NA12513
DNA from Somatic cell hybrid
Description:
CHROMOSOME 17 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
Repository
|
NIGMS Human Genetic Cell Repository
|
Subcollection |
Hybrids |
Quantity |
0.050mg |
Quantitation Method |
Please see our FAQ |
Cell Type
|
Somatic cell hybrid
|
Transformant
|
Untransformed
|
Sample Source
|
DNA from Somatic cell hybrid
|
Confirmation
|
Clinical summary/Case history
|
ISCN
|
Mouse/human hybrid retaining a human del(17)(p13.3)
|
Remarks
|
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
|
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 17p13.1, TP53, 17q21.3-q23, MPO1, 17q, GAS, and 17q25-qter, D17S784. |
|
Remarks |
Line KCB4; produced by the fusion of human cells from a Miller-Dieker syndrome pt, del(17)(p13.3), with TK-deficient mouse Cl1D cells; grown in HAT medium; retains human del(17) in 96% of cells |
Ledbetter SA, Kuwano A, Dobyns WB, Ledbetter DH, Microdeletions of chromosome 17p13 as a cause of isolated lissencephaly. Am J Hum Genet50:182-9 1992 |
PubMed ID: 1346078 |
|
|