GM06318
Somatic cell hybrid
Description:
NIGMS MAPPING PANEL #2, VERSION 3, HUMAN/RODENT SOMATIC CELL HYBRIDS
NIGMS HUMAN/RODENT SOMATIC CELL HYBRID MAPPING PANEL #2 DNA
NIGMS HUMAN/RODENT SOMATIC CELL HYBRID MINI MAPPING PANEL #2 DNA
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Human/Chinese hamster somatic cell hybrid retaining the human X chromosome
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for Xp22.3p22.2, DXS85; Xp22.32 (also Yp11.3), MIC2; and Xq28, DXS15. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for Xp22.32, STS, Xq13.3, PGK1, and Xq28, G6PD. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for Xp11.3, DXS1003, Xq12, DXS453, Xq13.3, PGK1, Xq21.3, DXS3, Xq26, HPRT, and Xq28, G6PD. |
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| GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for Yp11.3 (also Xp22.32), DXYS20, and Yq12, DYZ1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 1p36, PND; 1p36 (also 3p21), DNF15S1/S2; and 1q32, REN. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 1p13, NGFB, and 1q32, REN. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 2p13, TGFA, 2p23, POMC, and 2q13, IL1A PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 2q37, ALPP. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 3q21, TF. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 3p22-p21.33, GLB1, and 3q26.2-q27, GLUT2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 4p16.2p15.1, D4S20. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 4p15.3, QDPR, and 4q11-q13, AFP. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 5p14-p12, C9, and 5q13.3-q14, HMGCR. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 6 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 6q24q27, ESR. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 6p21.3, TNFA, and 6q24-q27, MAS1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 7 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 7pterp15, ASSP11. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 7p12, EGFR, and 7q31.3, CFTR. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 8 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 8p21-p11.2, LHRH, 8p23-p22, DEF1, and 8q24, TG. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 9 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 9p22, IFNB. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 9p22, IFNA, and 9q22.3-q31, ALDOB. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 9p21, D9S270, 9pter-p22, D9S178, and 9pter-q12, RLN1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 10 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 10p11.2, ITGB1, and 10q24, PLAU. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 11p15.5, D11S12; 11q21q22, CLG; and 11q23.3, ETS1. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 11p13, CAT, and 11q21-q22, CLG. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 12 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 12p13.3-p13.2, F8VWF, and 12q22-q24.2, PAH. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 13 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 13q14.3, RB1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 14 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 14q11.2, NP, and 14q32.1, PI. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 15 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 15q21-q22.2, B2M. PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 15q11.2-q12, GABRB3. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 16q13q22.1, MT2. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 16p13.3, HBA, 16q22.1, HPR, and 16q22.1, LCAT. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 17p13.1, TP53, 17q, GAS, 17q21.3-q23, MPO. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 18p11.31-p11.22, TYMS. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 18p11.1-q11.2, D18S44, and 18q12.1, TTR. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 19 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 19p13.2, LDLR, and 19q13.2, APOC2 |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 20 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 20pter-p12, PRNP, and 20q12-q13.11, ADA. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 21q22.1q22.2, D21S17. Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 21q22.3, ITGB2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
Dubois & Naylor (Genomics 16:315-319,1993) reported that PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 22q11.2, IGLC2. |
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| TESTIS DETERMINING LOCUS |
Lau and Chan (Am J Hum Genet 45:942-952,1989) performed PCR amplification of the zinc-finger-Y (ZFY) and zinc-finger-X (ZFX) specific sequences present in the genomic DNA of this somatic cell hybrid. The results of their Southern blot hybridizations showed the presence of only a ZFX specific DNA fragment. |
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| Remarks |
Human fetal fibroblast fused with HPRT-deficient Chinese hamster, CH65691, using Sendai virus; selected in HAT medium; retains only the human X chromosome in 100% of cells and no other human isozyme markers; culture grows as a monolayer |
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| PubMed ID: 9466997 |
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| Hansen RS, Canfield TK, Stanek AM, Keitges EA, Gartler SM, Reactivation of XIST in normal fibroblasts and a somatic cell hybrid: abnormal localization of XIST RNA in hybrid cells. Proc Natl Acad Sci U S A95:5133-8 1998 |
| PubMed ID: 9560241 |
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| Sprinkle TJ, Stone AA, Venema RC, Denslow ND, Caldwell C, Ryan JW, Assignment of the membrane-bound human aminopeptidase P gene (XPNPEP2) to
chromosome Xq25. Genomics50(1):114-6 1998 |
| PubMed ID: 9628831 |
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| Esposito T, Gianfrancesco F, Ciccodicola A, D'Esposito M, Nagaraja R, Mazzarella R, D'Urso M, Forabosco A, Escape from X inactivation of two new genes associated with DXS6974E and DXS7020E. Genomics43:183-90 1997 |
| PubMed ID: 9244435 |
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| Mills FC, Harindranath N, Mitchell M, Max EE, Enhancer complexes located downstream of both human immunoglobulin Calpha genes. J Exp Med186:845-58 1997 |
| PubMed ID: 9294139 |
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| Schwarz H, Arden K, Lotz M, CD137, a member of the tumor necrosis factor receptor family, is located on chromosome 1p36, in a cluster of related genes, and colocalizes with several malignancies Biochemical and biophysical research communications235:699-703 1997 |
| PubMed ID: 9207223 |
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| Young AC, Chavez M, Giambernardi TA, Mattern V, McGill JR, Harris JM, Sarosdy MF, Patel P, Sakaguchi AY, Organization and expression of human telomere repeat binding factor genes. Somat Cell Mol Genet23:275-86 1997 |
| PubMed ID: 9542529 |
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| Boycott KM, Halley GR, Schlessinger D, Bech-Hansen NT, A 2-megabase physical contig incorporating 43 DNA markers on the human X chromosome at p11.23-p11.22 from ZNF21 to DXS255. Genomics33:488-97 1996 |
| PubMed ID: 8661008 |
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| D'Esposito M, Ciccodicola A, Gianfrancesco F, Esposito T, Flagiello L, Mazzarella R, Schlessinger D, D'Urso M, A synaptobrevin-like gene in the Xq28 pseudoautosomal region undergoes X inactivation. Nat Genet13:227-9 1996 |
| PubMed ID: 8640232 |
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| Hansen RS, Canfield TK, Fjeld AD, Gartler SM, Role of late replication timing in the silencing of X-linked genes. Hum Mol Genet5:1345-53 1996 |
| PubMed ID: 8872476 |
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| Hardas BD, Zhao X, Zhang J, Longqing X, Stoll S, Elder JT, Assignment of psoriasin to human chromosomal band 1q21: coordinate overexpression of clustered genes in psoriasis. J Invest Dermatol106:753-8 1996 |
| PubMed ID: 8618016 |
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| Hirai M, Kusuda J, Hashimoto K, Assignment of human ADP ribosylation factor (ARF) genes ARF1 and ARF3 to chromosomes 1q42 and 12q13, respectively. Genomics34(2):263-5 1996 |
| PubMed ID: 8661066 |
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| Srivastava AK, Montonen O, Saarialho-Kere U, Chen E, Baybayan P, Pispa J, Limon J, Schlessinger D, Kere J, Fine mapping of the EDA gene: a translocation breakpoint is associated with a CpG island that is transcribed. Am J Hum Genet58:126-32 1996 |
| PubMed ID: 8554048 |
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| Thorner PS, Zheng K, Kalluri R, Jacobs R, Hudson BG, Coordinate gene expression of the alpha3, alpha4, and alpha5 chains of collagen type IV. Evidence from a canine model of X-linked nephritis with a COL4A5 gene mutation. J Biol Chem271(23):13821-8 1996 |
| PubMed ID: 8662866 |
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| Arden KC, Viars CS, Fu K, Rozen R, Localization of short/branched chain acyl-CoA dehydrogenase (ACADSB) to human chromosome 10 Genomics25:743-5 1995 |
| PubMed ID: 7759115 |
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| Arden KC, Viars CS, Weiss S, Argentin S, Nemer M, Localization of the human B-type natriuretic peptide precursor (NPPB) gene to chromosome 1p36. Genomics26:385-9 1995 |
| PubMed ID: 7601467 |
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| Hansen RS, Canfield TK, Gartler SM, Reverse replication timing for the XIST gene in human fibroblasts. Hum Mol Genet4:813-20 1995 |
| PubMed ID: 7633440 |
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| Philippe C, Arnould C, Sloan F, van Bokhoven H, van der Velde-Visser SD, Chery M, Ropers HH, Gilgenkrantz S, Monaco AP, Cremers FP, A high-resolution interval map of the q21 region of the human X chromosome. Genomics27:539-43 1995 |
| PubMed ID: 7558039 |
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| Das S, Gitschier J, Identification of a TXREB pseudogene (TXREBP) located between the genes for p55 (MPP1) and G6PD on Xq28. Genomics21:275-278 1994 |
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| De Plaen E, Arden K, Traversari C, Gaforio JJ, Szikora JP, De Smet C, Brasseur F, van der Bruggen P, Lethé B, Lurquin C, Structure, chromosomal localization, and expression of 12 genes of the MAGE family Immunogenetics40:360-9 1994 |
| PubMed ID: 7927540 |
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| Kass S, MacRae C, Graber HL, Sparks EA, McNamara D, Boudoulas H, Basson CT, Baker PB 3rd, Cody RJ, Fishman MC, et al, A gene defect that causes conduction system disease and dilated cardiomyopathy maps to chromosome 1p1-1q1. Nat Genet7:546-51 1994 |
| PubMed ID: 7951328 |
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| Seltmann M, Bohm M, Walter T, Schmitz G, Patterson D, Wieland I, Isolation of region specific single-copy probes from human chromosome 1q23-->1q25. Cytogenet Cell Genet67:46-51 1994 |
| PubMed ID: 8187551 |
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| Weiss J, Rubinfeld B, Polakis PG, McCormick F, Cavenee WK, Arden KC, The RAP1GA1 locus for human Rap1-GTPase activating protein 1 maps to chromosome 1p36.1-->p35. Cytogenet Cell Genet66:18-21 1994 |
| PubMed ID: 8275700 |
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| Drwinga HL, Toji LH, Kim CH, Greene AE, Mulivor RA, NIGMS human/rodent somatic cell hybrid mapping panels 1 and 2. Genomics16:311-4 1993 |
| PubMed ID: 8314568 |
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| Figlewicz DA, Delattre O, Guellaen G, Krizus A, Thomas G, Zucman J, Rouleau GA, Mapping of human gamma-glutamyl transpeptidase genes on chromosome 22 and other human autosomes. Genomics17:299-305 1993 |
| PubMed ID: 8104871 |
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| Hansen RS, Canfield TK, Lamb MM, Gartler SM, Laird CD, Association of fragile X syndrome with delayed replication of the FMR1 gene. Cell73:1403-9 1993 |
| PubMed ID: 8324827 |
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| Kaneko K, Warren ST, Miyatake T, Tsuji S, Isolation of 353 NotI-linking clones and 62 DNA markers (DXS607-DXS668) from human chromosome Xq24-->qter. Cytogenet Cell Genet64:5-8 1993 |
| PubMed ID: 8508680 |
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| Philippe C, Nguyen VC, Slim R, Holvoet-Vermaut L, Hors-Cayla MC, Bernheim A, Rearrangements between irradiated chromosomes in three-species radiation hybrid cell lines revealed by two-color in situ hybridization. Hum Genet92:11-7 1993 |
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| Aplan PD, Raimondi SC, Kirsch IR, Disruption of the SCL gene by a t(1;3) translocation in a patient with T cell acute lymphoblastic leukemia. J Exp Med176:1303-10 1992 |
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| Gorski JL, Boehnke M, Reyner EL, Burright EN, A radiation hybrid map of the proximal short arm of the human X chromosome spanning incontinentia pigmenti 1 (IP1) translocation breakpoints. Genomics14:657-65 1992 |
| PubMed ID: 1427892 |
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| Gorski JL, Burright EN, Reyner EL, Goodfellow PN, Burgess DL, Isolation of DNA markers from a region between incontinentia pigmenti 1 (IP1) X-chromosomal translocation breakpoints by a comparative PCR analysis of a radiation hybrid subclone mapping panel. Genomics14:649-56 1992 |
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| Kaneko K, Kobayashi H, Onodera O, Miyatake T, Tsuji S, Genomic organization of a cDNA (QM) demonstrating an altered mRNA level in nontumorigenic Wilms' microcell hybrid cells and its localization to Xq28. Hum Mol Genet1:529-33 1992 |
| PubMed ID: 1339145 |
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| Arenstorf HP, Kandpal RP, Baskaran N, Parimoo S, Tanaka Y, Kitajima S, Yasukochi Y, Weissman SM, Construction and characterization of a NotI-BsuE linking library from the human X chromosome. Genomics11:115-23 1991 |
| PubMed ID: 1765370 |
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| Green ED, Mohr RM, Idol JR, Jones M, Buckingham JM, Deaven LL, Moyzis RK, Olson MV, Systematic generation of sequence-tagged sites for physical mapping of human chromosomes: application to the mapping of human chromosome 7 using yeast artificial chromosomes. Genomics11:548-64 1991 |
| PubMed ID: 1837788 |
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| Nakamura N, Sugino H, Takahara K, Jin C, Fukushige S, Matsubara K, Endogenous retroviral LTR DNA sequences as markers for individual human chromosomes. Cytogenet Cell Genet57:18-22 1991 |
| PubMed ID: 1713140 |
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| Phaneuf D, Labelle Y, Bérubé D, Arden K, Cavenee W, Gagné R, Tanguay RM, Cloning and expression of the cDNA encoding human fumarylacetoacetate hydrolase, the enzyme deficient in hereditary tyrosinemia: assignment of the gene to chromosome 15 American journal of human genetics48:525-35 1991 |
| PubMed ID: 1998338 |
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| Arden KC, Boutin JM, Djiane J, Kelly PA, Cavenee WK, The receptors for prolactin and growth hormone are localized in the same region of human chromosome 5 Cytogenetics and cell genetics53:161-5 1990 |
| PubMed ID: 2369845 |
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| Testa JR, Park M, Blair DG, Kalbakji A, Arden K, Vande Woude GF, Analysis by pulsed field gel electrophoresis reveals complex rearrangements in two MET alleles in a chemically-treated human cell line, MNNG-HOS Oncogene5:1565-71 1990 |
| PubMed ID: 2250912 |
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| Zacksenhaus E, Sheinin R, Wang HS, Localization of the human A1S9 gene complementing the ts A1S9 mouse L- cell defect in DNA replication and cell cycle progression to Xp11.2---- p11.4. Cytogenet Cell Genet53:20-2 1990 |
| PubMed ID: 2323223 |
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| Lau YF, Chan KM, The putative testis-determining factor and related genes are expressed as discrete-sized transcripts in adult gonadal and somatic tissues. Am J Hum Genet45:942-52 1989 |
| PubMed ID: 2511751 |
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| Zacksenhaus E, Sheinin R, Molecular cloning of human A1S9 locus: an X-linked gene essential for progression through S phase of the cell cycle. Somat Cell Mol Genet15:545-53 1989 |
| PubMed ID: 2595454 |
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| Bonifas JM, Morley BJ, Oakey RE, Kan YW, Epstein EH Jr, Cloning of a cDNA for steroid sulfatase: frequent occurrence of gene deletions in patients with recessive X chromosome-linked ichthyosis. Proc Natl Acad Sci U S A84:9248-51 1987 |
| PubMed ID: 3480541 |
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| Griffin CA, Emanuel BS, Hansen JR, Cavenee WK, Myers JC, Human collagen genes encoding basement membrane alpha 1 (IV) and alpha 2 (IV) chains map to the distal long arm of chromosome 13. Proc Natl Acad Sci U S A84:512-6 1987 |
| PubMed ID: 3025878 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Sodium Pyruvate 1ml/100ml |
| Supplement |
Thymidine 1.6 x 10-5M |
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