Description:
TRANSLOCATED CHROMOSOME
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Chromosome Abnormalities |
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Cell Type
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Fibroblast
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Transformant
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Untransformed
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Race
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White
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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46,XX,inv(5)(pter>p13::q13>p13::q13> qter),t(17;22)(17qter>17p13::22q11> 22qter;22pter>22q11::17p13>17pter)
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
3 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Cytogenetics |
Chromosome 17: TRANSLOCATION Breakpoint 17p13 t(17;22)17p13 |
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Chromosome 22: TRANSLOCATION Breakpoint 22q11 t(17;22)22q11 |
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Chromosome 5: INVERSION Breakpoint 5p13 inv(5)5p13 |
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Chromosome 5: INVERSION Breakpoint 5q13 inv(5)5q13 |
| Remarks |
See GM03197 Lymphoid; no hematological disorder; habitual aborter; normal phenotype |
| ten Hoeve J, Morris C, Poustka A, Groffen J, Heisterkamp N, Isolation of NotI sites from chromosome 22q11. Genomics18:588-97 1993 |
| PubMed ID: 7905853 |
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| McBride OW, Merry D, Givol D, The gene for human p53 cellular tumor antigen is located on chromosome 17 short arm (17p13). Proc Natl Acad Sci U S A83:130-4 1986 |
| PubMed ID: 3001719 |
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| Testa JR, Rowley JD, Hawkins C, Yu RL, Aronson MM, Mulivor RA, Greene AE, Coriell LL, A balanced translocation (17;22) and a pericentric inversion of chromosome 5. Repository identification No. GM3196. Cytogenet Cell Genet27:270 1980 |
| PubMed ID: 7438788 |
| Passage Frozen |
3 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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