AG09107
LCL from B-Lymphocyte
Description:
ALZHEIMER DISEASE; AD
Repository
|
NIA Aging Cell Culture Repository
|
Subcollection |
Alzheimer's Disease |
Biopsy Source
|
Peripheral vein
|
Cell Type
|
B-Lymphocyte
|
Tissue Type
|
Blood
|
Transformant
|
Epstein-Barr Virus
|
Sample Source
|
LCL from B-Lymphocyte
|
Race
|
White
|
Ethnicity
|
AMERICAN
|
Family Member
|
7
|
Relation to Proband
|
nephew
|
Confirmation
|
Clinical summary/Case history
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
Remarks |
The donor is 50% at risk for Alzheimer's disease. Donor's mother, two uncles and grandmother were affected. The culture was initiated by transformation of lymphocytes with Epstein Barr virus. The cells grow in suspension and their morphology is spherical. Donor's affected maternal uncle is AG00364E Fibroblast. Donor is brother of AG09108 and AG09109. |
Split Ratio |
1:3 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not Inactivated |
Substrate |
None specified |
Subcultivation Method |
dilution - add fresh medium |
Supplement |
- |
|
|