AG11131
Epithelial from Breast organoid
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Breast organoid
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Cell Type
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Epithelial
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Tissue Type
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Breast
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Transformant
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Untransformed
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Sample Source
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Epithelial from Breast organoid
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Senescence |
71 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Tissue obtained in 1978 during surgery for bilateral gynecomastia. Diagnosis was mild duct epithelial proliferation. Mammary epithelial clumps or organoids were isolated by tissue dissection followed by partial enzymatic digestion with collagenase and hyaluronidase. Epithelial organoids were separated from the digested stromal elements by filtration and stored in liquid nitrogen. The culture was initiated 7/88 from thawed organoids by explanation in serum-free MCDB 170 plus supplements. Serial subcultivation resulted in the selection of a population of proliferative epithelial cells. Culture submitted as line 40GL/AC209 at passage 6, PDL 25 (estimated by submitter), and was frozen at the Repository at PDL 35. Culture is grown in serum-free medium. |
| Stampfer, Isolation and growth of human mammary epithelial cells. J Tissue Culture Methods9:107 (1985): 1985 |
| PubMed ID: |
| PDL at Senescence |
71 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
MEGM-mammary epithelial cell medium plus supplements |
| Serum |
0% fetal bovine serum |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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