GM23762
iPSC from Fibroblast
Description:
SCHIZOPHRENIA; SCZD
Repository
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NIGMS Human Genetic Cell Repository
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Subcollection |
Heritable Diseases |
Protocols |
Protocol PDF |
Biopsy Source
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Skin
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Cell Type
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Stem cell
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Cell Subtype
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Induced pluripotent stem cell
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Transformant
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Reprogrammed (Lentiviral)
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Sample Source
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iPSC from Fibroblast
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Race
|
White
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Ethnicity
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JEWISH
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Family Member
|
1
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Relation to Proband
|
proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY,del(19)(q13.2)[1]/46,XY[24]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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Passage Frozen |
26 |
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Induced Pluripotent Stem Cell |
The cell line submitted to the Repository frozen was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation and the PluriTest. Steady-state mRNA expression patterns of undifferentiated iPSCs and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis. |
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Remarks |
Induced pluripotent stem cell culture derived from GM02497 Fibroblast; see GM01488 Lymphoid; paralogical thinking, affective shielding, splitting of affect from content, and suspiciousness; onset at age 15; hospitalized; positive family history. Researchers purchasing hiPSCs from the NIGMS Repository are responsible for any limited use label licenses (LULLs) applicable to the cell line purchased. The applicable LULL to this line is iPS Academia, Japan Inc.. |
Passage Frozen |
26 |
Split Ratio |
1:6 |
Temperature |
37 C |
Percent CO2 |
5% |
Percent O2 |
AMBIENT |
Medium |
Knockout DMEM |
Serum |
20% Knock-out Serum Replacement |
Substrate |
Gelatin + Feeder Layer |
Supplement |
Basic Fibroblast Growth Factor 5 mg/L |
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