Description:
CHROMOSOME INSERTION
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Chromosome Abnormalities |
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Cell Type
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Fibroblast
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Transformant
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Untransformed
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Race
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Black/African American
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Family Member
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2
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Relation to Proband
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mother
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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46,XX,ins(5;6)(5pter>5q33::6q15>6q25:: 5q33>5qter;6pter>6q15::6q25>6qter)
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
9 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Cytogenetics |
Chromosome 5: INSERTION Breakpoint 5q33 ins(5;6)5q33 |
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Chromosome 6: INSERTION Breakpoint 6q15 ins(5;6)6q15 |
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Chromosome 6: INSERTION Breakpoint 6q25 ins(5;6)6q25 |
| Remarks |
Mother and half-brother have same balanced insertion; normal phenotype |
| Chen H, Tyrkus M, Cohen F, Woolley PV Jr, Mayeda K, Bhogaonker A, Espirtu CE, Simpson W, Familial partial trisomy 6q syndromes resulting from inherited ins (5;6) (q33;q15q27). Clin Genet9:631-7 1976 |
| PubMed ID: 1277576 |
| Passage Frozen |
9 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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