GM11713
Somatic cell hybrid
Description:
ADDITIONAL HUMAN/MOUSE SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Family Member
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3
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Mouse/human hybrid retaining human chromosome #3
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 2p25, D2S1, and 2q37, D2S3. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 3p25, RAF1, and 3q26.2qter, TFRC. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 3p14.1-p12, D3S1566, 3p22-p21.33, GLB1, 3p25p24.2, D3S1304, 3q13.1-q13.2, D3S1310, and 3q26.2-q27, GLUT2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 21q11, D21S26, and 21q22.1q22.2, D21S17. |
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| Remarks |
Line A9+3; produced by the transfer of neo-marked human, fetal, female, lung fibroblast chromosomes into mouse A9 cells by microcell-mediated chromosome transfer; grown in medium containing G418; retains human chromosome #3 in 100% of cells |
| Park SH, Bennett-Baker P, Ahmed S, Arlt MF, Ljungman M, Glover TW, Wilson TE, Locus-specific transcription silencing at the FHIT gene suppresses replication stress-induced copy number variant formation and associated replication delay Nucleic acids research49:7507-7524 2021 |
| PubMed ID: 34181717 |
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| Durkin SG, Ragland RL, Arlt MF, Mulle JG, Warren ST, Glover TW, Replication stress induces tumor-like microdeletions in FHIT/FRA3B Proceedings of the National Academy of Sciences of the United States of America105:246-51 2007 |
| PubMed ID: 18162546 |
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| Coskun AK, van Maanen M, Nguyen V, Sutton RE, Human chromosome 2 carries a gene required for production of infectious human immunodeficiency virus type 1 Journal of virology80:3406-15 2006 |
| PubMed ID: 16537608 |
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| Hejna JA,Timmers CD,Reifsteck C,Bruun DA,Lucas LW,Jakobs PM,Toth-Fejel S,Unsworth N,Clemens SL,Garcia DK,Naylor SL,Thayer MJ,Olson SB,Grompe M,Moses R, Localization of the Fanconi anemia complementation group D gene to a 200-kb region on chromosome 3p25.3. Am J Hum Genet66:1540-51 2000 |
| PubMed ID: 10762542 |
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| Cuthbert AP, Bond J, Trott DA, Gill S, Broni J, Marriott A, Khoudoli G, Parkinson EK, Cooper CS, Newbold RF, Telomerase repressor sequences on chromosome 3 and induction of permanent growth arrest in human breast cancer cells [see comments] J Natl Cancer Inst91:37-45 1999 |
| PubMed ID: 9890168 |
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| Julicher K, Marquitan G, Werner N, Bardenheuer W, Vieten L, Brocker F, Topal H, Seeber S, Opalka B, Schutte J, Novel tumor suppressor locus in human chromosome region 3p14.2 [see comments] J Natl Cancer Inst91:1563-8 1999 |
| PubMed ID: 10491433 |
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| Smith L, Liu SJ, Goodrich L, Jacobson D, Degnin C, Bentley N, Carr A, Flaggs G, Keegan K, Hoekstra M, Thayer MJ, Duplication of ATR inhibits MyoD, induces aneuploidy and eliminates radiation-induced G1 arrest [see comments] Nat Genet19:39-46 1998 |
| PubMed ID: 9590286 |
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| Tiranti V, Hoertnagel K, Carrozzo R, Galimberti C, Munaro M, Granatiero M, Zelante L, Gasparini P, Marzella R, Rocchi M, Bayona-Bafaluy MP, Enriquez JA, Uziel G, Bertini E, Dionisi-Vici C, Franco B, Meitinger T, Zeviani M, Mutations of SURF-1 in leigh disease associated with cytochrome c oxidase deficiency [In Process Citation] Am J Hum Genet63:1609-21 1998 |
| PubMed ID: 9837813 |
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| Davies JP, Cotter PD, Ioannou YA, Cloning and mapping of human Rab7 and Rab9 cDNA sequences and identification of a Rab9 pseudogene. Genomics41:131-4 1997 |
| PubMed ID: 9126495 |
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| Rodriguez AM, Rodin D, Nomura H, Morton CC, Weremowicz S, Schneider MC, Identification, localization, and expression of two novel human genes similar to deoxyribonuclease I. Genomics42:507-13 1997 |
| PubMed ID: 9205125 |
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| Whitney M, Thayer M, Reifsteck C, Olson S, Smith L, Jakobs PM, Leach R, Naylor S, Joenje H, Grompe M, Microcell mediated chromosome transfer maps the Fanconi anaemia group D gene to chromosome 3p. Nat Genet11:341-3 1995 |
| PubMed ID: 7581463 |
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| Ning Y, Lovell M, Taylor L, Pereira-Smith OM, Isolation of monochromosomal hybrids following fusion of human diploid fibroblast-derived microcells with mouse A9 cells. Cytogenet Cell Genet60:79-80 1992 |
| PubMed ID: 1582264 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Geneticin 250ug/ml |
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