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GM11715 Somatic cell hybrid

Description:

ADDITIONAL HUMAN/MOUSE SOMATIC CELL HYBRIDS

Affected:

No Data

Sex:

No Data

Age:

No Data

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • External Links
  • Culture Protocols

Overview

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Repository NIGMS Human Genetic Cell Repository
Subcollection Hybrids
Cell Type Somatic cell hybrid
Transformant Untransformed
Family Member 15
Confirmation Karyotypic analysis and In situ hybridization
ISCN Mouse/human hybrid retaining a human chromosome #15 (1-3 copies) in 100% of cells
Remarks Line A9+15; produced by the transfer of neo-marked human, fetal, female, lung fibroblast chromosomes into mouse A9 cells by microcell-mediated chromosome transfer; grown in medium with G418; retains human chromosome #15 (1-3 copies) in 100% of cells

Characterizations

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IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 2p25, D2S1.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 13 DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 13q34, D13S3.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 15 DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 15q25qter, IGF1R. PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 15q11-q12, D15S11.
 

Phenotypic Data

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Remarks Line A9+15; produced by the transfer of neo-marked human, fetal, female, lung fibroblast chromosomes into mouse A9 cells by microcell-mediated chromosome transfer; grown in medium with G418; retains human chromosome #15 (1-3 copies) in 100% of cells

Publications

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Zeng W, Chen YY, Newkirk DA, Wu B, Balog J, Kong X, Ball AR, Zanotti S, Tawil R, Hashimoto N, Mortazavi A, van der Maarel SM, Yokomori K, Genetic and epigenetic characteristics of FSHD-associated 4q and 10q D4Z4 that are distinct from non-4q/10q D4Z4 homologs Human mutation35:998-1010 2013
PubMed ID: 24838473
 
Coskun AK, van Maanen M, Nguyen V, Sutton RE, Human chromosome 2 carries a gene required for production of infectious human immunodeficiency virus type 1 Journal of virology80:3406-15 2006
PubMed ID: 16537608
 
Matsuura S, Matsumoto Y, Morishima K, Izumi H, Matsumoto H, Ito E, Tsutsui K, Kobayashi J, Tauchi H, Kajiwara Y, Hama S, Kurisu K, Tahara H, Oshimura M, Komatsu K, Ikeuchi T, Kajii T, Monoallelic BUB1B mutations and defective mitotic-spindle checkpoint in seven families with premature chromatid separation (PCS) syndrome. Am J Med Genet A140(4):358-67 2006
PubMed ID: 16411201
 
Gabriel JM, Higgins MJ, Gebuhr TC, Shows TB, Saitoh S, Nicholls RD, A model system to study genomic imprinting of human genes. Proc Natl Acad Sci U S A95:14857-62 1998
PubMed ID: 9843980
 
Trask BJ, Friedman C, Martin-Gallardo A, Rowen L, Akinbami C, Blankenship J, Collins C, Giorgi D, Iadonato S, Johnson F, Kuo WL, Massa H, Morrish T, Naylor S, Nguyen OT, Rouquier S, Smith T, Wong DJ, Youngblom J, van den Engh G, Members of the olfactory receptor gene family are contained in large blocks of DNA duplicated polymorphically near the ends of human chromosomes. Hum Mol Genet7:13-26 1998
PubMed ID: 9384599
 
Davies JP, Cotter PD, Ioannou YA, Cloning and mapping of human Rab7 and Rab9 cDNA sequences and identification of a Rab9 pseudogene. Genomics41:131-4 1997
PubMed ID: 9126495
 
Dyomin VG, Rao PH, Dalla-Favera R, Chaganti RS, BCL8, a novel gene involved in translocations affecting band 15q11-13 in diffuse large-cell lymphoma. Proc Natl Acad Sci U S A94(11):5728-32 1997
PubMed ID: 9159141
 
Gonzalez IL, Sylvester JE, Beyond ribosomal DNA: on towards the telomere. Chromosoma105:431-7 1997
PubMed ID: 9211970
 
Jones MH, Zhang Y, Tirosvoutis KN, Davey PM, Webster AR, Walsh D, Spurr NK, Affara NA, Chromosomal assignment of 311 sequences transcribed in human adult testis. Genomics40:155-67 1997
PubMed ID: 9070934
 
Whitney M, Thayer M, Reifsteck C, Olson S, Smith L, Jakobs PM, Leach R, Naylor S, Joenje H, Grompe M, Microcell mediated chromosome transfer maps the Fanconi anaemia group D gene to chromosome 3p. Nat Genet11:341-3 1995
PubMed ID: 7581463
 
Tomlinson IM, Cook GP, Carter NP, Elaswarapu R, Smith S, Walter G, Buluwela L, Rabbitts TH, Winter G, Human immunoglobulin VH and D segments on chromosomes 15q11.2 and 16p11.2. Hum Mol Genet3:853-60 1994
PubMed ID: 7951227
 
Ning Y, Lovell M, Taylor L, Pereira-Smith OM, Isolation of monochromosomal hybrids following fusion of human diploid fibroblast-derived microcells with mouse A9 cells. Cytogenet Cell Genet60:79-80 1992
PubMed ID: 1582264

External Links

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dbSNP dbSNP ID: 11629

Culture Protocols

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Split Ratio 1:5
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent
Serum 10% fetal bovine serum Not inactivated
Substrate None specified
Subcultivation Method trypsin-EDTA
Supplement Geneticin 250ug/ml
Pricing
International/Commercial/For-profit:
$373.00USD
U.S. Academic/Non-profit/Government:
$216.00USD
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