GM11689
Somatic cell hybrid
Description:
ADDITIONAL HUMAN/MOUSE SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Family Member
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13
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Confirmation
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Karyotypic analysis and Southern blot hybridization
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ISCN
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Human/mouse hybrid retaining an isodicentric human chromosome #13 in 88% of cells
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for Xp22.32 and Yp11.3, MIC2, and Xq28, DXS15. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 1p36, PND. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 2p25, D2S1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 10 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 10q24qter, PLAU. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 13 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 13q34, D13S3. |
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| Remarks |
Line HA(13)A; produced by the transfer of neo-marked human chromosome into mouse A9 cells by microcell-mediated chromosome transfer: grown in medium containing G418; retains an isodicentric human chromosome #13 in 88% of cells |
| Wangsa D, Braun R, Stuelten CH, Brown M, Bauer KM, Emons G, Weston LA, Hu Y, Yang HH, Vila-Casadesús M, Lee MP, Brauer P, Warner L, Upender M, Hummon AB, Camps J, Ried T, Induced Chromosomal Aneuploidy Results in Global and Consistent Deregulation of the Transcriptome of Cancer Cells Neoplasia (New York, NY)21:721-729 2019 |
| PubMed ID: 31174021 |
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| Zeng W, Chen YY, Newkirk DA, Wu B, Balog J, Kong X, Ball AR, Zanotti S, Tawil R, Hashimoto N, Mortazavi A, van der Maarel SM, Yokomori K, Genetic and epigenetic characteristics of FSHD-associated 4q and 10q D4Z4 that are distinct from non-4q/10q D4Z4 homologs Human mutation35:998-1010 2013 |
| PubMed ID: 24838473 |
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| Coskun AK, van Maanen M, Nguyen V, Sutton RE, Human chromosome 2 carries a gene required for production of infectious human immunodeficiency virus type 1 Journal of virology80:3406-15 2006 |
| PubMed ID: 16537608 |
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| Roizès G, Human centromeric alphoid domains are periodically homogenized so that they vary substantially between homologues Mechanism and implications for centromere functioning Nucleic acids research34:1912-24 2006 |
| PubMed ID: 16598075 |
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| Small KM, Brown KM, Seman CA, Theiss CT, Liggett SB, Complex haplotypes derived from noncoding polymorphisms of the intronless alpha2A-adrenergic gene diversify receptor expression Proceedings of the National Academy of Sciences of the United States of America103:5472-7 2006 |
| PubMed ID: 16567612 |
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| Tiranti V, Hoertnagel K, Carrozzo R, Galimberti C, Munaro M, Granatiero M, Zelante L, Gasparini P, Marzella R, Rocchi M, Bayona-Bafaluy MP, Enriquez JA, Uziel G, Bertini E, Dionisi-Vici C, Franco B, Meitinger T, Zeviani M, Mutations of SURF-1 in leigh disease associated with cytochrome c oxidase deficiency [In Process Citation] Am J Hum Genet63:1609-21 1998 |
| PubMed ID: 9837813 |
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| Davies JP, Cotter PD, Ioannou YA, Cloning and mapping of human Rab7 and Rab9 cDNA sequences and identification of a Rab9 pseudogene. Genomics41:131-4 1997 |
| PubMed ID: 9126495 |
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| Gonzalez IL, Sylvester JE, Beyond ribosomal DNA: on towards the telomere. Chromosoma105:431-7 1997 |
| PubMed ID: 9211970 |
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| Jones MH, Zhang Y, Tirosvoutis KN, Davey PM, Webster AR, Walsh D, Spurr NK, Affara NA, Chromosomal assignment of 311 sequences transcribed in human adult testis. Genomics40:155-67 1997 |
| PubMed ID: 9070934 |
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| Young AC, Chavez M, Giambernardi TA, Mattern V, McGill JR, Harris JM, Sarosdy MF, Patel P, Sakaguchi AY, Organization and expression of human telomere repeat binding factor genes. Somat Cell Mol Genet23:275-86 1997 |
| PubMed ID: 9542529 |
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| Couch FJ, Rommens JM, Neuhausen SL, Belanger C, Dumont M, Abel K, Bell R, Berry S, Bogden R, Cannon-Albright L, Farid L, Frye C, Hattier T, Janecki T, Jiang P, Kehrer R, Leblanc JF, McArthur-Morrison J, Meney D, Miki Y, Peng Y, Samson C, Schroeder M, Snyder SC, Simard J, et al, Generation of an integrated transcription map of the BRCA2 region on chromosome 13q12-q13. Genomics36:86-99 1996 |
| PubMed ID: 8812419 |
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| Whitney M, Thayer M, Reifsteck C, Olson S, Smith L, Jakobs PM, Leach R, Naylor S, Joenje H, Grompe M, Microcell mediated chromosome transfer maps the Fanconi anaemia group D gene to chromosome 3p. Nat Genet11:341-3 1995 |
| PubMed ID: 7581463 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Geneticin 500ug/ml |
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