AG07374
Fibroblast from Skin, Arm
Description:
ALZHEIMER DISEASE; AD
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Alzheimer's Disease
Alzheimer Disease With No Family History |
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Biopsy Source
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Arm
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Arm
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
5.62 |
| Passage Frozen |
14 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The donor had a history of progressive dementia including loss of memory, confusion and disorientation, eventually requiring institutional placement. The skin biopsy was taken ante-mortem from the upper left arm. The culture was initiated on 2/28/84 using explants of minced skin tissue. The cell morphology is fibroblast-like. The culture is a mosaic with karyotype: 45,X/46,XY; unbalanced; 52%/48%. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Ramamoorthy M, Sykora P, Scheibye-Knudsen M, Dunn C, Kasmer C, Zhang Y, Becker KG, Croteau DL, Bohr VA, Sporadic Alzheimer disease fibroblasts display an oxidative stress phenotype Free radical biology & medicine53:1371-80 2012 |
| PubMed ID: 22885031 |
| Cumulative PDL at Freeze |
14.7 |
| Passage Frozen |
14 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
3% |
| Medium |
Eagles Minimum Essential Medium with Earle's salts:Dulbecco's modified MEM with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Supplement |
- |
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