AG11682
LCL from B-Lymphocyte
Description:
AGED SIB PAIRS COLLECTION
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Aged Sib Pairs
Apparently Healthy Collection |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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White
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Ethnicity
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GERMAN
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Family Member
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3
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Relation to Proband
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brother
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Donor has hypertension and is on hydrochlorothiazide. The culture was initiated on 10/22/91 by transformation of lymphocytes with Epstein Barr virus. The culture grows in suspension and the cell morphology is spherical. Lymphoblast cultures from the siblings of this donor are AG11680, AG11681B, and AG11684. |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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