Description:
TRANSLOCATED CHROMOSOME
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Chromosome Abnormalities |
|
Cell Type
|
Fibroblast
|
|
Transformant
|
Untransformed
|
|
Race
|
White
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Karyotypic analysis after cell line submission to CCR
|
|
ISCN
|
46,X,t(X;15)(Xqter>Xp11::15q13>15qter; 15pter>15q13::Xp11>Xpter),t(1;21) (1pter>1q12::21q22>21qter;21pter>21q22::1q12>1qter)pat
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| Passage Frozen |
3 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
| |
| Cytogenetics |
Chromosome 1: TRANSLOCATION Breakpoint 1q12 t(1;21)1q12 |
|
Chromosome 15: TRANSLOCATION Breakpoint 15q13 t(X;15)15q13 |
|
Chromosome 21: TRANSLOCATION Breakpoint 21q22 t(1;21)21q22 |
|
Chromosome X: TRANSLOCATION Breakpoint Xp11 t(X;15)Xp11 |
| Remarks |
Menstrual dysfunction; t(X;15) present in 100% of cells at passage 6; it was not found in peripheral blood leukocytes |
| Lafreniere RG, Brown CJ, Powers VE, Carrel L, Davies KE, Barker DF, Willard HF, Physical mapping of 60 DNA markers in the p21.1----q21.3 region of the human X chromosome. Genomics11:352-63 1991 |
| PubMed ID: 1685139 |
| Passage Frozen |
3 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|
|