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CH00064
Plasmid
Description:
HTT-Q23-FRT, RDM, 1-3144, HUMAN
HUNTINGTON DISEASE; HD
Affected:
No Data
Sex:
No Data
Age:
No Data
Sample Description
Overview
Characterizations
Phenotypic Data
External Links
Culture Protocols
Overview
Repository
HD Community Biorepository
Quantity
20 µg
Quantitation Method
Please see our
FAQ
Certain Third Party Licensing Requirements
Invitrogen License for Cat# V6010-20
Alias
FLrdmQ23f
Sample Source
CHDI Foundation, Inc.
Species
Homo
sapiens
Common Name
Human
Remarks
DNA corresponding to the full length human sequence of the Huntingtin (Htt) gene containing 23 polyglutamine repeats translated from a random codon. rdm: x/CAG/CAA/x
Characterizations
Remarks
DNA corresponding to the full length human sequence of the Huntingtin (Htt) gene containing 23 polyglutamine repeats translated from a random codon. rdm: x/CAG/CAA/x
Construct Name
FLrdmQ23f
Insert Length
9454
Vector
pcDNA5/FRT
Concentration
75 ng/ml
Sequence
GNTAAACACCACCATGGCGACCCTGGAAAAGCTGATGAAGGCCTTCGAGTCCCTCAAGT
CCTTCCAGCAGCAGCAGCAGCAACAGCAGCAACAGCAGCAGCAACAACAGCAGCAGCAG
CAACAACAACAGCAGCCGCCACCGCCGCCGCCGCCGCCGCCGCCTCCTCAGCTTCCTCA
GCCGCCGCCGCAGGCACAGCCGCTGCTGCCTCAGCCGCAGCCGCCCCCGCCGCCGCCCC
CGCCGCCACCCGGCCCGGCTGTGGCTGAGGAGCCGCTGCACCGACCAAAGAAAGAACTT
TCAGCTACCAAGAAAGACCGTGTGAATCATTGTCTGACAATATGTGAAAACATAGTGGC
ACAGTCTGTCAGAAATTCTCCAGAATTTCAGAAACTTCTGGGCATCGCTATGGAACTTT
TTCTGCTGTGCAGTGATGACGCAGAGTCAGATGTCAGGATGGTGGCTGACGAATGCCTC
AACAAAGTTATCAAAGCTTTGATGGATTCTAATCTTCCAAGGTTACAGCTCGAGCTCTA
TAAGGAAATTAAAAAGAATGGTGCCCCTCGGAGTTTGCGTGCTGCCCTGTGGAGGTTTG
CTGAGCTGGCTCACCTGGTTCGGCCTCAGAAATGCAGGCCTTACCTGGTGAACCTTCTG
CCGTGCCTGACTCGAACAAGCAAGAGACCCGAAGAATCAGTCCAGGAGACCTTGGCTGC
AGCTGTTCCCAAAATTATGGCTTCTTTTGGCAATTTTGCAAATGACAATGAAATTAAGG
TTTTGTTAAAGGCCTTCATAGCGAACCTGAAGTCAAGCTCCCCCACCATTCGGCGGACA
GCGGCTGGATCAGCAGTGAGCATCTGCCAGCACTCAAGAAGGACACAATATTTCTATAG
TTGGCTACTAAATGTGCTCTTAGGCTTACTCGTTCCTGTCGAGGATGAACACTCCACTC
TGCTGATTCTTGGCGTGCTGCTCACCCTGAGGTATTTGGTGCCCTTGCTGCAGCAGCAG
GTCAAGGACACAAGCCTGAAAGGCAGCTTCGGAGTGACAAGGAAAGAAATGGAAGTCTC
TCCTTCTGCAGAGCAGCTTGTCCAGGTTTATGAACTGACGTTACATCATACACAGCACC
AAGACCACAATGTTGTGACCGGAGCCCTGGAGCTGTTGCAGCAGCTCTTCAGAACGCCT
CCACCCGAGCTTCTGCAAACCCTGACCGCAGTCGGGGGCATTGGGCAGCTCACCGCTGC
TAAGGAGGAGTCTGGTGGCCGAAGCCGTAGTGGGAGTATTGTGGAACTTATAGCTGGAG
GGGGTTCCTCATGCAGCCCTGTCCTTTCAAGAAAACAAAAAGGCAAAGTGCTCTTAGGA
GAAGAAGAAGCCTTGGAGGATGACTCTGAATCGAGATCGGATGTCAGCAGCTCTGCCTT
AACAGCCTCAGTGAAGGATGAGATCAGTGGAGAGCTGGCTGCTTCTTCAGGGGTTTCCA
CTCCAGGGTCAGCAGGTCATGACATCATCACAGAACAGCCACGGTCACAGCACACACTG
CAGGCGGACTCAGTGGATCTGGCCAGCTGTGACTTGACAAGCTCTGCCACTGATGGGGA
TGAGGAGGATATCTTGAGCCACAGCTCCAGCCAGGTCAGCGCCGTCCCATCTGACCCTG
CCATGGACCTGAATGATGGGACCCAGGCCTCGTCGCCCATCAGCGACAGCTCCCAGACC
ACCACCGAAGGGCCTGATTCAGCTGTTACCCCTTCAGACAGTTCTGAAATTGTGTTAGA
CGGTACCGACAACCAGTATTTGGGCCTGCAGATTGGACAGCCCCAGGATGAAGATGAGG
AAGCCACAGGTATTCTTCCTGATGAAGCCTCGGAGGCCTTCAGGAACTCTTCCATGGCC
CTTCAACAGGCACATTTATTGAAAAACATGAGTCACTGCAGGCAGCCTTCTGACAGCAG
TGTTGATAAATTTGTGTTGAGAGATGAAGCTACTGAACCGGGTGATCAAGAAAACAAGC
CTTGCCGCATCAAAGGTGACATTGGACAGTCCACTGATGATGACTCTGCACCTCTTGTC
CATTGTGTCCGCCTTTTATCTGCTTCGTTTTTGCTAACAGGGGGAAAAAATGTGCTGGT
TCCGGACAGGGATGTGAGGGTCAGCGTGAAGGCCCTGGCCCTCAGCTGTGTGGGAGCAG
CTGTGGCCCTCCACCCGGAATCTTTCTTCAGCAAACTCTATAAAGTTCCTCTTGACACC
ACGGAATACCCTGAGGAACAGTATGTCTCAGACATCTTGAACTACATCGATCATGGAGA
CCCACAGGTTCGAGGAGCCACTGCCATTCTCTGTGGGACCCTCATCTGCTCCATCCTCA
GCAGGTCCCGCTTCCACGTGGGAGATTGGATGGGCACCATTAGAACCCTCACAGGAAAT
ACATTTTCTTTGGCGGATTGCATTCCTTTGCTGCGGAAAACACTGAAGGATGAGTCTTC
TGTTACTTGCAAGTTAGCTTGTACAGCTGTGAGGAACTGTGTCATGAGTCTCTGCAGCA
GCAGCTACAGTGAGTTAGGACTGCAGCTGATCATCGATGTGCTGACTCTGAGGAACAGT
TCCTATTGGCTGGTGAGGACAGAGCTTCTGGAAACCCTTGCAGAGATTGACTTCAGGCT
GGTGAGCTTTTTGGAGGCAAAAGCAGAAAACTTACACAGAGGGGCTCATCATTATACAG
GGCTTTTAAAACTGCAAGAACGAGTGCTCAATAATGTTGTCATCCATTTGCTTGGAGAT
GAAGACCCCAGGGTGCGACATGTTGCCGCAGCATCACTAATTAGGCTTGTCCCAAAGCT
GTTTTATAAATGTGACCAAGGACAAGCTGATCCAGTAGTGGCCGTGGCAAGAGATCAAA
GCAGTGTTTACCTGAAACTTCTCATGCATGAAACGCAGCCTCCATCTCATTTCTCCGTC
AGCACAATAACCAGAATATATAGAGGCTATAACCTACTACCAAGCATAACAGACGTCAC
TATGGAAAATAACCTTTCAAGAGTTATTGCAGCAGTTTCTCATGAACTAATCACATCAA
CCACCAGAGCACTCACATTTGGATGCTGTGAAGCTTTGTGTCTTCTTTCCACTGCCTTC
CCAGTTTGCATTTGGAGTTTAGGTTGGCACTGTGGAGTGCCTCCACTGAGTGCCTCAGA
TGAGTCTAGGAAGAGCTGTACCGTTGGGATGGCCACAATGATTCTGACCCTGCTCTCGT
CAGCTTGGTTCCCATTGGATCTCTCAGCCCATCAAGATGCTTTGATTTTGGCCGGAAAC
TTGCTTGCAGCCAGTGCTCCCAAATCTCTGAGAAGTTCATGGGCCTCTGAAGAAGAAGC
CAACCCAGCAGCCACCAAGCAAGAGGAGGTCTGGCCAGCCCTGGGGGACCGGGCCCTGG
TGCCCATGGTGGAGCAGCTCTTCTCTCACCTGCTGAAGGTGATTAACATTTGTGCCCAC
GTCCTGGATGACGTGGCTCCTGGACCCGCAATAAAGGCAGCCTTGCCTTCTCTAACAAA
CCCCCCTTCTCTAAGTCCCATCCGACGAAAGGGGAAGGAGAAAGAACCAGGAGAACAAG
CATCTGTACCGTTGAGTCCCAAGAAAGGCAGTGAGGCCAGTGCAGCTTCTAGACAATCT
GATACCTCAGGTCCTGTTACAACAAGTAAATCCTCATCACTGGGGAGTTTCTATCATCT
TCCTTCATACCTCAAACTGCATGATGTCCTGAAAGCTACACACGCTAACTACAAGGTCA
CGCTGGATCTTCAGAACAGCACGGAAAAGTTTGGAGGGTTTCTCCGCTCAGCCTTGGAT
GTTCTTTCTCAGATACTAGAGCTGGCCACACTGCAGGACATTGGGAAGTGTGTTGAAGA
GATCCTAGGATACCTGAAATCCTGCTTTAGTCGAGAACCAATGATGGCAACTGTTTGTG
TTCAACAATTGTTGAAGACTCTCTTTGGCACAAACTTGGCCTCCCAGTTTGATGGCTTA
TCTTCCAACCCCAGCAAGTCACAAGGCCGAGCACAGCGCCTTGGCTCCTCCAGTGTGAG
GCCAGGCTTGTACCACTACTGCTTCATGGCCCCGTACACCCACTTCACCCAGGCCCTCG
CTGACGCCAGCCTGAGGAACATGGTGCAGGCGGAGCAGGAGAACGACACCTCGGGATGG
TTTGATGTCCTCCAGAAAGTGTCTACCCAGTTGAAGACAAACCTCACGAGTGTCACAAA
GAACCGTGCAGATAAGAATGCTATTCATAATCACATTCGTTTGTTTGAACCTCTTGTTA
TAAAAGCTTTAAAACAGTACACGACTACAACATGTGTGCAGTTACAGAAGCAGGTTTTA
GATTTGCTGGCGCAGCTGGTTCAGTTACGGGTTAATTACTGTCTTCTGGATTCAGATCA
GGTGTTTATTGGCTTTGTATTGAAACAGTTTGAATACATTGAAGTGGGCCAGTTCAGGG
AATCAGAGGCAATCATTCCAAACATCTTTTTCTTCTTGGTATTACTATCTTATGAACGC
TATCATTCAAAACAGATCATTGGAATTCCTAAAATCATTCAGCTCTGTGATGGCATCAT
GGCCAGTGGAAGGAAGGCTGTGACACATGCCATACCGGCTCTGCAGCCCATAGTCCACG
ACCTCTTTGTATTAAGAGGAACAAATAAAGCTGATGCAGGAAAAGAGCTTGAAACCCAA
AAAGAGGTGGTGGTGTCAATGTTACTGAGACTCATCCAGTACCATCAGGTGTTGGAGAT
GTTCATTCTTGTCCTGCAGCAGTGCCACAAGGAGAATGAAGACAAGTGGAAGCGACTGT
CTCGACAGATAGCTGACATCATCCTCCCAATGTTAGCCAAACAGCAGATGCACATTGAC
TCTCATGAAGCCCTTGGAGTGTTAAATACATTATTTGAGATTTTGGCCCCTTCCTCCCT
CCGTCCGGTAGACATGCTTTTACGGAGTATGTTCGTCACTCCAAACACAATGGCGTCCG
TGAGCACTGTTCAACTGTGGATATCGGGAATTCTGGCCATTTTGAGGGTTCTGATTTCC
CAGTCAACTGAAGATATTGTTCTTTCTCGTATTCAGGAGCTCTCCTTCTCTCCGTATTT
AATCTCCTGTACAGTAATTAATAGGTTAAGAGATGGGGACAGTACTTCAACGCTAGAAG
AACACAGTGAAGGGAAACAAATAAAGAATTTGCCAGAAGAAACATTTTCAAGGTTTCTA
TTACAACTGGTTGGTATTCTTTTAGAAGACATTGTTACAAAACAGCTGAAGGTGGAAAT
GAGTGAGCAGCAACATACTTTCTATTGCCAGGAACTAGGCACACTGCTAATGTGTCTGA
TCCACATCTTCAAGTCTGGAATGTTCCGGAGAATCACAGCAGCTGCCACTAGGCTGTTC
CGCAGTGATGGCTGTGGCGGCAGTTTCTACACCCTGGACAGCTTGAACTTGCGGGCTCG
TTCCATGATCACCACCCACCCGGCCCTGGTGCTGCTCTGGTGTCAGATACTGCTGCTTG
TCAACCACACCGACTACCGCTGGTGGGCAGAAGTGCAGCAGACCCCGAAAAGACACAGT
CTGTCCAGCACAAAGTTACTTAGTCCCCAGATGTCTGGAGAAGAGGAGGATTCTGACTT
GGCAGCCAAACTTGGAATGTGCAATAGAGAAATAGTACGAAGAGGGGCTCTCATTCTCT
TCTGTGATTATGTCTGTCAGAACCTCCATGACTCCGAGCACTTAACGTGGCTCATTGTA
AATCACATTCAAGATCTGATCAGCCTTTCCCACGAGCCTCCAGTACAGGACTTCATCAG
TGCCGTTCATCGGAACTCTGCTGCCAGCGGCCTGTTCATCCAGGCAATTCAGTCTCGTT
GTGAAAACCTTTCAACTCCAACCATGCTGAAGAAAACTCTTCAGTGCTTGGAGGGGATC
CATCTCAGCCAGTCGGGAGCTGTGCTCACGCTGTATGTGGACAGGCTTCTGTGCACCCC
TTTCCGTGTGCTGGCTCGCATGGTCGACATCCTTGCTTGTCGCCGGGTAGAAATGCTTC
TGGCTGCAAATTTACAGAGCAGCATGGCCCAGTTGCCAATGGAAGAACTCAACAGAATC
CAGGAATACCTTCAGAGCAGCGGGCTCGCTCAGAGACACCAAAGGCTCTATTCCCTGCT
GGACAGGTTTCGTCTGTCCACCATGCAAGACTCACTTAGTCCCTCTCCTCCAGTCTCTT
CCCACCCGCTGGACGGGGATGGGCACGTGTCACTGGAAACAGTGAGTCCGGACAAAGAC
TGGTACGTTCATCTTGTCAAATCCCAGTGTTGGACCAGGTCAGATTCTGCACTGCTGGA
AGGTGCAGAGCTGGTGAATCGGATTCCTGCTGAAGATATGAATGCCTTCATGATGAACT
CGGAGTTCAACCTAAGCCTGCTAGCTCCATGCTTAAGCCTAGGGATGAGTGAAATTTCT
GGTGGCCAGAAGAGTGCCCTTTTTGAAGCAGCCCGTGAGGTGACTCTGGCCCGTGTGAG
CGGCACCGTGCAGCAGCTCCCTGCTGTCCATCATGTCTTCCAGCCCGAGCTGCCTGCAG
AGCCGGCGGCCTACTGGAGCAAGTTGAATGATCTGTTTGGGGATGCTGCACTGTATCAG
TCCCTGCCCACTCTGGCCCGGGCCCTGGCACAGTACCTGGTGGTGGTCTCCAAACTGCC
CAGTCATTTGCACCTTCCTCCTGAGAAAGAGAAGGACATTGTGAAATTCGTGGTGGCAA
CCCTTGAGGCCCTGTCCTGGCATTTGATCCATGAGCAGATCCCGCTGAGTCTGGATCTC
CAGGCAGGGCTGGACTGCTGCTGCCTGGCCCTGCAGCTGCCTGGCCTCTGGAGCGTGGT
CTCCTCCACAGAGTTTGTGACCCACGCCTGCTCCCTCATCTACTGTGTGCACTTCATCC
TGGAGGCCGTTGCAGTGCAGCCTGGAGAGCAGCTTCTTAGTCCAGAAAGAAGGACAAAT
ACCCCAAAAGCCATCAGCGAGGAGGAGGAGGAAGTAGATCCAAACACACAGAATCCTAA
GTATATCACTGCAGCCTGTGAGATGGTGGCAGAAATGGTGGAGTCTCTGCAGTCGGTGT
TGGCCTTGGGTCATAAAAGGAATAGCGGCGTGCCGGCGTTTCTCACGCCATTGCTCAGG
AACATCATCATCAGCCTGGCCCGCCTGCCCCTTGTCAACAGCTACACACGTGTGCCCCC
ACTGGTGTGGAAGCTTGGATGGTCACCCAAACCGGGAGGGGATTTTGGCACAGCATTCC
CTGAGATCCCCGTGGAGTTCCTCCAGGAAAAGGAAGTCTTTAAGGAGTTCATCTACCGC
ATCAACACACTAGGCTGGACCAGTCGTACTCAGTTTGAAGAAACTTGGGCCACCCTCCT
TGGTGTCCTGGTGACGCAGCCCCTCGTGATGGAGCAGGAGGAGAGCCCACCAGAAGAAG
ACACAGAGAGGACCCAGATCAACGTCCTGGCCGTGCAGGCCATCACCTCACTGGTGCTC
AGTGCAATGACTGTGCCTGTGGCCGGCAACCCAGCTGTAAGCTGCTTGGAGCAGCAGCC
CCGGAACAAGCCTCTGAAAGCTCTCGACACCAGGTTTGGGAGGAAGCTGAGCATTATCA
GAGGGATTGTGGAGCAAGAGATTCAAGCAATGGTTTCAAAGAGAGAGAATATTGCCACC
CATCATTTATATCAGGCATGGGATCCTGTCCCTTCTCTGTCTCCGGCTACTACAGGTGC
CCTCATCAGCCACGAGAAGCTGCTGCTACAGATCAACCCCGAGCGGGAGCTGGGGAGCA
TGAGCTACAAACTCGGCCAGGTGTCCATACACTCCGTGTGGCTGGGGAACAGCATCACA
CCCCTGAGGGAGGAGGAATGGGACGAGGAAGAGGAGGAGGAGGCCGACGCCCCTGCACC
TTCGTCACCACCCACTTCTCCAGTCAACTCCAGGAAACACCGGGCTGGAGTTGACATCC
ACTCCTGTTCGCAGTTTTTGCTTGAGTTGTACAGCCGCTGGATCCTGCCGTCCAGCTCA
GCCAGGAGGACCCCGGCCATCCTGATCAGTGAGGTGGTCAGATCCCTTCTAGTGGTCTC
AGACTTGTTCACCGAGCGCAACCAGTTTGAGCTGATGTATGTGACGCTGACAGAACTGC
GAAGGGTGCACCCTTCAGAAGACGAGATCCTCGCTCAGTACCTGGTGCCTGCCACCTGC
AAGGCAGCTGCCGTCCTTGGGATGGACAAGGCCGTGGCGGAGCCTGTCAGCCGCCTGCT
GGAGAGCACGCTCAGGAGCAGCCACCTGCCCAGCAGGGTTGGAGCCCTGCACGGCGTCC
TCTATGTGCTGGAGTGCGACCTGCTGGACGACACTGCCAAGCAGCTCATCCCGGTCATC
AGCGACTATCTCCTCTCCAACCTGAAAGGGATCGCCCACTGCGTGAACATTCACAGCCA
GCAGCACGTACTGGTCATGTGTGCCACTGCGTTTTACCTCATTGAGAACTATCCTCTGG
ACGTAGGGCCGGAATTTTCAGCATCAATAATACAGATGTGTGGGGTGATGCTGTCTGGA
AGTGAGGAGTCCACCCCCTCCATCATTTACCACTGTGCCCTCAGAGGCCTGGAGCGCCT
CCTGCTCTCTGAGCAGCTCTCCCGCCTGGATGCAGAATCGCTGGTCAAGCTGAGTGTGG
ACAGAGTGAACGTGCACAGCCCGCACCGGGCCATGGCGGCTCTGGGCCTGATGCTCACC
TGCATGTACACAGGAAAGGAGAAAGTCAGTCCGGGTAGAACTTCAGACCCTAATCCTGC
AGCCCCCGACAGCGAGTCAGTGATTGTTGCTATGGAGCGGGTATCTGTTCTTTTTGATA
GGATCAGGAAAGGCTTTCCTTGTGAAGCCAGAGTGGTGGCCAGGATCCTGCCCCAGTTT
CTAGACGACTTCTTCCCACCCCAGGACATCATGAACAAAGTCATCGGAGAGTTTCTGTC
CAACCAGCAGCCATACCCCCAGTTCATGGCCACCGTGGTGTATAAGGTGTTTCAGACTC
TGCACAGCACCGGGCAGTCGTCCATGGTCCGGGACTGGGTCATGCTGTCCCTCTCCAAC
TTCACGCAGAGGGCCCCGGTCGCCATGGCCACGTGGAGCCTCTCCTGCTTCTTTGTCAG
CGCGTCCACCAGCCCGTGGGTCGCGGCGATCCTCCCACATGTCATCAGCAGGATGGGCA
AGCTGGAGCAGGTGGACGTGAACCTTTTCTGCCTGGTCGCCACAGACTTCTACAGACAC
CAGATAGAGGAGGAGCTCGACCGCAGGGCCTTCCAGTCTGTGCTTGAGGTGGTTGCAGC
CCCAGGAAGCCCATATCACCGGCTGCTGACTTGTTTACGAAATGTCCACAAGGTCACCA
CCTGCGCGGCCGC
Cloning Information
The construct is engineered with a "PmeI" on the 5' and NotI on the 3' prime. Cloning site: fused EcoRV/PmeI at 5' and Not I at 3', RV and PmeI were deleted.
Phenotypic Data
Remarks
DNA corresponding to the full length human sequence of the Huntingtin (Htt) gene containing 23 polyglutamine repeats translated from a random codon. rdm: x/CAG/CAA/x
External Links
Gene Cards
HD
HD (verified)
Gene Ontology
GO:0003714 transcription corepressor activity
GO:0005215 transporter activity
GO:0005515 protein binding
GO:0005625 soluble fraction
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0006915 apoptosis
GO:0006917 induction of apoptosis
GO:0007610 behavior
GO:0008017 microtubule binding
GO:0009405 pathogenesis
GO:0009887 organogenesis
NCBI Gene
Gene ID:3064
NCBI GTR
143100 HUNTINGTON DISEASE; HD
OMIM
143100 HUNTINGTON DISEASE; HD
Website
Invitrogen website
Culture Protocols
DNA corresponding to the full length human sequence of the Huntingtin (Htt) gene containing 23 polyglutamine repeats translated from a random codon. rdm: x/CAG/CAA/x
Remarks
DNA corresponding to the full length human sequence of the Huntingtin (Htt) gene containing 23 polyglutamine repeats translated from a random codon. rdm: x/CAG/CAA/x
PDF
Vector Map
Plasmid Collection Screening and Propagation
Supplement
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Pricing
Commercial:
$50.00
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Academic &
Non-profit:
$50.00
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