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CH01793
Plasmid
Description:
HTT-Q22-PCDNA3.1, 1-3144, RDM, NTERMHIS6-FLAG, HUMAN
Affected:
No Data
Sex:
No Data
Age:
No Data
Sample Description
Overview
Characterizations
Phenotypic Data
Culture Protocols
Overview
Repository
HD Community Biorepository
Quantity
20 µg
Quantitation Method
Please see our
FAQ
Certain Third Party Licensing Requirements
Invitrogen License for Cat# V790.20
Family History
N
Species
Homo
sapiens
Common Name
Human
Remarks
DNA corresponding to codon optimized N-terminally tagged 6xHISFLAG full length Huntingtin (Htt) containing 22 polyglutamine repeats from a random CAG codon in pcDNA3.1 vector
Characterizations
Remarks
DNA corresponding to codon optimized N-terminally tagged 6xHISFLAG full length Huntingtin (Htt) containing 22 polyglutamine repeats from a random CAG codon in pcDNA3.1 vector
Construct Name
Insert Length
9498
Vector
pcDNA3.1
Concentration
20ug
Sequence
GAATTCCCGCCGCCACCATGCACCACCACCACCACCATGATTATAAGGATGATGATGAT
AAGGCTACTCTGGAAAAACTGATGAAGGCATTTGAGTCTCTGAAGAGCTTCCAGCAACA
ACAGCAACAACAGCAACAGCAACAGCAGCAACAACAGCAGCAACAGCAACAACAACAGC
CACCTCCTCCTCCACCTCCACCTCCACCCCCTCAGCTGCCCCAGCCACCACCTCAGGCA
CAGCCTCTGCTGCCACAGCCCCAGCCACCCCCTCCTCCTCCTCCACCACCTCCAGGACC
AGCAGTGGCCGAGGAGCCTCTGCACAGACCAAAGAAGGAGCTGAGCGCCACAAAGAAGG
ATAGGGTGAACCACTGTCTGACCATCTGCGAGAATATCGTGGCCCAGTCTGTGAGGAAC
AGCCCTGAGTTTCAGAAGCTGCTGGGCATCGCCATGGAGCTGTTCCTGCTGTGCTCTGA
CGATGCCGAGAGCGATGTGCGGATGGTGGCCGACGAGTGTCTGAACAAAGTGATCAAGG
CCCTGATGGACAGCAACCTGCCCAGACTGCAGCTGGAGCTGTACAAGGAGATCAAGAAG
AATGGAGCACCTAGGTCCCTGAGGGCCGCCCTGTGGCGCTTCGCCGAGCTGGCCCACCT
GGTGCGGCCTCAGAAGTGTAGACCATATCTGGTGAACCTGCTGCCCTGCCTGACACGGA
CCTCCAAGAGACCTGAGGAGTCTGTGCAGGAGACCCTGGCAGCAGCAGTGCCTAAGATC
ATGGCCAGCTTTGGCAATTTCGCCAACGACAATGAGATCAAGGTGCTGCTGAAGGCCTT
TATCGCCAACCTGAAGAGCTCCTCTCCAACCATCAGGAGAACAGCAGCAGGAAGCGCCG
TGTCCATCTGCCAGCACAGCAGGCGCACCCAGTACTTCTATTCCTGGCTGCTGAATGTG
CTGCTGGGACTGCTGGTGCCAGTGGAGGATGAGCACTCCACACTGCTGATCCTGGGCGT
GCTGCTGACCCTGCGGTACCTGGTGCCTCTGCTGCAGCAGCAGGTGAAGGACACATCCC
TGAAGGGCTCTTTTGGCGTGACCAGAAAGGAGATGGAGGTGTCCCCCTCTGCCGAGCAG
CTGGTGCAGGTGTATGAGCTGACCCTGCACCACACACAGCACCAGGACCACAACGTGGT
GACAGGCGCCCTGGAGCTGCTGCAGCAGCTGTTCCGGACCCCACCTCCAGAGCTGCTGC
AGACACTGACCGCAGTGGGAGGAATCGGACAGCTGACCGCAGCAAAGGAGGAGAGCGGA
GGCCGGAGCAGATCCGGATCTATCGTGGAGCTGATCGCAGGAGGAGGAAGCTCCTGCAG
CCCCGTGCTGTCCAGGAAGCAGAAGGGCAAGGTGCTGCTGGGAGAGGAGGAGGCCCTGG
AGGACGATAGCGAGTCCCGCTCTGACGTGAGCAGCTCCGCCCTGACAGCCAGCGTGAAG
GACGAGATCTCCGGAGAGCTGGCAGCATCTAGCGGCGTGTCTACCCCTGGAAGCGCCGG
CCACGACATCATCACAGAGCAGCCAAGAAGCCAGCACACCCTGCAGGCCGATTCCGTGG
ACCTGGCCTCTTGTGATCTGACCTCCTCTGCCACAGATGGCGACGAGGAGGACATCCTG
AGCCACAGCTCCTCTCAGGTGTCCGCCGTGCCTTCTGATCCAGCCATGGATCTGAATGA
CGGAACCCAGGCCAGCTCCCCAATCTCCGACTCTAGCCAGACCACAACCGAGGGCCCTG
ATAGCGCCGTGACACCATCCGACTCCTCTGAGATCGTGCTGGATGGCACCGACAACCAG
TACCTGGGACTGCAGATCGGACAGCCACAGGATGAGGACGAGGAGGCAACCGGCATCCT
GCCTGATGAGGCCAGCGAGGCCTTTCGGAATAGCTCCATGGCACTGCAGCAGGCACACC
TGCTGAAGAACATGTCTCACTGCAGACAGCCTAGCGATTCTAGCGTGGACAAGTTCGTG
CTGAGGGATGAGGCAACAGAGCCAGGCGACCAGGAGAATAAGCCTTGCCGGATCAAGGG
CGACATCGGCCAGTCTACCGACGATGACAGCGCCCCACTGGTGCACTGCGTGCGGCTGC
TGAGCGCCTCCTTCCTGCTGACCGGAGGCAAGAACGTGCTGGTGCCCGATCGGGACGTG
AGAGTGTCTGTGAAGGCCCTGGCCCTGAGCTGCGTGGGAGCAGCCGTGGCCCTGCACCC
TGAGTCTTTCTTTAGCAAGCTGTACAAGGTGCCACTGGATACAACCGAGTACCCCGAGG
AGCAGTATGTGAGCGACATCCTGAATTATATCGATCACGGCGACCCACAGGTGCGGGGA
GCAACCGCCATCCTGTGCGGCACACTGATCTGCTCTATCCTGTCTAGGAGCCGCTTTCA
CGTGGGCGACTGGATGGGCACAATCAGGACACTGACCGGCAACACCTTCTCCCTGGCCG
ATTGCATCCCCCTGCTGCGCAAGACACTGAAGGACGAGTCCTCTGTGACCTGTAAGCTG
GCCTGCACAGCCGTGAGAAATTGCGTGATGTCCCTGTGCAGCTCCTCTTACTCTGAGCT
GGGCCTGCAGCTGATCATCGATGTGCTGACCCTGAGAAACAGCTCCTATTGGCTGGTGA
GGACCGAGCTGCTGGAGACACTGGCCGAGATCGACTTTAGACTGGTGAGCTTCCTGGAG
GCCAAGGCCGAGAATCTGCACAGGGGAGCACACCACTACACCGGACTGCTGAAGCTGCA
GGAGCGCGTGCTGAACAACGTGGTCATCCACCTGCTGGGCGATGAGGACCCACGGGTGA
GACACGTGGCAGCAGCCAGCCTGATCAGGCTGGTGCCTAAGCTGTTTTACAAGTGTGAT
CAGGGACAGGCAGACCCAGTGGTGGCAGTGGCAAGGGATCAGTCTAGCGTGTATCTGAA
GCTGCTGATGCACGAGACCCAGCCCCCTTCCCACTTCTCCGTGTCTACAATCACCAGGA
TCTACCGCGGCTATAACCTGCTGCCCTCCATCACAGACGTGACCATGGAGAACAATCTG
TCTAGAGTGATCGCCGCCGTGTCCCACGAGCTGATCACCTCTACAACCCGCGCCCTGAC
ATTTGGCTGCTGTGAGGCCCTGTGCCTGCTGTCCACAGCCTTCCCCGTGTGCATCTGGT
CTCTGGGATGGCACTGCGGAGTGCCACCACTGAGCGCCTCCGATGAGTCCAGGAAGTCT
TGTACCGTGGGCATGGCCACCATGATCCTGACACTGCTGTCCTCTGCCTGGTTTCCACT
GGATCTGAGCGCCCACCAGGACGCACTGATCCTGGCAGGAAATCTGCTGGCAGCAAGCG
CCCCAAAGTCCCTGCGGAGCTCCTGGGCATCCGAGGAGGAGGCAAACCCAGCAGCAACC
AAGCAGGAGGAAGTGTGGCCAGCCCTGGGCGACAGAGCCCTGGTGCCAATGGTGGAGCA
GCTGTTCAGCCACCTGCTGAAAGTGATCAACATCTGCGCACACGTGCTGGATGACGTGG
CACCTGGACCAGCCATCAAGGCCGCCCTGCCTTCCCTGACAAACCCTCCATCTCTGAGC
CCAATCCGGAGAAAGGGCAAGGAGAAGGAGCCAGGAGAGCAGGCCTCTGTGCCACTGAG
CCCTAAGAAGGGAAGCGAGGCCTCCGCCGCATCTAGGCAGAGCGACACCTCCGGCCCAG
TGACAACCAGCAAGTCTAGCTCCCTGGGCAGCTTTTACCACCTGCCCTCCTATCTGAAG
CTGCACGATGTGCTGAAGGCCACCCACGCCAATTACAAGGTGACACTGGACCTGCAGAA
CTCCACCGAGAAGTTTGGCGGCTTCCTGAGGTCTGCCCTGGATGTGCTGAGCCAGATCC
TGGAGCTGGCCACACTGCAGGACATCGGCAAGTGCGTGGAGGAGATCCTGGGCTATCTG
AAGTCTTGCTTCAGCCGCGAGCCAATGATGGCCACCGTGTGCGTGCAGCAGCTGCTGAA
GACCCTGTTTGGCACAAATCTGGCCAGCCAGTTCGATGGCCTGTCTAGCAACCCATCCA
AGTCTCAGGGAAGGGCACAGAGACTGGGATCCTCTAGCGTGAGGCCTGGCCTGTACCAC
TATTGCTTTATGGCCCCATACACACACTTCACCCAGGCCCTGGCAGACGCCTCCCTGCG
CAATATGGTGCAGGCCGAGCAGGAGAACGATACATCTGGCTGGTTTGACGTGCTGCAGA
AGGTGAGCACCCAGCTGAAGACAAACCTGACCTCCGTGACAAAGAATAGAGCCGATAAG
AACGCCATCCACAATCACATCAGGCTGTTCGAGCCCCTGGTCATCAAGGCCCTGAAGCA
GTATACAACCACAACCTGCGTGCAGCTGCAGAAGCAGGTGCTGGACCTGCTGGCCCAGC
TGGTGCAGCTGAGGGTGAATTACTGTCTGCTGGATTCCGACCAGGTGTTTATCGGCTTC
GTGCTGAAGCAGTTTGAGTATATCGAAGTGGGCCAGTTCCGGGAGTCTGAGGCCATCAT
CCCTAACATCTTCTTTTTCCTGGTGCTGCTGTCCTACGAGAGATATCACTCTAAGCAGA
TCATCGGCATCCCAAAGATCATCCAGCTGTGCGACGGCATCATGGCAAGCGGAAGGAAG
GCAGTGACCCACGCAATCCCCGCCCTGCAGCCTATCGTGCACGATCTGTTTGTGCTGCG
CGGCACAAATAAGGCAGACGCAGGCAAGGAGCTGGAGACCCAGAAGGAGGTGGTGGTGT
CCATGCTGCTGCGCCTGATCCAGTACCACCAGGTGCTGGAGATGTTCATCCTGGTGCTG
CAGCAGTGTCACAAGGAGAACGAGGATAAGTGGAAGAGGCTGTCCCGCCAGATCGCCGA
CATCATCCTGCCCATGCTGGCCAAGCAGCAGATGCACATCGATTCTCACGAGGCCCTGG
GCGTGCTGAATACCCTGTTTGAGATCCTGGCACCATCCTCTCTGAGGCCTGTGGACATG
CTGCTGAGATCTATGTTCGTGACCCCTAACACAATGGCCAGCGTGTCCACAGTGCAGCT
GTGGATCAGCGGCATCCTGGCCATCCTGCGGGTGCTGATCTCTCAGAGCACCGAGGATA
TCGTGCTGAGCAGAATCCAGGAGCTGTCCTTTTCTCCATACCTGATCTCCTGCACCGTG
ATCAATAGGCTGCGCGATGGCGACAGCACATCCACCCTGGAGGAGCACAGCGAGGGCAA
GCAGATCAAGAACCTGCCCGAGGAGACATTTTCCCGGTTCCTGCTGCAGCTGGTGGGCA
TCCTGCTGGAGGACATCGTGACCAAGCAGCTGAAGGTGGAGATGAGCGAGCAGCAGCAC
ACATTCTATTGTCAGGAGCTGGGCACCCTGCTGATGTGCCTGATCCACATCTTTAAGTC
CGGAATGTTCAGGAGGATCACCGCAGCAGCAACAAGGCTGTTTAGAAGCGATGGCTGCG
GCGGCTCCTTCTACACACTGGACTCTCTGAATCTGCGGGCCAGAAGCATGATCACAACC
CACCCCGCCCTGGTGCTGCTGTGGTGTCAGATCCTGCTGCTGGTGAACCACACCGACTA
TAGGTGGTGGGCAGAGGTGCAGCAGACACCAAAGAGGCACAGCCTGAGCTCCACCAAGC
TGCTGTCCCCTCAGATGTCTGGAGAGGAGGAGGATTCCGACCTGGCAGCAAAGCTGGGC
ATGTGCAACAGAGAGATCGTGCGGAGAGGCGCCCTGATCCTGTTCTGTGATTACGTGTG
CCAGAATCTGCACGACAGCGAGCACCTGACCTGGCTGATCGTGAACCACATCCAGGATC
TGATCTCTCTGAGCCACGAGCCCCCTGTGCAGGACTTTATCTCCGCCGTGCACCGGAAC
AGCGCCGCATCTGGCCTGTTCATCCAGGCCATCCAGAGCAGATGCGAGAACCTGTCCAC
ACCCACCATGCTGAAGAAGACCCTGCAGTGTCTGGAGGGCATCCACCTGTCCCAGTCTG
GCGCCGTGCTGACACTGTATGTGGATAGGCTGCTGTGCACCCCCTTTAGGGTGCTGGCC
CGCATGGTGGACATCCTGGCCTGTAGGCGCGTGGAGATGCTGCTGGCCGCCAATCTGCA
GTCTAGCATGGCCCAGCTGCCTATGGAGGAGCTGAACCGCATCCAGGAGTACCTGCAGT
CCTCTGGACTGGCACAGAGGCACCAGCGCCTGTATTCTCTGCTGGATAGGTTCCGCCTG
AGCACCATGCAGGACAGCCTGTCCCCATCTCCACCCGTGAGCTCCCACCCACTGGATGG
CGACGGACACGTGTCCCTGGAGACAGTGTCTCCCGATAAGGACTGGTACGTGCACCTGG
TGAAGAGCCAGTGCTGGACCAGGAGCGATTCCGCCCTGCTGGAGGGAGCAGAGCTGGTG
AATCGCATCCCTGCCGAGGACATGAACGCCTTTATGATGAACAGCGAGTTCAATCTGTC
CCTGCTGGCCCCATGTCTGTCTCTGGGCATGAGCGAGATCTCCGGAGGACAGAAGAGCG
CCCTGTTTGAGGCCGCCAGGGAGGTGACACTGGCCCGCGTGTCCGGAACCGTGCAGCAG
CTGCCAGCAGTGCACCACGTGTTTCAGCCAGAGCTGCCAGCAGAGCCTGCAGCATACTG
GTCTAAGCTGAACGATCTGTTCGGCGACGCCGCCCTGTACCAGAGCCTGCCTACACTGG
CAAGAGCCCTGGCACAGTATCTGGTGGTGGTGAGCAAGCTGCCATCCCACCTGCACCTG
CCTCCAGAGAAGGAGAAGGATATCGTGAAGTTCGTGGTGGCCACCCTGGAGGCCCTGAG
CTGGCACCTGATCCACGAGCAGATCCCACTGTCCCTGGATCTGCAGGCAGGACTGGACT
GCTGTTGCCTGGCCCTGCAGCTGCCAGGACTGTGGTCTGTGGTGTCTAGCACCGAGTTT
GTGACACACGCCTGTAGCCTGATCTACTGCGTGCACTTCATCCTGGAGGCAGTGGCAGT
GCAGCCTGGAGAGCAGCTGCTGTCCCCAGAGCGGAGAACAAATACCCCCAAGGCCATCT
CTGAGGAGGAGGAGGAGGTGGACCCAAACACACAGAATCCCAAGTATATCACCGCAGCA
TGCGAGATGGTGGCAGAGATGGTCGAGTCTCTGCAGAGCGTGCTGGCCCTGGGACACAA
GCGGAACAGCGGAGTGCCTGCCTTCCTGACACCACTGCTGCGCAATATCATCATCTCTC
TGGCCCGGCTGCCCCTGGTGAACAGCTACACCAGAGTGCCCCCTCTGGTGTGGAAGCTG
GGATGGTCCCCAAAGCCTGGAGGCGATTTTGGCACCGCCTTCCCAGAGATCCCCGTGGA
GTTTCTGCAGGAGAAGGAGGTGTTTAAGGAGTTCATCTATCGCATCAATACACTGGGCT
GGACCAGCCGGACACAGTTCGAGGAGACCTGGGCCACACTGCTGGGCGTGCTGGTGACC
CAGCCTCTGGTCATGGAGCAGGAGGAGTCCCCACCAGAGGAGGACACCGAGAGGACACA
GATCAACGTGCTGGCCGTGCAGGCCATCACATCTCTGGTGCTGAGCGCCATGACCGTGC
CAGTGGCAGGAAATCCTGCCGTGAGCTGCCTGGAGCAGCAGCCAAGAAACAAGCCCCTG
AAGGCCCTGGATACACGGTTCGGCAGAAAGCTGTCCATCATCCGGGGCATCGTGGAGCA
GGAGATCCAGGCCATGGTGTCTAAGAGAGAGAACATCGCCACCCACCACCTGTACCAGG
CCTGGGACCCAGTGCCCTCTCTGAGCCCAGCAACAACCGGCGCCCTGATCAGCCACGAG
AAGCTGCTGCTGCAGATCAATCCTGAGAGGGAGCTGGGCTCCATGTCTTATAAGCTGGG
CCAGGTGTCCATCCACAGCGTGTGGCTGGGCAACTCCATCACACCCCTGCGCGAGGAGG
AGTGGGATGAAGAGGAGGAGGAGGAGGCAGACGCACCTGCACCATCCTCTCCTCCAACC
TCCCCTGTGAACAGCAGGAAGCACCGCGCCGGCGTGGATATCCACAGCTGTTCCCAGTT
CCTGCTGGAGCTGTACAGCAGATGGATCCTGCCTAGCTCCTCTGCCAGGCGCACACCAG
CCATCCTGATCTCTGAGGTGGTGCGCAGCCTGCTGGTGGTGTCCGACCTGTTTACCGAG
CGGAACCAGTTCGAGCTGATGTACGTGACACTGACCGAGCTGCGGAGAGTGCACCCAAG
CGAGGATGAGATCCTGGCCCAGTATCTGGTGCCAGCAACATGCAAGGCAGCAGCCGTGC
TGGGAATGGACAAGGCAGTGGCAGAGCCCGTGAGCAGGCTGCTGGAGAGCACCCTGCGC
AGCTCCCACCTGCCATCCAGGGTGGGCGCCCTGCACGGCGTGCTGTACGTGCTGGAGTG
TGATCTGCTGGATGACACCGCCAAGCAGCTGATCCCCGTGATCTCCGACTATCTGCTGT
CTAATCTGAAGGGCATCGCCCACTGCGTGAACATCCACAGCCAGCAGCACGTGCTGGTC
ATGTGCGCCACAGCCTTTTACCTGATCGAGAACTATCCACTGGACGTGGGCCCCGAGTT
CTCTGCCAGCATCATCCAGATGTGCGGCGTGATGCTGTCCGGCTCTGAGGAGAGCACCC
CATCCATCATCTACCACTGTGCACTGAGGGGACTGGAGAGACTGCTGCTGAGCGAGCAG
CTGTCCAGGCTGGATGCCGAGAGCCTGGTGAAGCTGTCCGTGGACAGAGTGAATGTGCA
CAGCCCACACAGGGCAATGGCCGCCCTGGGACTGATGCTGACCTGCATGTATACAGGCA
AGGAGAAGGTGTCTCCAGGAAGGACCAGCGATCCAAACCCTGCAGCACCTGACAGCGAG
TCCGTGATCGTGGCCATGGAGCGCGTGTCCGTGCTGTTTGATCGGATCAGAAAGGGCTT
CCCATGTGAGGCAAGGGTGGTGGCACGCATCCTGCCACAGTTTCTGGATGACTTTTTCC
CCCCTCAGGACATCATGAACAAAGTGATCGGCGAGTTCCTGTCTAACCAGCAGCCATAC
CCCCAGTTTATGGCCACCGTGGTGTATAAGGTGTTCCAGACCCTGCACAGCACAGGCCA
GTCTAGCATGGTGCGCGATTGGGTCATGCTGTCTCTGAGCAACTTCACCCAGAGGGCAC
CTGTGGCAATGGCAACCTGGTCCCTGTCTTGCTTTTTCGTGAGCGCCTCCACATCTCCT
TGGGTGGCCGCCATCCTGCCACACGTGATCAGCCGGATGGGCAAGCTGGAGCAGGTGGA
TGTGAACCTGTTTTGTCTGGTGGCCACCGACTTCTACAGACACCAGATCGAGGAGGAGC
TGGACAGGAGGGCATTCCAGTCCGTGCTGGAGGTGGTGGCCGCCCCAGGCTCCCCATAC
CATAGGCTGCTGACCTGTCTGAGGAATGTGCATAAAGTGACTACCTGTTGATAAGCTG
Cloning Information
DNA corresponding to codon optimized N-terminally tagged 6xHIS-FLAG full length Huntingtin (Htt) containing 22 polyglutamine repeats from a random CAG codon in pcDNA3.1 vector.
Phenotypic Data
Remarks
DNA corresponding to codon optimized N-terminally tagged 6xHISFLAG full length Huntingtin (Htt) containing 22 polyglutamine repeats from a random CAG codon in pcDNA3.1 vector
Culture Protocols
DNA corresponding to codon optimized N-terminally tagged 6xHISFLAG full length Huntingtin (Htt) containing 22 polyglutamine repeats from a random CAG codon in pcDNA3.1 vector
Remarks
DNA corresponding to codon optimized N-terminally tagged 6xHISFLAG full length Huntingtin (Htt) containing 22 polyglutamine repeats from a random CAG codon in pcDNA3.1 vector
PDF
Vector Map
Plasmid Collection Screening and Propagation
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