AG16273
Fibroblast from Whole embryo, Embryo
Description:
CRICETULUS
ORDER: RODENTIA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Embryo
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Cell Type
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Fibroblast
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Tissue Type
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Whole embryo
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Whole embryo, Embryo
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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22,XX[30]/23,XX,+6[20]
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Species
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Cricetulus griseus
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Common Name
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chinese hamster
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Genbank |
COX1 |
| Remarks |
Chinese hamster inbred strain. The biopsy was obtained by mincing a whole 8cm embryo. Cell morphology is fibroblast-like and the cells grow to form confluent sheets. The karyotype is 22,XX[30]/23,XX,+6[20] with 4% of the cells examined showing random chromosome loss/gain. A spontaneously transformed male fibroblast cell line, GM00458, is available from the NIGMS Human Genetic Cell Repository. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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