NG01520
DNA from Fibroblast
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
| Quantity |
10 µg |
| Quantitation Method |
Please see our FAQ |
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Biopsy Source
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Foreskin
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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DNA from Fibroblast
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Relation to Proband
|
proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Senescence |
47 |
| PDL at Freeze |
12 |
| Passage Frozen |
6 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| Remarks |
The culture was initiated on 1/21/76 from explants of minced foreskin removed ante-mortem 1 day earlier. The cell morphology is fibroblast-like. The karyotype is 46,XY; normal diploid male. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Gailit J, Pierschbacher M, Clark RA, Expression of functional alpha 4 beta 1 integrin by human dermal fibroblasts. J Invest Dermatol100:323-8 1993 |
| PubMed ID: 8440915 |
| |
| Stokes DG, Saitta B, Timpl R, Chu ML, Human alpha 3(VI) collagen gene. Characterization of exons coding for the amino-terminal globular domain and alternative splicing in normal and tumor cells. J Biol Chem266:8626-33 1991 |
| PubMed ID: 2022673 |
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| Nove J, Little JB, Mayer PJ, Troilo P, Nichols WW, Hypersensitivity of cells from a new chromosomal-breakage syndrome to DNA-damaging agents. Mutat Res163:255-62 1986 |
| PubMed ID: 3785261 |
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| Sephel GC, Davidson JM, Elastin production in human skin fibroblast cultures and its decline with age. J Invest Dermatol86:279-85 1986 |
| PubMed ID: 3745952 |
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