AG11180
Endothelial from Artery, iliac
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Artery, iliac
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Cell Type
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Endothelial
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Tissue Type
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Artery
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Transformant
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Untransformed
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Sample Source
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Endothelial from Artery, iliac
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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74-89,XX,-X,-1,-4,-4,-7,-9,-11,-13,-13,-15,-16,-19,-20,-21,+1~7mar[cp10]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
24 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Line HIAE-32. The iliac artery was removed from a braindead, heartbeating cadaver, organ donor. The donor died of trauma and had no known diseases. The culture was initiated using cells released from the lumen of the vessel by collagenase digestion. Cell morphology is polygonal. The culture was grown on gelatin-coated flasks and the medium was supplemented with 0.02 mg/ml ECGS and 0.05 mg/ml heparin. Cells were frozen in growth medium containing 7.5% polyvinylpyrrolidinone and 5% DMSO. This culture tested positive for the endothelial cell-specific von Willebrand factor and negative for alpha-smooth muscle actin (immunofluorescence assays). Karyotype is unstable. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Nichols WW, Buynak EB, Bradt C, Hill R, Aronson M, Jarrell BE, Mueller SN, Levine EM, Cytogenetic evaluation of human endothelial cell cultures. J Cell Physiol132:453-62 1987 |
| PubMed ID: 3654763 |
| Cumulative PDL at Freeze |
24 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Medium 199 in Earl BSS with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Endothelial Cell Growth Supplement 0.02 mg/ml |
| Supplement |
Heparin 0.05 mg/ml |
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