AG10774
Endothelial from Vein, iliac
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Vein, iliac
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Cell Type
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Endothelial
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Tissue Type
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Vein
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Transformant
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Untransformed
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Sample Source
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Endothelial from Vein, iliac
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Senescence |
27 |
| PDL at Freeze |
18 |
| Passage Frozen |
6 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Line HIVE-87. The iliac vein was removed from a braindead, heartbeating cadaver, organ donor on 6/09/87. The donor died of a closed head trauma and had no known diseases. The culture was initiated on 6/09/87 using cells released from the lumen of the vessel by collagenase digestion. Cell morphology is polygonal. This culture tested positive for the endothelial cell-specific von Willebrand factor and negative for alphasmooth muscle actin (immunofluorescence assays). The culture was grown on gelatin-coated flasks and the medium was supplemented with 0.02 mg/ml ECGS and 0.05 mg/ml heparin. Cells were frozen in growth medium containing 7.5% polyvinylpyrrolidinone and 5% DMSO. |
| Admasu TD, Kim K, Rae M, Avelar R, Gonciarz RL, Rebbaa A, Pedro de Magalhães J, Renslo AR, Stolzing A, Sharma A, Selective ablation of primary and paracrine senescent cells by targeting iron dyshomeostasis Cell reports42:112058 2022 |
| PubMed ID: 36753419 |
| PDL at Senescence |
27 |
| Cumulative PDL at Freeze |
18 |
| Passage Frozen |
6 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Medium 199 in Earl BSS with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Heat Inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Endothelial Cell Growth Supplement 0.02 mg/ml |
| Supplement |
Heparin 0.05 mg/ml |
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