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GM08854 Somatic cell hybrid

Description:

ADDITIONAL HUMAN/MOUSE SOMATIC CELL HYBRIDS

Affected:

No Data

Sex:

No Data

Age:

No Data

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • External Links
  • Culture Protocols

Overview

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Repository NIGMS Human Genetic Cell Repository
Subcollection Hybrids
Cell Type Somatic cell hybrid
Transformant Untransformed
Family Member 21
Confirmation Clinical summary/Case history
ISCN Mouse/human somatic cell hybrid retaining human chromosome #21 (1-5 copies/cell) & unidentifiable fragments
Remarks Line WAV 17; normal diploid human fibroblast, WI-38, fused with HPRT and APRT deficient mouse cell line A9 using Sendai virus; selected in alanosine-adenine medium; retains an intact human chromosome #21 in 100% of cells

Characterizations

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IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis
 
GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for Xq28, DXS15.
 
GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for Yp11.3 (also Xp22.32), DXYS20, and Yq12, DYZ1.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1 DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 1p36 (also 3p21), DNF15S1/S2, and 1q32, REN.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 3p25, RAF1, and 3q21, TF.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 21q11, D21S26, and 21q22.3, BCEI.
 

Phenotypic Data

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Remarks Line WAV 17; normal diploid human fibroblast, WI-38, fused with HPRT and APRT deficient mouse cell line A9 using Sendai virus; selected in alanosine-adenine medium; retains an intact human chromosome #21 in 100% of cells

Publications

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Lopes M, Louzada S, Ferreira D, Veríssimo G, Eleutério D, Gama-Carvalho M, Chaves R, Human Satellite 1A analysis provides evidence of pericentromeric transcription BMC biology21:28 2022
PubMed ID: 36755311
 
van Sluis M, Gailín MÓ, McCarter JGW, Mangan H, Grob A, McStay B, Human NORs, comprising rDNA arrays and functionally conserved distal elements, are located within dynamic chromosomal regions Genes & development21:28 2019
PubMed ID: 31727772
 
Zeng W, Chen YY, Newkirk DA, Wu B, Balog J, Kong X, Ball AR, Zanotti S, Tawil R, Hashimoto N, Mortazavi A, van der Maarel SM, Yokomori K, Genetic and epigenetic characteristics of FSHD-associated 4q and 10q D4Z4 that are distinct from non-4q/10q D4Z4 homologs Human mutation35:998-1010 2013
PubMed ID: 24838473
 
Coskun AK, van Maanen M, Nguyen V, Sutton RE, Human chromosome 2 carries a gene required for production of infectious human immunodeficiency virus type 1 Journal of virology80:3406-15 2006
PubMed ID: 16537608
 
Roizès G, Human centromeric alphoid domains are periodically homogenized so that they vary substantially between homologues Mechanism and implications for centromere functioning Nucleic acids research34:1912-24 2006
PubMed ID: 16598075
 
Small KM, Brown KM, Seman CA, Theiss CT, Liggett SB, Complex haplotypes derived from noncoding polymorphisms of the intronless alpha2A-adrenergic gene diversify receptor expression Proceedings of the National Academy of Sciences of the United States of America103:5472-7 2006
PubMed ID: 16567612
 
Chastain-Moore AM, Roberts T, Trott DA, Newbold RF, Ornelles DA, An activity associated with human chromosome 21 permits nuclear colocalization of the adenovirus E1B-55K and E4orf6 proteins and promotes viral late gene expression. J Virol77(14):8087-98 2003
PubMed ID: 12829847
 
Anderson MJ, Viars CS, Czekay S, Cavenee WK, Arden KC, Cloning and characterization of three human forkhead genes that comprise an FKHR-like gene subfamily. Genomics47:187-99 1998
PubMed ID: 9479491
 
Davies JP, Cotter PD, Ioannou YA, Cloning and mapping of human Rab7 and Rab9 cDNA sequences and identification of a Rab9 pseudogene. Genomics41:131-4 1997
PubMed ID: 9126495
 
Gonzalez IL, Sylvester JE, Beyond ribosomal DNA: on towards the telomere. Chromosoma105:431-7 1997
PubMed ID: 9211970
 
Jones MH, Zhang Y, Tirosvoutis KN, Davey PM, Webster AR, Walsh D, Spurr NK, Affara NA, Chromosomal assignment of 311 sequences transcribed in human adult testis. Genomics40:155-67 1997
PubMed ID: 9070934
 
Schwarz H, Arden K, Lotz M, CD137, a member of the tumor necrosis factor receptor family, is located on chromosome 1p36, in a cluster of related genes, and colocalizes with several malignancies Biochemical and biophysical research communications235:699-703 1997
PubMed ID: 9207223
 
Arden KC, Viars CS, Weiss S, Argentin S, Nemer M, Localization of the human B-type natriuretic peptide precursor (NPPB) gene to chromosome 1p36. Genomics26:385-9 1995
PubMed ID: 7601467
 
Bermingham JR Jr, Arden KC, Naumova AK, Sapienza C, Viars CS, Fu XD, Khotz J, Manley JL, Rosenfeld MG, Chromosomal localization of mouse and human genes encoding the splicing factors ASF/SF2 (SFRS1) and SC-35 (SFRS2). Genomics29:70-9 1995
PubMed ID: 8530103
 
Kermouni A, Van Roost E, Arden KC, Vermeesch JR, Weiss S, Godelaine D, Flint J, Lurquin C, Szikora JP, Higgs DR, The IL-9 receptor gene (IL9R): genomic structure, chromosomal localization in the pseudoautosomal region of the long arm of the sex chromosomes, and identification of IL9R pseudogenes at 9qter, 10pter, 16pter, and 18pter Genomics29:371-82 1995
PubMed ID: 8666384
 
Reston JT, Hu XL, Macina RA, Spais C, Riethman HC, Structure of the terminal 300 kb of DNA from human chromosome 21q. Genomics26:31-8 1995
PubMed ID: 7782083
 
Saito M, Helin K, Valentine MB, Griffith BB, Willman CL, Harlow E, Look AT, Amplification of the E2F1 transcription factor gene in the HEL erythroleukemia cell line. Genomics25:130-8 1995
PubMed ID: 7774910
 
De Plaen E, Arden K, Traversari C, Gaforio JJ, Szikora JP, De Smet C, Brasseur F, van der Bruggen P, Lethé B, Lurquin C, Structure, chromosomal localization, and expression of 12 genes of the MAGE family Immunogenetics40:360-9 1994
PubMed ID: 7927540
 
Fukushima A, Okubo K, Sugino H, Hori N, Matoba R, Niiyama T, Murakawa K, Yoshii J, Yokoyama M, Matsubara K, Chromosomal assignment of HepG2 3'-directed partial cDNA sequences by Southern blot hybridization using monochromosomal hybrid cell panels. Genomics22:127-36 1994
PubMed ID: 7959758
 
Hendershot LM, Valentine VA, Lee AS, Morris SW, Shapiro DN, Localization of the gene encoding human BiP/GRP78, the endoplasmic reticulum cognate of the HSP70 family, to chromosome 9q34. Genomics20:281-4 1994
PubMed ID: 8020977
 
Huebner K, Kastury K, Druck T, Salcini AE, Lanfrancone L, Pelicci G, Lowenstein E, Li W, Park SH, Cannizzaro L, et al, Chromosome locations of genes encoding human signal transduction adapter proteins, Nck (NCK), Shc (SHC1), and Grb2 (GRB2). Genomics22:281-7 1994
PubMed ID: 7806213
 
Weiss J, Rubinfeld B, Polakis PG, McCormick F, Cavenee WK, Arden KC, The RAP1GA1 locus for human Rap1-GTPase activating protein 1 maps to chromosome 1p36.1-->p35. Cytogenet Cell Genet66:18-21 1994
PubMed ID: 8275700
 
Chong SS, Kristjansson K, Zoghbi HY, Hughes MR, Molecular cloning of the cDNA encoding a human renal sodium phosphate transport protein and its assignment to chromosome 6p21.3-p23. Genomics18:355-359 1993
PubMed ID: 8288239
 
Figlewicz DA, Delattre O, Guellaen G, Krizus A, Thomas G, Zucman J, Rouleau GA, Mapping of human gamma-glutamyl transpeptidase genes on chromosome 22 and other human autosomes. Genomics17:299-305 1993
PubMed ID: 8104871
 
Hendricks-Taylor LR, Bachinski LL, Siciliano MJ, Fertitta A, Trask B, de Jong PJ, Ledbetter DH, Darlington GJ, The CCAAT/enhancer binding protein (C/EBP alpha) gene (CEBPA) maps to human chromosome 19q13.1 and the related nuclear factor NF-IL6 (C/EBP beta) gene (CEBPB) maps to human chromosome 20q13.1. Genomics14:12-7 1992
PubMed ID: 1427819
 
Morris SW, Nelson N, Valentine MB, Shapiro DN, Look AT, Kozlosky CJ, Beckmann MP, Cerretti DP, Assignment of the genes encoding human interleukin-8 receptor types 1 and 2 and an interleukin-8 receptor pseudogene to chromosome 2q35. Genomics14:685-91 1992
PubMed ID: 1427896
 
Ledbetter SA, Garcia-Heras J, Ledbetter DH, "PCR-karyotype" of human chromosomes in somatic cell hybrids. Genomics8:614-22 1990
PubMed ID: 2276735
 
Tan YH, Tischfield J, Ruddle FH, The linkage of genes for the human interferon-induced antiviral protein and indophenol oxidase-B traits to chromosome G-21. J Exp Med137:317-30 1973
PubMed ID: 4346649

External Links

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dbSNP dbSNP ID: 11147

Culture Protocols

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Split Ratio 1:5
Temperature 37 C
Percent CO2 8%
Medium Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent
Serum 10% fetal bovine serum Not inactivated
Substrate None specified
Subcultivation Method trypsin-EDTA
Supplement -
Pricing
International/Commercial/For-profit:
$373.00USD
U.S. Academic/Non-profit/Government:
$216.00USD
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