NG07135
DNA from Fibroblast
Description:
GERONTOLOGY RESEARCH CENTER (GRC) CELL CULTURE COLLECTION
BALTIMORE LONGITUDINAL STUDY ON AGING (BLSA)
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
GRC |
| Quantity |
10 µg |
| Quantitation Method |
Please see our FAQ |
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Biopsy Source
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Arm
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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DNA from Fibroblast
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Race
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White
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY,13s+
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
17 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
The biopsy site is the mesial aspect of the mid-upper left arm, taken antemortem. The primary culture was initiated from explants of a 2 mm-punch biopsy. The cell morphology is fibroblast-like and the karyotype is 46,XY,13s+. Cultures initiated from biopsies taken 3, 7, and 13 years later from this same donor are AG07143, AG11488, and AG12788, respectively. Donor's daughter is AG12786. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Barroso-Vilares M, Macedo JC, Reis M, Warren JD, Compton D, Logarinho E, Small-molecule inhibition of aging-associated chromosomal instability delays cellular senescence EMBO reports:e49248 2019 |
| PubMed ID: 32134180 |
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| Miyoshi N, Oubrahim H, Chock PB, Stadtman ER, Age-dependent cell death and the role of ATP in hydrogen peroxide-induced apoptosis and necrosis Proceedings of the National Academy of Sciences of the United States of America103:1727-31 2006 |
| PubMed ID: 16443681 |
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| Greco M, Villani G, Mazzucchelli F, Bresolin N, Papa S, Attardi G, Marked aging-related decline in efficiency of oxidative phosphorylation in human skin fibroblasts The FASEB journal : official publication of the Federation of American Societies for Experimental Biology17:1706-8 2003 |
| PubMed ID: 12958183 |
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| Zhang J, Asin-Cayuela J, Fish J, Michikawa Y, Bonafe M, Olivieri F, Passarino G, De Benedictis G, Franceschi C, Attardi G, Strikingly higher frequency in centenarians and twins of mtDNA mutation causing remodeling of replication origin in leukocytes. Proc Natl Acad Sci U S A100(3):1116-21 2003 |
| PubMed ID: 12538859 |
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