AG10760
LCL from B-Lymphocyte
Description:
ALZHEIMER DISEASE; AD
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Alzheimer's Disease |
|
Biopsy Source
|
Peripheral vein
|
|
Cell Type
|
B-Lymphocyte
|
|
Tissue Type
|
Blood
|
|
Transformant
|
Epstein-Barr Virus
|
|
Sample Source
|
LCL from B-Lymphocyte
|
|
Race
|
White
|
|
Ethnicity
|
ITALIAN
|
|
Family Member
|
9
|
|
Relation to Proband
|
in-law
|
|
Confirmation
|
Clinical summary/Case history
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Remarks |
The donor is an unaffected spouse of an Alzheimer's disease affected family member. The culture was initiated on 12/15/89 by transformation of lymphocytes with Epstein Barr virus. The cells grow in suspension and their morphology is spherical. Donor's children are AG10757, AG10758, AG10759, and AG10761A. Deceased affected spouse was maternal uncle of proband. |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
|
|