NG11545
DNA from Smooth muscle
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
| Quantity |
10 µg |
| Quantitation Method |
Please see our FAQ |
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Biopsy Source
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Artery, iliac
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Cell Type
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Smooth muscle
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Tissue Type
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Artery
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Transformant
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Untransformed
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Sample Source
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DNA from Smooth muscle
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
24 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Line HIAS-117C. The iliac artery was removed from a braindead, heartbeating cadaver, organ donor on 11/16/88. The donor died from a motor vehicle accident and had no known diseases. The culture was initiated by explantation of tissue dissected from the medial layer. The culture tested positive for alpha-smooth muscle actin (immunofluorescence assay). The culture was grown on gelatin-coated flasks, and the medium was supplemented with 0.02 mg/ml ECGS and 0.05 mg/ml heparin. Cells were frozen in growth medium containing 7.5% polyvinylpyrrolidinone and 5% DMSO. |
| Mesri M, Wall NR, Li J, Kim RW, Altieri DC, Cancer gene therapy using a survivin mutant adenovirus. J Clin Invest108(7):981-90 2001 |
| PubMed ID: 11581299 |
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