AG16104
Fibroblast from Skin, Arm
Description:
APPARENTLY HEALTHY INDIVIDUAL
GERONTOLOGY RESEARCH CENTER (GRC) CELL CULTURE COLLECTION
INVERTED CHROMOSOME
BALTIMORE LONGITUDINAL STUDY ON AGING (BLSA)
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
GRC
Apparently Healthy Collection |
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Biopsy Source
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Arm
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Arm
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Race
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Black/African American
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XX,inv(9)(pter>p11::q13>p11::q13>qter)
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
6 |
| Passage Frozen |
4 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
The biopsy site is the mesial aspect of the mid-upper arm, taken ante-mortem. The primary culture was initiated from explants of a 2 mm-punch biopsy. The cell morphology is fibroblast-like and the karyotype is 46,XX,inv(9)(pter>p11::q13>p11::q13>qter) with 4% of the cells examined showing random chromosome gain.. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Cumulative PDL at Freeze |
6 |
| Passage Frozen |
4 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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