NA11418
DNA from Somatic cell hybrid
Description:
NIGMS MAPPING PANEL #2, VERSION 3, HUMAN/RODENT SOMATIC CELL HYBRIDS
NIGMS HUMAN/RODENT SOMATIC CELL HYBRID MAPPING PANEL #2 DNA
NIGMS HUMAN/RODENT SOMATIC CELL HYBRID MINI MAPPING PANEL #2 DNA
Repository
|
NIGMS Human Genetic Cell Repository
|
Subcollection |
Hybrids |
Quantity |
0.050mg |
Quantitation Method |
Please see our FAQ |
Cell Type
|
Somatic cell hybrid
|
Transformant
|
Untransformed
|
Sample Source
|
DNA from Somatic cell hybrid
|
Confirmation
|
Karyotypic analysis and Southern blot hybridization
|
ISCN
|
Human/Chinese hamster somatic cell hybrid retaining human chromosome #15
|
Remarks
|
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
|
GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for Xp22.32 and Yp11.3, MIC2, and Xq28, DXS15. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for Xp22.32, STS, Xq13.3, PGK1, and Xq28, G6PD. |
|
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 1p13, NGFB, and 1q32, REN. |
|
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 2p13, TGFA, 2p23, POMC, and 2q13, IL1A. PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 2q37, ALPP. |
|
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 3p22-p21.33, GLB1, and 3q26.2-q27, GLUT2. |
|
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 4p15.3, QDPR, and 4q11-q13, AFP. |
|
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 5p14-p12, C9, and 5q13.3-q14, HMGCR. |
|
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 6 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 6p21.3, TNFA, and 6q24-q27, MAS1. |
|
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 7 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 7p12, EGFR, and 7q31.3, CFTR. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 7pter-p22, D7S472, and 7q31.3, CFTR. |
|
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 8 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 8p21-p11.2, LHRH, 8p23-p22, DEF1, and 8q24, TG. |
|
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 9 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 9p22, IFNB, and 9q34.1, ABL. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 9p22, IFNA, and 9q22.3-q31, ALDOB. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 9p21, D9S270, 9pter-p22, D9S178, and 9pter-q12, RLN1. |
|
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 10 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 10p15p14, IL2R, and 10q24qter, PLAU. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 10p11.2, ITGB1, and 10q24, PLAU. |
|
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 11p15.5, D11S12, and 11q23.3, ETS1. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 11p13, CAT, and 11q21-q22, CLG. |
|
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 12 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 12p13.3-p13.2, F8VWF, and 12q22-q24.2, PAH. |
|
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 13 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 13q34, D13S3. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 13q14.3, RB1. |
|
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 14 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 14q11.2, NP, and 14q32.1, PI. |
|
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 15 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 15q25qter, IGF1R. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 15q21-q22.2, B2M. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 15q11-q12, D15S11, and 15q11.2-q12, GABRB3.. |
|
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 16p13.3, HBA, 16q22.1, HPR, and 16q22.1, LCAT. |
|
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 17p13.1, TP53, 17q, GAS, and 17q21.3-q23, MPO. |
|
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 18q23, MBP, and 18q12.3, D18S6. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 18p11.31-p11.22, TYMS. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 18p11.1-q11.2, D18S44, and 18q12.1, TTR. |
|
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 19 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 19p13.2, LDLR, and 19q13.2, APOC2. |
|
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 20 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 20p12, D20S6, and 20q13.2, D20S4. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 20pter-p12, PRNP, and 20q12-q13.11, ADA. |
|
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 21q22.3, ITGB2. |
|
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 22q11.2, IGLC2. |
|
Remarks |
Line 15A; produced by the fusion of human acute myelogenous leukemia cells and Chinese hamster ovary line CHO-K1; retains human chromosome #15 in 80% of cells; 100% of cells had 2 or 3 different nonrandom human/rodent translocation chromosomes |
Thameem F, Puppala S, Arar NH, Blangero J, Duggirala R, Abboud HE, Genetic variants in transient receptor potential cation channel, subfamily M 1 (TRPM1) and their risk of albuminuria-related traits in Mexican Americans Clinica chimica acta; international journal of clinical chemistry412:2058-62 2010 |
PubMed ID: 21439949 |
|
Krona C, Ejeskär K, Carén H, Abel F, Sjöberg RM, Martinsson T, A novel 1p362 located gene, APITD1, with tumour-suppressive properties and a putative p53-binding domain, shows low expression in neuroblastoma tumours British journal of cancer91:1119-30 2004 |
PubMed ID: 15328517 |
|
Mallet F, Bouton O, Prudhomme S, Cheynet V, Oriol G, Bonnaud B, Lucotte G, Duret L, Mandrand B, The endogenous retroviral locus ERVWE1 is a bona fide gene involved in hominoid placental physiology Proceedings of the National Academy of Sciences of the United States of America101:1731-6 2004 |
PubMed ID: 14757826 |
|
Baccetti B, Bruni E, Collodel G, Gambera L, Moretti E, Marzella R, Piomboni P, 10, 15 reciprocal translocation in an infertile man: ultrastructural and fluorescence in-situ hybridization sperm study: case report Human reproduction (Oxford, England)18:2302-8 2003 |
PubMed ID: 14585878 |
|
Conte C, Ebeling M, Marcuz A, Nef P, Andres-Barquin PJ, Identification and characterization of human taste receptor genes belonging to the TAS2R family Cytogenetic and genome research98:45-53 2003 |
PubMed ID: 12584440 |
|
Hansford LM, Smith SA, Haber M, Norris MD, Cheung B, Marshall GM, Cloning and characterization of the human neural cell adhesion molecule, CNTN4 (alias BIG-2) Cytogenetic and genome research101:17-23 2003 |
PubMed ID: 14571131 |
|
Janssens B, Mohapatra B, Vatta M, Goossens S, Vanpoucke G, Kools P, Montoye T, van Hengel J, Bowles NE, van Roy F, Towbin JA, Assessment of the CTNNA3 gene encoding human alpha T-catenin regarding its involvement in dilated cardiomyopathy Human genetics112:227-36 2003 |
PubMed ID: 12596047 |
|
Tarafa G, Prat E, Risques RA, González S, Camps J, Grau M, Guinó E, Moreno V, Esteller M, Herman JG, Germà JR, Miró R, Peinado MA, Capellá G, Common genetic evolutionary pathways in familial adenomatous polyposis tumors Cancer research63:5731-7 2003 |
PubMed ID: 14522893 |
|
Trask BJ, Friedman C, Martin-Gallardo A, Rowen L, Akinbami C, Blankenship J, Collins C, Giorgi D, Iadonato S, Johnson F, Kuo WL, Massa H, Morrish T, Naylor S, Nguyen OT, Rouquier S, Smith T, Wong DJ, Youngblom J, van den Engh G, Members of the olfactory receptor gene family are contained in large blocks of DNA duplicated polymorphically near the ends of human chromosomes. Hum Mol Genet7:13-26 1998 |
PubMed ID: 9384599 |
|
Dyomin VG, Rao PH, Dalla-Favera R, Chaganti RS, BCL8, a novel gene involved in translocations affecting band 15q11-13 in diffuse large-cell lymphoma. Proc Natl Acad Sci U S A94(11):5728-32 1997 |
PubMed ID: 9159141 |
|
McDaniel LD, Zhang B, Kubiczek E, Ritter M, Huang J, Berard C, Leana-Cox J, Schwartz S, Schultz RA, Construction and screening of a cosmid library generated from a somatic cell hybrid bearing human chromosome 15. Genomics40:63-72 1997 |
PubMed ID: 9070920 |
|
Schwarz H, Arden K, Lotz M, CD137, a member of the tumor necrosis factor receptor family, is located on chromosome 1p36, in a cluster of related genes, and colocalizes with several malignancies Biochemical and biophysical research communications235:699-703 1997 |
PubMed ID: 9207223 |
|
Finelli P, Antonacci R, Marzella R, Lonoce A, Archidiacono N, Rocchi M, Structural organization of multiple alphoid subsets coexisting on human chromosomes 1, 4, 5, 7, 9, 15, 18, and 19. Genomics38:325-30 1996 |
PubMed ID: 8975709 |
|
Hirai M, Kusuda J, Hashimoto K, Assignment of human ADP ribosylation factor (ARF) genes ARF1 and ARF3 to chromosomes 1q42 and 12q13, respectively. Genomics34(2):263-5 1996 |
PubMed ID: 8661066 |
|
Argeson AC, Druck T, Veronese ML, Knopf JL, Buchberg AM, Huebner K, Siracusa LD, Phospholipase C gamma-2 (Plcg2) and phospholipase C gamma-1 (Plcg1) map to distinct regions in the human and mouse genomes. Genomics25:29-35 1995 |
PubMed ID: 7774933 |
|
Gimbel W, Klein V, Brass N, Fischer U, Piontek K, Overmyer K, Gottert E, Zang KD, Meese E, Isolation and localization of transcribed sequences on human chromosome 22. Cytogenet Cell Genet71:81-5 1995 |
PubMed ID: 7606934 |
|
Hobson GM, Krahe R, Garcia E, Siciliano MJ, Funanage VL, Regional chromosomal assignments for four members of the MADS domain transcription enhancer factor 2 (MEF2) gene family to human chromosomes 15q26, 19p12, 5q14, and 1q12-q23. Genomics29:704-11 1995 |
PubMed ID: 8575763 |
|
Kermouni A, Van Roost E, Arden KC, Vermeesch JR, Weiss S, Godelaine D, Flint J, Lurquin C, Szikora JP, Higgs DR, The IL-9 receptor gene (IL9R): genomic structure, chromosomal localization in the pseudoautosomal region of the long arm of the sex chromosomes, and identification of IL9R pseudogenes at 9qter, 10pter, 16pter, and 18pter Genomics29:371-82 1995 |
PubMed ID: 8666384 |
|
Lyle R, Wright TJ, Clark LN, Hewitt JE, The FSHD-associated repeat, D4Z4, is a member of a dispersed family of homeobox-containing repeats, subsets of which are clustered on the short arms of the acrocentric chromosomes. Genomics28:389-97 1995 |
PubMed ID: 7490072 |
|
Sakai K, Ohta T, Minoshima S, Kudoh J, Wang Y, de Jong PJ, Shimizu N, Human ribosomal RNA gene cluster: identification of the proximal end containing a novel tandem repeat sequence. Genomics26:521-6 1995 |
PubMed ID: 7607675 |
|
Brass N, Fischer U, Mueller HW, Klein V, Meese E, Strategy for chromosomal assignment of expressed sequences derived from heteronuclear RNA. Biotechniques17:88-90, 92 1994 |
PubMed ID: 7946323 |
|
Chang DY, Nelson B, Bilyeu T, Hsu K, Darlington GJ, Maraia RJ, A human Alu RNA-binding protein whose expression is associated with accumulation of small cytoplasmic Alu RNA. Mol Cell Biol14:3949-59 1994 |
PubMed ID: 8196634 |
|
Fougerousse F, Broux O, Richard I, Allamand V, de Souza AP, Bourg N, Brenguier L, Devaud C, Pasturaud P, Roudaut C, Mapping of a chromosome 15 region involved in limb girdle muscular dystrophy Human molecular genetics3:285-93 1994 |
PubMed ID: 8004096 |
|
Fukushima A, Okubo K, Sugino H, Hori N, Matoba R, Niiyama T, Murakawa K, Yoshii J, Yokoyama M, Matsubara K, Chromosomal assignment of HepG2 3'-directed partial cDNA sequences by Southern blot hybridization using monochromosomal hybrid cell panels. Genomics22:127-36 1994 |
PubMed ID: 7959758 |
|
Maraia RJ, Sasaki-Tozawa N, Driscoll CT, Green ED, Darlington GJ, The human Y4 small cytoplasmic RNA gene is controlled by upstream elements and resides on chromosome 7 with all other hY scRNA genes Nucleic acids research22:3045-52 1994 |
PubMed ID: 7520568 |
|
Nagaoka H, Ozawa K, Matsuda F, Hayashida H, Matsumura R, Haino M, Shin EK, Fukita Y, Imai T, Anand R, et al, Recent translocation of variable and diversity segments of the human immunoglobulin heavy chain from chromosome 14 to chromosomes 15 and 16. Genomics22:189-97 1994 |
PubMed ID: 7959766 |
|
Richard I, Broux O, Chiannilkulchai N, Fougerousse F, Allamand V, Bourg N, Brenguier L, Devaud C, Pasturaud P, Roudaut C, Regional localization of human chromosome 15 loci Genomics23:619-27 1994 |
PubMed ID: 7851890 |
|
Chong SS, Kristjansson K, Zoghbi HY, Hughes MR, Molecular cloning of the cDNA encoding a human renal sodium phosphate transport protein and its assignment to chromosome 6p21.3-p23. Genomics18:355-359 1993 |
PubMed ID: 8288239 |
|
Clark CC, Cohen I, Eichstetter I, Cannizzaro LA, McPherson JD, Wasmuth JJ, Iozzo RV, Molecular cloning of the human proto-oncogene Wnt-5A and mapping of the gene (WNT5A) to chromosome 3p14-p21. Genomics18:249-60 1993 |
PubMed ID: 8288227 |
|
Dittrich B, Buiting K, Gross S, Horsthemke B, Characterization of a methylation imprint in the Prader-Willi syndrome chromosome region. Hum Mol Genet2:1995-9 1993 |
PubMed ID: 8111366 |
|
Drwinga HL, Toji LH, Kim CH, Greene AE, Mulivor RA, NIGMS human/rodent somatic cell hybrid mapping panels 1 and 2. Genomics16:311-4 1993 |
PubMed ID: 8314568 |
|
Howe JR, Lairmore TC, Veile R, Dou S, Wells SA Jr, Donis-Keller H, Development of a sequence-tagged site for the centromere of chromosome 10: its use in cytogenetic and physical mapping. Hum Genet91:199-204 1993 |
PubMed ID: 8478002 |
|
Tsuda T, Cutler ML, Human RSU1 is highly homologous to mouse Rsu-1 and localizes to human chromosome 10. Genomics18:461-2 1993 |
PubMed ID: 8288261 |
|
Tasset DM, Hartz JA, Kao FT, Isolation and analysis of DNA markers specific to human chromosome 15. Am J Hum Genet42:854-66 1988 |
PubMed ID: 2897161 |
|
|