GM10613
Fibroblast from Fetal, Unspecified
Description:
TRANSLOCATED CHROMOSOME
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Chromosome Abnormalities |
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Biopsy Source
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Unspecified
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Cell Type
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Fibroblast
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Tissue Type
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Fetal
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Fetal, Unspecified
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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46,XX,t(20;21)(20pter>20q11.2:: 21p11.2>21pter;21qter>21p11.2:: 20q11.2>20qter)
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
4 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Cytogenetics |
Chromosome 20: TRANSLOCATION Breakpoint 20q11 t(20;21)20q11 |
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Chromosome 21: TRANSLOCATION Breakpoint 21p11 t(20;21)21p11 |
| Remarks |
First trimester spontaneous abortion; in situ hybridization with probe D2021 for chromosome #20 centromere utilized to confirm breakpoints; slow growing culture |
| Passage Frozen |
4 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's MEM with Hank's BSS, with 26mM Hepes; ambient CO2 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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