Description:
WILMS TUMOR 1; WT1
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases |
| Class |
Heritable Cancer Syndromes and other Cancers |
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Cell Type
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Fibroblast
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Transformant
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Untransformed
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
2 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11 |
Gessler and Bruns (Genomics 5:43-55,1989) employed DNA from this cell culture to construct a long range restriction map of part of the short arm of chromosome 11 including the WAGR region using pulsed-field gel electrophoresis and a number of infrequently cutting restriction enzymes. |
| |
| Remarks |
Aniridia, genitourinary abnormalities, and mental retardation; XX phenotype - 46,XY karyotype (X-linked familial testicular feminization); pedigree contains several females with no menses |
| Gessler M, Bruns GA, A physical map around the WAGR complex on the short arm of chromosome 11. Genomics5:43-55 1989 |
| PubMed ID: 2570029 |
| Passage Frozen |
2 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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