AG07090
Mesothelial from Ascites fluid
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Ascites fluid
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Cell Type
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Mesothelial
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Tissue Type
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Peritoneum
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Transformant
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Untransformed
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Sample Source
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Mesothelial from Ascites fluid
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
4.59 |
| Passage Frozen |
11 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Normal human mesothelial cell strain LP-3. This culture was initiated on 10/1/83 by directly plating an ascites fluid cell suspension which had been stored frozen since removal from the peritoneal cavity of an ovarian cancer patient on 9/19/80. The culture medium must be supplemented with 10 nanograms/ml EGF and 0.4 micrograms/ml hydrocortisone. The cell morphology is fibroblast-like in medium supplemented with EGF, but is epithelial-like in medium without EGF. The karyotype is 46,XX; normal diploid female. **This line will only be shipped frozen.** The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Ren J, Xiao YJ, Singh LS, Zhao X, Zhao Z, Feng L, Rose TM, Prestwich GD, Xu Y, Lysophosphatidic acid is constitutively produced by human peritoneal mesothelial cells and enhances adhesion, migration, and invasion of ovarian cancer cells Cancer research66:3006-14 2006 |
| PubMed ID: 16540649 |
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| Kanerva A, Wang M, Bauerschmitz GJ, Lam JT, Desmond RA, Bhoola SM, Barnes MN, Alvarez RD, Siegal GP, Curiel DT, Hemminki A, Gene transfer to ovarian cancer versus normal tissues with fiber-modified adenoviruses. Mol Ther5(6):695-704 2002 |
| PubMed ID: 12027553 |
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| Casado E, Gomez-Navarro J, Yamamoto M, Adachi Y, Coolidge CJ, Arafat WO, Barker SD, Wang MH, Mahasreshti PJ, Hemminki A, Gonzalez-Baron M, Barnes MN, Pustilnik TB, Siegal GP, Alvarez RD, Curiel DT, Strategies to accomplish targeted expression of transgenes in ovarian cancer for molecular therapeutic applications. Clin Cancer Res7(8):2496-504 2001 |
| PubMed ID: 11489832 |
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| Lee TC, Zhang Y, Aston C, Hintz R, Jagirdar J, Perle MA, Burt M, Rom WN, Normal human mesothelial cells and mesothelioma cell lines express insulin-like growth factor I and associated molecules. Cancer Res53:2858-64 1993 |
| PubMed ID: 7684950 |
| Cumulative PDL at Freeze |
29.59 |
| Passage Frozen |
11 |
| Split Ratio |
1:6 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Medium 199 in Earle BSS/MCDB110, 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Supplement |
Epidermal Growth Factor 10 ng/ml |
| Supplement |
Hydrocortisone 0.4 ug/ml |
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