts. J Cell Physiol109:353-61 1981
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Didinsky JB, Rheinwald JG, Failure of hydrocortisone or growth factors to influence the senescence of fibroblasts in a new culture system for assessing replicative lifespan. J Cell Physiol109:171-9 1981
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Wu RS, Bonner WM, Separation of basal histone synthesis from S-phase histone synthesis in dividing cells. Cell27:321-30 1981
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Imai K, Galloway DR, Ferrone S, Serological and immunochemical analysis of the specificity of xenoantiserum 8986 elicited with hybrids between human melanoma cells and murine fibroblasts. Cancer Res41:1028-33 1981
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Der CJ, Stanbridge EJ, A tumor-specific membrane phosphoprotein marker in human cell hybrids. Cell26:429-38 1981
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Stein GH, Yanishevsky RM, Quiescent human diploid cells can inhibit entry into S phase in replicative nuclei in heterodikaryons. Proc Natl Acad Sci U S A78:3025-9 1981
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Nohga K, Reddy R, Busch H, Comparison of RNase T1 fingerprints of U1, U2, and U3 small nuclear RNA's of HeLa cells, human normal fibroblasts, and Novikoff hepatoma cells. Cancer Res41:2215-20 1981
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Fox N, Fernandez C, Studzinski GP, Visualization of nucleolar substructure in cultured human fibroblasts by magnesium-activated adenosine triphosphatase reaction. J Histochem Cytochem29:1115-20 1981
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Carnicero HH, Adamany AM, Englard S, Collagen glucosyl- and galactosyltransferases of cultured human fetal lung fibroblasts. Arch Biochem Biophys210:678-90 1981
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Vogel KG, Kendall VF, Sapien RE, Glycosaminoglycan synthesis and composition in human fibroblasts during in vitro cellular aging (IMR-90). J Cell Physiol107:271-81 1981
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Bradley MO, Taylor VI, DNA double-strand breaks induced in normal human cells during the repair of ultraviolet light damage. Proc Natl Acad Sci U S A78:3619-23 1981
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Morgan AC Jr, Galloway DR, Jensen FC, Giovanella BC, Reisfeld RA, Immunochemical delineation of an oncofetal antigen on normal and simian virus 40-transformed human fetal melanocytes. Proc Natl Acad Sci U S A78:3834-8 1981
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Paz MA, Torrelio M, Gallop PM, X-linked processes in serially passaged "aging" human diploid cells. J Gerontol36:142-51 1981
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Wu BC, Spohn WH, Busch H, Comparison of nuclear proteins of several human tumors and normal cells by two-dimensional gel electrophoresis. Cancer Res41:336-42 1981
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McCurry LS, Jacobson MK, Poly(ADP-ribose) synthesis following DNA damage in cells heterozygous or homozygous for the xeroderma pigmentosum genotype. J Biol Chem256:551-3 1981
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Gonnerman WA, Ferrera R, Franzblau C, Measurement of medium lysyl oxidase activity in aorta smooth muscle cells. Effects of multiple medium changes and inhibition of protein synthesis. Biochemistry20:3864-7 1981
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James L, Veomett GE, Decreased longevity of human diploid cells after incorporation of latex spheres within their cytoplasm. Exp Cell Res132:468-73 1981
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Hara H, Lamon KD, Kaji H, Effect of temperature on normal and SV40-transformed human fibroblasts. Biochim Biophys Acta673(1):37-45 1981
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Neff NT, Bourret L, Miao P, Dice JF, Degradation of proteins microinjected into IMR-90 human diploid fibroblasts. J Cell Biol91:184-94 1981
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Ochs DC, McConkey EH, Guard NL, Vimentin-derived proteins: differences between normal human fibroblasts and transformed human cells. Exp Cell Res135:355-62 1981
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Bourgeois CA, Raynaud N, Diatloff-Zito C, Macieira-Coehlo A, Effect of low dose rate ionizing radiation on the division potential of cells in vitro. VIII. Cytogenetic analysis of human fibroblasts. Mech Ageing Dev17:225-35 1981
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Taylor L, Schneider E, Smith J, Polgar P, Prostaglandin production and cellular aging. Mech Ageing Dev16:311-7 1981
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Vogel KG, Kelley RO, Stewart C, Loss of organized fibronectin matrix from the surface of aging diploid fibroblasts (IMR-90). Mech Ageing Dev16:295-302 1981
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Sarber R, Hull B, Merrill C, Soranno T, Bell E, Regulation of proliferation of fibroblasts of low and high population doubling levels grown in collagen lattices. Mech Ageing Dev17:107-17 1981
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Hennis HL 3d, Braid HL, Vincent RA Jr, Unscheduled DNA synthesis in cells of different shape in fibroblast cultures from donors of various ages. Mech Ageing Dev16:355-61 1981
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Lechner JF, Haugen A, Autrup H, McClendon IA, Trump BF, Harris CC, Clonal growth of epithelial cells from normal adult human bronchus. Cancer Res41:2294-304 1981
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Pool TB, Heitman TO, Buck MA, Changes in nuclear shape and mitochondrial structure do not accompany the loss of division potential in human fibroblasts in vitro. Am J Anat162:369-82 1981
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Lane MA, Sainten A, Cooper GM, Activation of related transforming genes in mouse and human mammary carcinomas. Proc Natl Acad Sci U S A78:5185-9 1981
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View karyotype 
PDL at Senescence 62
Cumulative PDL at Freeze 19
Passage Frozen 5
Split Ratio 1:5
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids
Serum 15% fetal bovine serum Not inactivated
Substrate None specified
Subcultivation Method trypsin-EDTA