AG11136
Epithelial from Breast organoid
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Breast organoid
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Cell Type
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Epithelial
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Tissue Type
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Breast
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Transformant
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Untransformed
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Sample Source
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Epithelial from Breast organoid
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Tissue was obtained in 1981 during subcutaneous mastectomy of breast contralateral to breast removed for tumor; pathologic diagnosis was mild fibrocystic disease. Mammary epithelial clumps or organoids were isolated by tissue dissection followed by partial enzymatic digestion with collagenase and hyaluronidase. Epithelial organoids were separated from the digested stromal elements by filtration and stored in liquid nitrogen. The culture was initiated 2/85 from thawed organoids by explantation in serum-free MCDB 170 plus supplements. Serial subcultivation resulted in the selection of a population of proliferative epithelial cells. Culture submitted as line 210C/AB470 at passage 5, PDL 22 (estimated by submitter), and has been frozen at the Repository at PDL 30. Culture is grown in serumfree medium. THIS SAMPLE WILL ONLY BE SHIPPED FROZEN |
| Stampfer, Isolation and growth of human mammary epithelial cells. J Tissue Culture Methods9:107 (1985): 1985 |
| PubMed ID: |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
MEGM-mammary epithelial cell medium plus supplements |
| Serum |
0% fetal bovine serum |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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